- [fatty-acyl-CoA synthase(Enzyme) wildtype]
Acetyl_CoA + H+ + 2n_2n_2n_2n_2n_2n_2n_NADPH + n_n_n_n_n_n_n_Malonyl_CoA -> 2n_2n_2n_2n_2n_2n_2n_NADP+ + n_n_n_n_n_n_n_CO2 + n+1_n+1_n+1_n+1_n+1_n+1_n+1_Coenzyme_A + Long_chain_fatty_acid |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 100 mM Potassium phosphate, 4 mM Dithiothreitol, 2.5 mM EDTA, 0.3 mg/ml Bovine serum albumin
PH: 6.5
Temperature: 25 |
- [fatty-acyl-CoA synthase(Enzyme) wildtype]
Acetyl_CoA + n_n_n_n_n_n_n_Malonyl_CoA + 2n_2n_2n_2n_2n_2n_2n_NADPH + H+ -> 2n_2n_2n_2n_2n_2n_2n_NADP+ + n_n_n_n_n_n_n_CO2 + n+1_n+1_n+1_n+1_n+1_n+1_n+1_Coenzyme_A + Long_chain_fatty_acid |
(( (Vmax * S3) ) / (Km + S3)) |
|
buffer: 100 mM Potassium phosphate, 4 mM Dithiothreitol, 2.5 mM EDTA, 0.3 mg/ml Bovine serum albumin
PH: 6.5
Temperature: 25 |
- [fatty-acyl-CoA synthase(Enzyme) wildtype]
n_n_n_n_n_n_n_Malonyl_CoA + H+ + 2n_2n_2n_2n_2n_2n_2n_NADPH + Acetyl_CoA -> n_n_n_n_n_n_n_CO2 + Long_chain_fatty_acid + 2n_2n_2n_2n_2n_2n_2n_NADP+ + n+1_n+1_n+1_n+1_n+1_n+1_n+1_Coenzyme_A |
(( (Vmax * S2) ) / (Km + S2)) |
|
buffer: 100 mM Potassium phosphate, 4 mM Dithiothreitol, 2.5 mM EDTA, 0.3 mg/ml Bovine serum albumin
PH: 6.5
Temperature: 25 |
- [fatty-acyl-CoA synthase(Enzyme) wildtype]
n_n_n_n_n_n_n_Malonyl_CoA + Propanoyl_CoA + 2n_2n_2n_2n_2n_2n_2n_NADPH + H+ -> n+1_n+1_n+1_n+1_n+1_n+1_n+1_Coenzyme_A + Long_chain_fatty_acid + n_n_n_n_n_n_n_CO2 + 2n_2n_2n_2n_2n_2n_2n_NADP+ |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 100 mM Potassium phosphate, 4 mM Dithiothreitol, 2.5 mM EDTA, 0.3 mg/ml Bovine serum albumin
PH: 6.5
Temperature: 25 |
- [fatty-acyl-CoA synthase(Enzyme) wildtype]
H+ + Butanoyl_CoA + 2n_2n_2n_2n_2n_2n_2n_NADPH + n_n_n_n_n_n_n_Malonyl_CoA -> Long_chain_fatty_acid + n_n_n_n_n_n_n_CO2 + n+1_n+1_n+1_n+1_n+1_n+1_n+1_Coenzyme_A + 2n_2n_2n_2n_2n_2n_2n_NADP+ |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 100 mM Potassium phosphate, 4 mM Dithiothreitol, 2.5 mM EDTA, 0.3 mg/ml Bovine serum albumin
PH: 6.5
Temperature: 25 |
- [fatty-acyl-CoA synthase(Enzyme) wildtype]
n_n_n_n_n_n_n_Malonyl_CoA + Octanoyl_CoA + H+ + 2n_2n_2n_2n_2n_2n_2n_NADPH -> Long_chain_fatty_acid + n+1_n+1_n+1_n+1_n+1_n+1_n+1_Coenzyme_A + n_n_n_n_n_n_n_CO2 + 2n_2n_2n_2n_2n_2n_2n_NADP+ |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 100 mM Potassium phosphate, 4 mM Dithiothreitol, 2.5 mM EDTA, 0.3 mg/ml Bovine serum albumin
PH: 6.5
Temperature: 25 |
- [fatty-acyl-CoA synthase(Enzyme) wildtype]
Hexanoyl_CoA + n_n_n_n_n_n_n_Malonyl_CoA + 2n_2n_2n_2n_2n_2n_2n_NADPH + H+ -> n+1_n+1_n+1_n+1_n+1_n+1_n+1_Coenzyme_A + Long_chain_fatty_acid + n_n_n_n_n_n_n_CO2 + 2n_2n_2n_2n_2n_2n_2n_NADP+ |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 100 mM Potassium phosphate, 4 mM Dithiothreitol, 2.5 mM EDTA, 0.3 mg/ml Bovine serum albumin
PH: 6.5
Temperature: 25 |