EC: 1.13.11.16

3-carboxyethylcatechol 2,3-dioxygenase (3-(2,3-dihydroxyphenyl)propanoate:oxygen 1,2-oxidoreductase (decyclizing))

enzyme lambda metabolic reaction experiment
uniprot:P17295 [Fe2+]

2,3_Dihydroxytoluene + O2 -> 2_Hydroxy_6_keto_2,4_heptadienoate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 8
Temperature: 20
uniprot:P17295 [Fe2+]

O2 + 3_Ethylcatechol -> 2_Hydroxy_6_oxo_octa_2,4_dienoate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 8
Temperature: 20
uniprot:P17295 [Fe2+]

3_(2,3_Dihydroxyphenyl)propanoate + O2 -> 2_Hydroxy_6_oxonona_2,4_diene_1,9_dioate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 8
Temperature: 20
uniprot:P0ABR9 [Fe2+]

O2 + 3_Ethylcatechol -> 2_Hydroxy_6_oxo_octa_2,4_dienoate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 8
Temperature: 20
uniprot:P0ABR9 [Fe2+]

O2 + trans_2,3_Dihydroxycinnamate -> 2_Hydroxy_6_ketononatrienedioate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 8
Temperature: 20
uniprot:P0ABR9 [Fe2+]

O2 + Methyl_2,3_dihydroxyphenylpropionate -> 2_Hydroxy_6_oxonona_2,4_dienoic_acid_9_methylester		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 8
Temperature: 20
uniprot:P0ABR9 [Fe2+]

O2 + 2,3_Dihydroxyphenoxyacetic_acid -> 2_Hydroxyhexa_2,4_dienoic_acid_6_carboxymethylester		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 8
Temperature: 20
uniprot:P0ABR9 [Fe2+]

O2 + 3_Propylcatechol -> 2_Hydroxy_6_oxo_nona_2,4_dienoate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 8
Temperature: 20
uniprot:P0ABR9 [Fe2+]

O2 + 3_Phenethylcatechol -> 2_Hydroxy_6_oxo_8_phenylocta_2,4_dienoate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 8
Temperature: 20
uniprot:P0ABR9 [3-carboxyethylcatechol 2,3-dioxygenase(Enzyme) wildtype]

3_(2,3_Dihydroxyphenyl)propanoate + O2 -> 2_Hydroxy_6_oxonona_2,4_diene_1,9_dioate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 36
uniprot:P0ABR9 [3-carboxyethylcatechol 2,3-dioxygenase(Enzyme) mutant fusion P181A]

3_(2,3_Dihydroxyphenyl)propanoate + O2 -> 2_Hydroxy_6_oxonona_2,4_diene_1,9_dioate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 36
uniprot:P0ABR9 [3-carboxyethylcatechol 2,3-dioxygenase(Enzyme) wildtype fusion MBP-MhpB]

3_(2,3_Dihydroxyphenyl)propanoate + O2 -> 2_Hydroxy_6_oxonona_2,4_diene_1,9_dioate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 36
uniprot:P0ABR9 [3-carboxyethylcatechol 2,3-dioxygenase(Enzyme) mutant fusion P181H]

3_(2,3_Dihydroxyphenyl)propanoate + O2 -> 2_Hydroxy_6_oxonona_2,4_diene_1,9_dioate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 36
uniprot:P0ABR9 [3-carboxyethylcatechol 2,3-dioxygenase(Enzyme) mutant fusion D114N]

3_(2,3_Dihydroxyphenyl)propanoate + O2 -> 2_Hydroxy_6_oxonona_2,4_diene_1,9_dioate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 36

Pathways

pathway id name
BioCyc:META_PWY-6690 cinnamate and 3-hydroxycinnamate degradation to 2-hydroxypentadienoate
BioCyc:META_HCAMHPDEG-PWY 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation to 2-hydroxypentadienoate
BioCyc:ECOL316407_PWY0-1277 3-phenylpropionate and 3-(3-hydroxyphenyl)propionate degradation
BioCyc:META_PWY0-1277 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation
BioCyc:ECO_HCAMHPDEG-PWY 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation to 2-hydroxypentadienoate
BioCyc:ECOO157_HCAMHPDEG-PWY 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation to 2-oxopent-4-enoate
BioCyc:ECOL316407_PWY-6690 cinnamate and 3-hydroxycinnamate degradation to 2-oxopent-4-enoate
BioCyc:ECO_PWY0-1277 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation
BioCyc:ECO_PWY-6690 cinnamate and 3-hydroxycinnamate degradation to 2-hydroxypentadienoate
BioCyc:ECOO157_PWY0-1277 3-phenylpropionate and 3-(3-hydroxyphenyl)propionate degradation
BioCyc:ECOL316407_HCAMHPDEG-PWY 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation to 2-oxopent-4-enoate
PathBank:SMP0001904 2-Oxopent-4-enoate Metabolism
PathBank:SMP0002049 2-Oxopent-4-enoate Metabolism 2