EC: 1.14.11.1

gamma-butyrobetaine dioxygenase (4-trimethylammoniobutanoate,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating))

enzyme lambda metabolic reaction experiment
uniprot:O75936 [Fe2+]

2_Oxoglutarate + 4_Trimethylammoniobutanoate + O2 -> Succinate + CO2 + Carnitine		 (( (Vmax * (Fe ^ n)) ) / (( (Fe ^ n) ) + (S_half ^ n))) buffer: 14 mM Potassium phosphate, 14 mM sodium ascorbate, 1.4 mg/ml Catalase
PH: 6.5
Temperature: 37
uniprot:O75936 [Fe2+]

O2 + 4_Trimethylammoniobutanoate + 2_Oxoglutarate -> Succinate + Carnitine + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: 14 mM Potassium phosphate, 14 mM sodium ascorbate, 1.4 mg/ml Catalase
PH: 6.5
Temperature: 37
uniprot:O75936 [Fe2+]

2_Oxoglutarate + O2 + 4_Trimethylammoniobutanoate -> CO2 + Succinate + Carnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 14 mM Potassium phosphate, 14 mM sodium ascorbate, 1.4 mg/ml Catalase
PH: 6.5
Temperature: 37
uniprot:O75936 [Fe2+]

O2 + 4_Trimethylammoniobutanoate + 2_Oxoglutarate -> Succinate + CO2 + Carnitine		 (( (Vmax * B) ) / (Km + B)) buffer: 14 mM Potassium phosphate, 14 mM sodium ascorbate, 1.4 mg/ml Catalase
PH: 6.5
Temperature: 37
uniprot:O75936 [Fe2+]

O2 + 2_Oxoglutarate + 4_Trimethylammoniobutanoate -> Carnitine + CO2 + Succinate		 (( (Vmax * B) ) / (Km + B)) buffer: 14 mM Potassium phosphate, 14 mM sodium ascorbate, 1.4 mg/ml Catalase
PH: 6.5
Temperature: 37
uniprot:O75936 [Fe2+]

2_Oxoglutarate + O2 + 4_Trimethylammoniobutanoate -> CO2 + Carnitine + Succinate		 (( (Vmax * B) ) / (Km + B)) buffer: 14 mM Potassium phosphate, 14 mM sodium ascorbate, 1.4 mg/ml Catalase
PH: 6.5
Temperature: 37
uniprot:O75936 [Fe2+]

O2 + 2_Oxoglutarate + 4_Trimethylammoniobutanoate -> Carnitine + Succinate + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: 14 mM Potassium phosphate, 14 mM sodium ascorbate, 1.4 mg/ml Catalase
PH: 6.5
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-3621 γ-butyrobetaine degradation
BioCyc:META_PWY-6100 L-carnitine biosynthesis
BioCyc:HUMAN_PWY-6100 L-carnitine biosynthesis
BioCyc:MOUSE_PWY-6100 L-carnitine biosynthesis
Reactome:R-MMU-1430728 Metabolism
Reactome:R-CEL-71291 Amino acid and derivative metabolism
Reactome:R-CEL-71262 Carnitine synthesis
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-71262 Carnitine synthesis
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71262 Carnitine synthesis
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-RNO-71262 Carnitine synthesis
Reactome:R-CEL-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
PathBank:SMP0087289 Carnitine Synthesis
PathBank:SMP0000465 Carnitine Synthesis