EC: 2.4.1.21

starch synthase (glycosyl-transferring) (ADP-glucose:(1->4)-alpha-D-glucan 4-alpha-D-glucosyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q00775 [starch synthase(Enzyme) wildtype S-tagged GBSSI]

Amylopectin + ADP_alpha_D_glucoside -> ADP + Amylopectin		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM bicine, 25 mm potassium acetate, 10 mm dithiothreitol, 5 mm EDTA
PH: 8.5
Temperature: 25
uniprot:O49064 [Citrate]

ADP_alpha_D_glucoside + Amylose -> Amylose + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Bicine-NaOH, 5 mM EDTA, 0.5 mg/ml BSA
PH: 8
Temperature: 37
uniprot:O49064 [Citrate]

ADP_alpha_D_glucoside + Amylopectin -> Amylopectin + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Bicine-NaOH, 5 mM EDTA, 0.5 mg/ml BSA
PH: 8
Temperature: 37
uniprot:O64923 [Citrate]

ADP_alpha_D_glucoside + Glycogen -> ADP + Glycogen		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Bicine-NaOH, 5 mM EDTA, 0.5 mg/ml BSA
PH: 8
Temperature: 37
uniprot:O49064 [Citrate]

Glycogen + ADP_alpha_D_glucoside -> ADP + Glycogen		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Bicine-NaOH, 5 mM EDTA, 0.5 mg/ml BSA
PH: 8
Temperature: 37
uniprot:O49064 [starch synthase(Enzyme) wildtype SSI]

ADP_alpha_D_glucoside + Amylose -> Amylose + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Bicine-NaOH, 5 mM EDTA, 0.5 mg/ml BSA
PH: 8
Temperature: 37
uniprot:O49064 [starch synthase(Enzyme) wildtype SSI]

ADP_alpha_D_glucoside + Amylopectin -> ADP + Amylopectin		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Bicine-NaOH, 5 mM EDTA, 0.5 mg/ml BSA
PH: 8
Temperature: 37
uniprot:O49064 [starch synthase(Enzyme) wildtype SSI]

Glycogen + ADP_alpha_D_glucoside -> Glycogen + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Bicine-NaOH, 5 mM EDTA, 0.5 mg/ml BSA
PH: 8
Temperature: 37
uniprot:Q43847 [starch synthase(Enzyme) wildtype S-tagged SSII]

Amylopectin + ADP_alpha_D_glucoside -> Amylopectin + ADP		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM bicine, 25 mm potassium acetate, 10 mm dithiothreitol, 5 mm EDTA
PH: 8.5
Temperature: 25
uniprot:Q43847 [starch synthase(Enzyme) wildtype S-tagged SSII]

ADP_alpha_D_glucoside + Amylopectin -> Amylopectin + ADP		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM bicine, 25 mm potassium acetate, 10 mm dithiothreitol, 5 mm EDTA
PH: 8.5
Temperature: 25
uniprot:Q43847 [starch synthase(Enzyme) wildtype S-tagged SSII]

ADP_alpha_D_glucoside + Amylopectin -> ADP + Amylopectin		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM bicine, 25 mm potassium acetate, 10 mm dithiothreitol, 5 mm EDTA
PH: 8.5
Temperature: 25
uniprot:O64923 [Citrate]

Amylose + ADP_alpha_D_glucoside -> ADP + Amylose		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Bicine-NaOH, 5 mM EDTA, 0.5 mg/ml BSA
PH: 8
Temperature: 37
uniprot:O64923 [Citrate]

ADP_alpha_D_glucoside + Amylopectin -> Amylopectin + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Bicine-NaOH, 5 mM EDTA, 0.5 mg/ml BSA
PH: 8
Temperature: 37
uniprot:O64923 [starch synthase(Enzyme) wildtype SSII]

Amylose + ADP_alpha_D_glucoside -> ADP + Amylose		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Bicine-NaOH, 5 mM EDTA, 0.5 mg/ml BSA
PH: 8
Temperature: 37
uniprot:O64923 [starch synthase(Enzyme) wildtype SSII]

ADP_alpha_D_glucoside + Amylopectin -> Amylopectin + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Bicine-NaOH, 5 mM EDTA, 0.5 mg/ml BSA
PH: 8
Temperature: 37
uniprot:O64923 [starch synthase(Enzyme) wildtype SSII]

Glycogen + ADP_alpha_D_glucoside -> ADP + Glycogen		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Bicine-NaOH, 5 mM EDTA, 0.5 mg/ml BSA
PH: 8
Temperature: 37
- [starch synthase(Enzyme) wildtype]

Amylopectin + ADP_alpha_D_glucoside -> Amylopectin + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Bicine (N,N'-bis(2-hydroxyethyl)glycine),10 mM potassium acetate, 10 mM GSH, 5 mM EDTA
PH: 8.5
Temperature: 37
- [starch synthase(Enzyme) wildtype]

Glycogen + ADP_alpha_D_glucoside -> Glycogen + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Bicine (N,N'-bis(2-hydroxyethyl)glycine), 10 mM potassium acetate, 10 mM GSH, 5 mM EDTA
PH: 8.5
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-622 starch biosynthesis
BioCyc:VCHO_GLYCOGENSYNTH-PWY glycogen biosynthesis I (from ADP-D-Glucose)
BioCyc:SYNEL_GLYCOGENSYNTH-PWY glycogen biosynthesis I (from ADP-D-Glucose)
BioCyc:PDIF272563_GLYCOGENSYNTH-PWY glycogen biosynthesis I (from ADP-D-Glucose)
BioCyc:ECO_GLYCOGENSYNTH-PWY glycogen biosynthesis I (from ADP-D-Glucose)
BioCyc:ARA_PWY-622 starch biosynthesis
BioCyc:ECOO157_GLYCOGENSYNTH-PWY glycogen biosynthesis I (from ADP-D-Glucose)
BioCyc:AGRO_GLYCOGENSYNTH-PWY glycogen biosynthesis I (from ADP-D-Glucose)
BioCyc:BSUB_GLYCOGENSYNTH-PWY glycogen biosynthesis I (from ADP-D-Glucose)
BioCyc:META_GLYCOGENSYNTH-PWY glycogen biosynthesis I (from ADP-D-Glucose)
BioCyc:ECOL199310_GLYCOGENSYNTH-PWY glycogen biosynthesis I (from ADP-D-Glucose)
BioCyc:ANTHRA_GLYCOGENSYNTH-PWY glycogen biosynthesis I (from ADP-D-Glucose)
BioCyc:ECOL316407_GLYCOGENSYNTH-PWY glycogen biosynthesis I (from ADP-D-Glucose)
WikiPathways:WP2472 Escherichia coli K-12 peripherome
PathBank:SMP0000958 Starch and Sucrose Metabolism
PathBank:SMP0000984 Secondary Metabolites: Trehalose Biosynthesis and Metabolism
Plant Reactome:R-OSA-9030769 Growth and developmental processes
Plant Reactome:R-OSA-9623902 Seed development
Plant Reactome:R-OSA-9031669 Reproductive structure development
Plant Reactome:R-OSA-9626305 Regulatory network of nutrient accumulation
PlantCyc:ARA_PWY-622 starch biosynthesis
PlantCyc:ARA_PWYQT-4466 superpathway of sucrose and starch metabolism I (non-photosynthetic tissue)
PlantCyc:PLANT_PWY-622 starch biosynthesis
PlantCyc:POTATO_PWY-622 starch biosynthesis
PlantCyc:PLANT_PWYQT-4466 superpathway of sucrose and starch metabolism I (non-photosynthetic tissue)
PathBank:SMP0121323 Starch and Sucrose Metabolism
PathBank:SMP0121327 Secondary Metabolites: Trehalose Biosynthesis and Metabolism