EC: 1.1.1.133

dTDP-4-dehydrorhamnose reductase (dTDP-6-deoxy-beta-L-mannose:NADP+ 4-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P26392 [Mg2+]

NADPH + H+ + dTDP_4_dehydro_beta_L_rhamnose -> NADP+ + dTDP_L_rhamnose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris-HCl, RmIB and RmIC in 24fold molar excess relative to RmID
PH: 6.5
Temperature: 36
uniprot:P26392 [Mg2+]

dTDP_4_dehydro_beta_L_rhamnose + NADPH + H+ -> dTDP_L_rhamnose + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris-HCl, RmIB and RmIC in 24fold molar excess relative to RmID
PH: 6.5
Temperature: 36
uniprot:P26392 [Mg2+]

H+ + NADPH + dTDP_4_dehydro_beta_L_rhamnose -> NADP+ + dTDP_L_rhamnose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris-HCl, RmIB and RmIC in 24fold molar excess relative to RmID
PH: 6.5
Temperature: 36
uniprot:P26392 [dTDP-4-dehydrorhamnose reductase(Enzyme) wildtype]

H+ + dTDP_4_dehydro_beta_L_rhamnose + NADPH -> dTDP_L_rhamnose + NADP+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 45 mM potassium phosphate, 9 mM MgCl2, 100-fold molar excess of RmlC
PH: 7
Temperature: 25
uniprot:P26392 [dTDP-4-dehydrorhamnose reductase(Enzyme) wildtype]

H+ + dTDP_4_dehydro_beta_L_rhamnose + NADH -> NAD+ + dTDP_L_rhamnose		 (( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (( (Kia * KmB) ) + (KmA * B)) ) + (KmB * A)) ) + (A * B))) buffer: 45 mM potassium phosphate, 9 mM MgCl2, 100-fold molar excess of RmlC
PH: 7
Temperature: 25
uniprot:P26392 [dTDP-4-dehydrorhamnose reductase(Enzyme) wildtype]

H+ + dTDP_4_dehydro_beta_L_rhamnose + NADH -> dTDP_L_rhamnose + NAD+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 45 mM potassium phosphate, 9 mM MgCl2, 100-fold molar excess of RmlC
PH: 7
Temperature: 25
uniprot:Q93F84 [dTDP-4-dehydrorhamnose reductase(Enzyme) wildtype RmlD His-tagged]

NADPH + H+ + dTDP_4_dehydro_beta_L_rhamnose -> NADP+ + dTDP_L_rhamnose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 45 mM potassium phosphate, 9 mM MgCl2, 100-fold molar excess of RmlC from Salmonella enterica LT2
PH: 7
Temperature: 25
uniprot:Q93F84 [dTDP-4-dehydrorhamnose reductase(Enzyme) wildtype RmlD His-tagged]

dTDP_4_dehydro_beta_L_rhamnose + H+ + NADH -> dTDP_L_rhamnose + NAD+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 45 mM potassium phosphate, 9 mM MgCl2, 100-fold molar excess of RmlC from Salmonella enterica LT2
PH: 7
Temperature: 25

Pathways

pathway id name
BioCyc:MTBCDC1551_DTDPRHAMSYN-PWY dTDP-L-rhamnose biosynthesis I
BioCyc:MTBH37RV_PWY-6404 superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis
BioCyc:MTBCDC1551_OANTIGEN-PWY O-antigen building blocks biosynthesis (E. coli)
BioCyc:ECOL316407_DTDPRHAMSYN-PWY dTDP-L-rhamnose biosynthesis I
BioCyc:ECOL316407_OANTIGEN-PWY O-antigen building blocks biosynthesis (E. coli)
BioCyc:META_PWY-7317 superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis
BioCyc:MTBH37RV_DTDPRHAMSYN-PWY dTDP-L-rhamnose biosynthesis
BioCyc:MTBCDC1551_PWY-6404 superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis
BioCyc:META_DTDPRHAMSYN-PWY dTDP-L-rhamnose biosynthesis
BioCyc:ECO_OANTIGEN-PWY O-antigen building blocks biosynthesis (E. coli)
BioCyc:ECO_DTDPRHAMSYN-PWY dTDP-L-rhamnose biosynthesis
BioCyc:ARA_PWY-3221 dTDP-L-rhamnose biosynthesis II
PathBank:SMP0000958 Starch and Sucrose Metabolism
PathBank:SMP0002101 O-Antigen Building Blocks Biosynthesis
PlantCyc:ARA_PWY-3221 dTDP-L-rhamnose biosynthesis II
PlantCyc:PLANT_PWY-3221 dTDP-L-rhamnose biosynthesis II
PlantCyc:PLANT_DTDPRHAMSYN-PWY dTDP-L-rhamnose biosynthesis
PlantCyc:ARA_DTDPRHAMSYN-PWY dTDP-L-rhamnose biosynthesis