EC: 1.1.1.27

L-lactate dehydrogenase ((S)-lactate:NAD+ oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P26283 [Fructose 1,6-bisphosphate]

H+ + Pyruvate + NADH -> NAD+ + D_Lactate		 (( (Vmax * (S ^ n)) ) / (( (S05 ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Maleate
PH: 6.2
Temperature: 32
uniprot:P26283 [Fructose 1,6-bisphosphate]

Pyruvate + NADH + H+ -> D_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (( (S05 ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Maleate
PH: 6.2
Temperature: 32
uniprot:P26283 [Fructose 1,6-bisphosphate]

H+ + NADH + Pyruvate -> D_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (( (S05 ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Maleate
PH: 6.2
Temperature: 32
uniprot:P26283 [ATP]

Pyruvate + NADH + H+ -> NAD+ + D_Lactate		 (( (Vmax * (S ^ n)) ) / (( (S05 ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Maleate
PH: 6.2
Temperature: 32
uniprot:P26283 [ATP]

H+ + Pyruvate + NADH -> NAD+ + D_Lactate		 (( (Vmax * (S ^ n)) ) / (( (S05 ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Maleate
PH: 6.2
Temperature: 32
uniprot:P26283 [ATP]

Pyruvate + H+ + NADH -> NAD+ + D_Lactate		 (( (Vmax * (S ^ n)) ) / (( (S05 ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Maleate
PH: 6.2
Temperature: 32
uniprot:P26283 [ATP]

H+ + Pyruvate + NADH -> D_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (( (S05 ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Maleate
PH: 6.2
Temperature: 32
uniprot:P26283 [Fructose 1,6-bisphosphate]

NADH + H+ + Pyruvate -> D_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (( (S05 ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Maleate
PH: 6.2
Temperature: 32
uniprot:P26283 [Fructose 1,6-bisphosphate]

NADH + Pyruvate + H+ -> NAD+ + D_Lactate		 (( (Vmax * (S ^ n)) ) / (( (S05 ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Maleate
PH: 6.2
Temperature: 32
uniprot:P26283 [Fructose 1,6-bisphosphate]

H+ + Pyruvate + NADH -> D_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (( (S05 ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Maleate
PH: 6.2
Temperature: 32
uniprot:P26283 [Fructose 1,6-bisphosphate]

H+ + NADH + Pyruvate -> D_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (( (S05 ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Maleate
PH: 6.2
Temperature: 32
uniprot:P26283 [Fructose 1,6-bisphosphate]

H+ + NADH + Pyruvate -> NAD+ + D_Lactate		 (( (Vmax * (S ^ n)) ) / (( (S05 ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Maleate
PH: 6.2
Temperature: 32
uniprot:P26283 [ATP]

H+ + Pyruvate + NADH -> D_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (( (S05 ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Maleate
PH: 6.2
Temperature: 32
uniprot:P26283 [ATP]

Pyruvate + NADH + H+ -> NAD+ + D_Lactate		 (( (Vmax * (S ^ n)) ) / (( (S05 ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Maleate
PH: 6.2
Temperature: 32
uniprot:P26283 [Fructose 1,6-bisphosphate]

NADH + Pyruvate + H+ -> NAD+ + D_Lactate		 (( (Vmax * (S ^ n)) ) / (( (S05 ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Maleate
PH: 6.2
Temperature: 32
uniprot:P26283 [Fructose 1,6-bisphosphate]

H+ + NADH + Pyruvate -> D_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (( (S05 ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Maleate
PH: 6.2
Temperature: 32
uniprot:P26283 [Fructose 1,6-bisphosphate]

Pyruvate + NADH + H+ -> D_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (( (S05 ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Maleate
PH: 6.2
Temperature: 32
uniprot:P26283 [Fructose 1,6-bisphosphate]

NADH + Pyruvate + H+ -> D_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (( (S05 ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Maleate
PH: 6.2
Temperature: 32
uniprot:P26283 [Fructose 1,6-bisphosphate]

H+ + NADH + Pyruvate -> D_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (( (S05 ^ n) ) + (S ^ n))) buffer: 20 mM Tris-Maleate
PH: 6.2
Temperature: 32
uniprot:P13774 [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + NADH -> NAD+ + L_Lactate		 (( (( (( (( (( (Vpf * VfPFvLDH) ) * nadhPF) ) * pyrPF) ) / (( (( (KnadhPFvLDH * KpyrPFvLDH) ) * Vpf) ) * Vpf)) ) - (( (( (( (Vpf * VrPFvLDH) ) * lacPF) ) * nadPF) ) / (( (( (KlacPFvLDH * KnadPFvLDH) ) * Vpf) ) * Vpf))) ) / (( (( (1 + (nadPF / (KnadPFvLDH * Vpf))) ) + (nadhPF / (KnadhPFvLDH * Vpf))) ) * (( (1 + (lacPF / (KlacPFvLDH * Vpf))) ) + (pyrPF / (KpyrPFvLDH * Vpf))))) buffer: *
PH: 7
Temperature: 36
uniprot:P13714 [L-lactate dehydrogenase(Enzyme) mutant als::Spt]

Pyruvate + H+ + NADH -> L_Lactate + NAD+		 (( (Vmax * S) ) / (S + Km)) buffer: 0.087 M Tris-maleate
PH: 4.3
Temperature: 30
uniprot:P13714 [L-lactate dehydrogenase(Enzyme) mutant als::Spt]

H+ + NADPH + Pyruvate -> L_Lactate + NADP+		 (( (Vmax * S) ) / (S + Km)) buffer: 0.087 M Tris-maleate
PH: 4.3
Temperature: 30
- [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> Pyruvate + H+ + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 7
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> Pyruvate + H+ + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 7.4
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

NAD+ + L_Lactate -> H+ + NADH + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 8
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> NADH + Pyruvate + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 6.5
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> H+ + Pyruvate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 8
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

NAD+ + L_Lactate -> Pyruvate + NADH + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 6.5
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> NADH + H+ + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 8
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

NAD+ + L_Lactate -> H+ + Pyruvate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 7.4
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

NAD+ + L_Lactate -> Pyruvate + H+ + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 7
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> H+ + NADH + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 7.4
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

NAD+ + L_Lactate -> NADH + H+ + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 8
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> NADH + H+ + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 7
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + NADH + H+ -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 7
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

NADH + H+ + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 6.5
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

H+ + NADH + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 6.5
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

NADH + Pyruvate + H+ -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 7.4
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

H+ + NADH + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 7
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + H+ + NADH -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 8
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

NAD+ + L_Lactate -> NADH + H+ + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 6.5
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + H+ + NADH -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 7
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

H+ + Pyruvate + NADH -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 8
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + H+ + NADH -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 7.4
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

NAD+ + L_Lactate -> H+ + Pyruvate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 7.4
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> Pyruvate + NADH + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 7
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + NADH + H+ -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 7.4
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

NADH + H+ + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 8
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + H+ + NADH -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 7
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + NADH + H+ -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 6.5
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> Pyruvate + H+ + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 6.5
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

H+ + Pyruvate + NADH -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 6.5
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

NADH + H+ + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 7.4
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + NADH + H+ -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES-KOH, 0.25 M sucrose, 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 µg/ml rotenone
PH: 8
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

H+ + NADH + Oxaloacetate -> L_Malate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7
Temperature: 30
- [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> H+ + Pyruvate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 8.8
Temperature: 22
- [L-lactate dehydrogenase(Enzyme) wildtype]

H+ + Pyruvate + NADH -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.8
Temperature: 22
- [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + H+ + NADH -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.8
Temperature: 22
- [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> NADH + Pyruvate + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 8.8
Temperature: 22
uniprot:P26283 [Fructose 1,6-bisphosphate]

NADH + Pyruvate + H+ -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 6.2
Temperature: 22
uniprot:D8KFT1 [Fructose 1,6-bisphosphate]

H+ + NADH + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * S) ) / (Km + S)) buffer: *
PH: 7
Temperature: 36
- [Fructose 1,6-bisphosphate]

H+ + Pyruvate + NADH -> NAD+ + L_Lactate		 (( (Vmax * S) ) / (Km + S)) buffer: *
PH: 7
Temperature: 36
uniprot:D8KFT1 [L-lactate dehydrogenase(Enzyme) wildtype ldhB]

Pyruvate + NADH + H+ -> NAD+ + L_Lactate		 (( (Vmax * S) ) / (Km + S)) buffer: *
PH: 7
Temperature: 36
- [L-lactate dehydrogenase(Enzyme) wildtype ldhB]

H+ + Pyruvate + NADH -> L_Lactate + NAD+		 (( (Vmax * S) ) / (Km + S)) buffer: *
PH: 7
Temperature: 36
uniprot:D8KFT1 [L-lactate dehydrogenase(Enzyme) wildtype ldhB]

NADH + Pyruvate + H+ -> L_Lactate + NAD+		 (( (Vmax * S) ) / (Km + S)) buffer: *
PH: 7
Temperature: 36
- [Fructose 1,6-bisphosphate]

Pyruvate + H+ + NADH -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 90 mM Tris-maleate
PH: 6.9
Temperature: 25
- [Fructose 1,6-bisphosphate]

H+ + Pyruvate + NADH -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 90 mM Tris-maleate
PH: 6.9
Temperature: 25
- [Fructose 1,6-bisphosphate]

Pyruvate + H+ + NADH -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 90 mM phosphate
PH: 6.9
Temperature: 25
- [Fructose 1,6-bisphosphate]

NADH + H+ + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 90 mM Tris-maleate
PH: 6.9
Temperature: 25
- [Fructose 1,6-bisphosphate]

NADH + Pyruvate + H+ -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 90 mM Tris-maleate
PH: 6.9
Temperature: 25
- [Fructose 1,6-bisphosphate]

NAD+ + L_Lactate -> NADH + Pyruvate + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 90 mM Triethanolamine-hydrochloride
PH: 7.9
Temperature: 25
- [Fructose 1,6-bisphosphate]

NAD+ + L_Lactate -> H+ + NADH + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 90 mM Triethanolamine-hydrochloride
PH: 7.9
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

NAD+ + L_Lactate -> NADH + Pyruvate + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 90 mM Triethanolamine-hydrochloride
PH: 7.9
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> H+ + NADH + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 90 mM Triethanolamine-hydrochloride
PH: 7.9
Temperature: 25
- [Fructose 1,6-bisphosphate]

NADH + H+ + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 90 mM Tris-maleate
PH: 6.9
Temperature: 25
- [Fructose 1,6-bisphosphate]

Pyruvate + H+ + NADH -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 90 mM Tris-maleate
PH: 6.9
Temperature: 25
- [Fructose 1,6-bisphosphate]

NADH + Pyruvate + H+ -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 90 mM Tris-maleate
PH: 6.9
Temperature: 25
- [Fructose 1,6-bisphosphate]

NADH + Pyruvate + H+ -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 90 mM Tris-maleate
PH: 6.9
Temperature: 25
- [Fructose 1,6-bisphosphate]

H+ + Pyruvate + NADH -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 90 mM Tris-maleate
PH: 6.9
Temperature: 25
- [Fructose 1,6-bisphosphate]

NADH + H+ + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 90 mM Tris-maleate
PH: 6.9
Temperature: 25
- [Fructose 1,6-bisphosphate]

Pyruvate + H+ + NADH -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 90 mM Tris-maleate
PH: 6.9
Temperature: 25
uniprot:Q27797 [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + NADH + H+ -> NAD+ + L_Lactate		 (( (( (E * kcat) ) * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) buffer: 100 mM Tris
PH: 7.5
Temperature: 25
uniprot:Q27797 [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + Reduced_3_acetylpyridine_adenine_dinucleotide + H+ -> L_Lactate + 3_Acetylpyridine_adenine_dinucleotide		 (( (( (E * kcat) ) * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) buffer: 100 mM Tris
PH: 7.5
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype isoenzyme II]

NADH + H+ + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES-KOH
PH: 7.3
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype isoenzyme II]

NAD+ + L_Lactate -> H+ + Pyruvate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-maleate
PH: 8.2
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype isoenzyme II]

NAD+ + L_Lactate -> NADH + H+ + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-maleate
PH: 8.2
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype isoenzyme II]

L_Lactate + NAD+ -> H+ + NADH + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-maleate
PH: 8.2
Temperature: 25
uniprot:Q27743 [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + NADH + H+ -> L_Lactate + NAD+		 (( (( (kcat * E) ) * B) ) / (( (B + Km) ) + (( (B ^ 2) ) / Ki))) buffer: 50 mM Tris-HCl, 50 mM KCl, 1 mM EDTA, 3% PEG 6000, 0.03% NaN3
PH: 7.5
Temperature: 36
uniprot:Q7SI97 [L-lactate dehydrogenase(Enzyme) wildtype]

H+ + Pyruvate + NADH -> NAD+ + L_Lactate		 (( (( (kcat * E) ) * B) ) / (( (B + Km) ) + (( (B ^ 2) ) / Ki))) buffer: 50 mM Tris-HCl, 50 mM KCl, 1 mM EDTA, 3% PEG 6000, 0.03% NaN3
PH: 7.5
Temperature: 36
uniprot:Q27743 [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + NADH + H+ -> NAD+ + L_Lactate		 (( (( (kcat * E) ) * A) ) / (( (A + Km) ) + (( (A ^ 2) ) / Ki))) buffer: 50 mM Tris-HCl, 50 mM KCl, 1 mM EDTA, 3% PEG 6000, 0.03% NaN3
PH: 7.5
Temperature: 36
uniprot:Q7SI97 [L-lactate dehydrogenase(Enzyme) wildtype]

H+ + Pyruvate + NADH -> NAD+ + L_Lactate		 (( (( (kcat * E) ) * A) ) / (( (A + Km) ) + (( (A ^ 2) ) / Ki))) buffer: 50 mM Tris-HCl, 50 mM KCl, 1 mM EDTA, 3% PEG 6000, 0.03% NaN3
PH: 7.5
Temperature: 36
- [L-lactate dehydrogenase(Enzyme) wildtype isoenzyme I]

NADH + H+ + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES-KOH
PH: 7.3
Temperature: 25
- [L-lactate dehydrogenase(Enzyme) wildtype isoenzyme I]

L_Lactate + NAD+ -> NADH + Pyruvate + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Bicine-KOH
PH: 8.8
Temperature: 25
- [Fructose 1,6-bisphosphate]

Pyruvate + NADH + H+ -> L_Lactate + NAD+		 (( (( (Vmax * (1 - exp((( (0 - kapp) ) * t)))) ) * A) ) / (Ka + A)) buffer: 0.1 M Mes
PH: 6.1
Temperature: 26
- [Fructose 1,6-bisphosphate]

H+ + Pyruvate + NADH -> L_Lactate + NAD+		 (( (( (Vmax * (1 - exp((( (0 - kapp) ) * t)))) ) * A) ) / (Ka + A)) buffer: 0.1 M Mes
PH: 6.1
Temperature: 26
- [Fructose 1,6-bisphosphate]

NADH + Pyruvate + H+ -> NAD+ + L_Lactate		 (( (( (Vmax * (1 - exp((( (0 - kapp) ) * t)))) ) * A) ) / (Ka + A)) buffer: 0.1 M Mes
PH: 6.1
Temperature: 26
- [Fructose 1,6-bisphosphate]

Pyruvate + H+ + NADH -> L_Lactate + NAD+		 (( (( (Vmax * (1 - exp((( (0 - kapp) ) * t)))) ) * A) ) / (Ka + A)) buffer: 0.1 M Mes
PH: 6.1
Temperature: 26
- [Co2+]

H+ + Pyruvate + NADH -> L_Lactate + NAD+		 (( (( (Vmax * (1 - exp((( (0 - kapp) ) * t)))) ) * A) ) / (Ka + A)) buffer: 0.1 M Mes
PH: 5.5
Temperature: 26
- [Co2+]

NADH + H+ + Pyruvate -> L_Lactate + NAD+		 (( (( (Vmax * (1 - exp((( (0 - kapp) ) * t)))) ) * A) ) / (Ka + A)) buffer: 0.1 M Mes
PH: 5.5
Temperature: 26
- [Co2+]

H+ + Pyruvate + NADH -> NAD+ + L_Lactate		 (( (( (Vmax * (1 - exp((( (0 - kapp) ) * t)))) ) * A) ) / (Ka + A)) buffer: 0.1 M Mes
PH: 5.5
Temperature: 26
- [Co2+]

H+ + NADH + Pyruvate -> NAD+ + L_Lactate		 (( (( (Vmax * (1 - exp((( (0 - kapp) ) * t)))) ) * A) ) / (Ka + A)) buffer: 0.1 M Mes
PH: 5.5
Temperature: 26
uniprot:P26283 [Fructose 1,6-bisphosphate]

H+ + Pyruvate + NADH -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris-maleate
PH: 6.2
Temperature: 36
uniprot:P26283 [Fructose 1,6-bisphosphate]

H+ + Pyruvate + NADH -> L_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris-maleate
PH: 6.2
Temperature: 36
uniprot:P26283 [Fructose 1,6-bisphosphate]

Pyruvate + H+ + NADH -> L_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris-maleate
PH: 6.2
Temperature: 36
uniprot:P26283 [Fructose 1,6-bisphosphate]

H+ + Pyruvate + NADH -> L_Lactate + NAD+		 (( (Vmax * (M ^ n)) ) / (( (M_half ^ n) ) + (M ^ n))) buffer: 20 mM Tris-maleate
PH: 6.2
Temperature: 36
uniprot:P26283 [Fructose 1,6-bisphosphate]

Pyruvate + H+ + NADH -> L_Lactate + NAD+		 (( (Vmax * (M ^ n)) ) / (( (M_half ^ n) ) + (M ^ n))) buffer: 20 mM Tris-maleate
PH: 6.2
Temperature: 36
uniprot:P26283 [Fructose 1,6-bisphosphate]

H+ + Pyruvate + NADH -> NAD+ + L_Lactate		 (( (Vmax * (M ^ n)) ) / (( (M_half ^ n) ) + (M ^ n))) buffer: 20 mM Tris-maleate
PH: 6.2
Temperature: 36
uniprot:P26283 [Fructose 1,6-bisphosphate]

NADH + Pyruvate + H+ -> L_Lactate + NAD+		 (( (Vmax * (M ^ n)) ) / (( (M_half ^ n) ) + (M ^ n))) buffer: 20 mM Tris-maleate
PH: 6.2
Temperature: 36
uniprot:P26283 [Fructose 1,6-bisphosphate]

Pyruvate + NADH + H+ -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris-maleate
PH: 6.2
Temperature: 36
uniprot:P26283 [Fructose 1,6-bisphosphate]

H+ + NADH + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris-maleate
PH: 6.2
Temperature: 36
uniprot:P26283 [Fructose 1,6-bisphosphate]

Pyruvate + H+ + NADH -> L_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris-maleate
PH: 6.2
Temperature: 36
uniprot:P26283 [Fructose 1,6-bisphosphate]

NADH + Pyruvate + H+ -> L_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris-maleate
PH: 6.2
Temperature: 36
uniprot:P26283 [Fructose 1,6-bisphosphate]

NADH + H+ + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Tris-maleate
PH: 6.2
Temperature: 36
uniprot:P07195 [L-lactate dehydrogenase(Enzyme) wildtype LDH-H]

NADH + 2_Oxobutyrate + H+ -> NAD+ + 2_Hydroxybutyrate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 7.5
Temperature: 25
uniprot:P56511 [L-lactate dehydrogenase(Enzyme) wildtype]

NAD+ + L_Lactate -> H+ + Pyruvate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Tris-HCl
PH: 8.2
Temperature: 30
uniprot:P56511 [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> H+ + Pyruvate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Tris-HCl
PH: 8.2
Temperature: 30
- [L-lactate dehydrogenase(Enzyme) wildtype]

NAD+ + L_Lactate -> NADH + H+ + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Tris-HCl
PH: 8.2
Temperature: 30
- [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> Pyruvate + NADH + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Glycine-NaOH
PH: 9.8
Temperature: 30
- [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> NADH + Pyruvate + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Tris-HCl
PH: 8.2
Temperature: 30
- [L-lactate dehydrogenase(Enzyme) wildtype]

NAD+ + L_Lactate -> Pyruvate + NADH + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Tris-HCl
PH: 8.2
Temperature: 30
uniprot:Q27743 [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + H+ + NADH -> NAD+ + L_Lactate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 7.5
Temperature: 25
uniprot:Q27743 [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> NADH + H+ + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 9.2
Temperature: 25
uniprot:P13491 [L-lactate dehydrogenase(Enzyme) wildtype]

NAD+ + L_Lactate -> NADH + H+ + Pyruvate		 (Vf / (( (( (1 + (Ka / A)) ) + (Kb / B)) ) + (Kab / (A * B)))) buffer: 0.15 M Tris-Cl
PH: 7.15
Temperature: 28
uniprot:P13491 [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + NADH + H+ -> L_Lactate + NAD+		 (Vr / (( (( (1 + (Kc / C)) ) + (Kd / D)) ) + (Kcd / (C * D)))) buffer: 0.15 M Tris-Cl
PH: 7.15
Temperature: 28
uniprot:P00338 [L-lactate dehydrogenase(Enzyme) wildtype LDH-M]

NAD+ + L_Lactate -> H+ + Pyruvate + NADH		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 9.2
Temperature: 25
uniprot:P00338 [L-lactate dehydrogenase(Enzyme) wildtype LDH-M]

Pyruvate + H+ + NADH -> L_Lactate + NAD+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 7.5
Temperature: 25
uniprot:Q27743 [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> NADH + Pyruvate + H+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 9.2
Temperature: 25
uniprot:Q27743 [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + NADH + H+ -> NAD+ + L_Lactate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 7.5
Temperature: 25
uniprot:Q27743 [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + 3_Acetylpyridine_adenine_dinucleotide -> Pyruvate + Reduced_3_acetylpyridine_adenine_dinucleotide + H+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 9.2
Temperature: 25
uniprot:P13491 [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> H+ + Pyruvate + NADH		 (E * (( (( (( (1 / k5) ) + (1 / (k1 * A))) ) + (( (( (( (1 + (D / Kii)) ) * (1 + (( (k4 * D) ) / k5))) ) / k2) ) * B)) ) + (( (( (( (( (k2 * (1 + (( (k4 * D) ) / k5))) ) / k1) ) * k3) ) * A) ) * B))) buffer: *
PH: 7
Temperature: 36
uniprot:P13491 [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> NADH + H+ + Pyruvate		 (E * (( (( (( (1 / k5) ) + (1 / (k1 * A))) ) + (( (( (( (1 + (D / Kii)) ) * (1 + (( (k4 * D) ) / k5))) ) / k2) ) * B)) ) + (( (( (( (( (k2 * (1 + (( (k4 * D) ) / k5))) ) / k1) ) * k3) ) * A) ) * B))) buffer: *
PH: 7
Temperature: 36
uniprot:P13491 [L-lactate dehydrogenase(Enzyme) wildtype]

H+ + Pyruvate + NADH -> L_Lactate + NAD+		 (E * (( (( (( (1 / k5) ) + (1 / (k1 * A))) ) + (( (( (( (1 + (D / Ki)) ) * (1 + (( (k4 * D) ) / k5))) ) / k2) ) * B)) ) + (( (( (( (( (k2 * (1 + (( (k4 * D) ) / k5))) ) / k1) ) * k3) ) * A) ) * B))) buffer: *
PH: 7
Temperature: 36
uniprot:P13491 [L-lactate dehydrogenase(Enzyme) wildtype]

NADH + Pyruvate + H+ -> L_Lactate + NAD+		 (E * (( (( (( (1 / k5) ) + (1 / (k1 * A))) ) + (( (( (( (1 + (D / Ki)) ) * (1 + (( (k4 * D) ) / k5))) ) / k2) ) * B)) ) + (( (( (( (( (k2 * (1 + (( (k4 * D) ) / k5))) ) / k1) ) * k3) ) * A) ) * B))) buffer: *
PH: 7
Temperature: 36
uniprot:P13491 [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + NADH + H+ -> NAD+ + L_Lactate		 (Vr / (( (( (1 + (Kc / C)) ) + (Kd / D)) ) + (Kcd / (C * D)))) buffer: 0.25 M Phosphate
PH: 6.8
Temperature: 28
uniprot:P13491 [L-lactate dehydrogenase(Enzyme) wildtype]

NAD+ + L_Lactate -> H+ + NADH + Pyruvate		 (Vf / (( (( (1 + (Ka / A)) ) + (Kb / B)) ) + (Kab / (A * B)))) buffer: 0.25 M Phosphate
PH: 6.8
Temperature: 28
uniprot:P00338 [L-lactate dehydrogenase(Enzyme) wildtype LDH-M]

NAD+ + L_Lactate -> H+ + Pyruvate + NADH		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 9.2
Temperature: 25
uniprot:P07195 [L-lactate dehydrogenase(Enzyme) wildtype LDH-H]

L_Lactate + NAD+ -> Pyruvate + H+ + NADH		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 9.2
Temperature: 25
uniprot:P00338 [L-lactate dehydrogenase(Enzyme) wildtype LDH-M]

H+ + NADH + Pyruvate -> L_Lactate + NAD+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 7.5
Temperature: 25
uniprot:P07195 [L-lactate dehydrogenase(Enzyme) wildtype LDH-H]

NADH + H+ + Pyruvate -> L_Lactate + NAD+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 7.5
Temperature: 25
uniprot:P07195 [L-lactate dehydrogenase(Enzyme) wildtype LDH-H]

NADH + H+ + 3_Hydroxypyruvate -> Glycerate + NAD+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 7.5
Temperature: 25
uniprot:P00338 [L-lactate dehydrogenase(Enzyme) wildtype LDH-M]

NADH + H+ + 3_Hydroxypyruvate -> Glycerate + NAD+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 7.5
Temperature: 25
uniprot:Q27743 [L-lactate dehydrogenase(Enzyme) wildtype]

NADH + H+ + 3_Hydroxypyruvate -> NAD+ + Glycerate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 7.5
Temperature: 25
uniprot:P00338 [L-lactate dehydrogenase(Enzyme) wildtype LDH-M]

2_Oxobutyrate + H+ + NADH -> 2_Hydroxybutyrate + NAD+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 7.5
Temperature: 25
uniprot:Q27743 [L-lactate dehydrogenase(Enzyme) wildtype]

H+ + 2_Oxobutyrate + NADH -> NAD+ + 2_Hydroxybutyrate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 7.5
Temperature: 25
uniprot:P07195 [L-lactate dehydrogenase(Enzyme) wildtype LDH-H]

Pyruvate + H+ + NADH -> NAD+ + L_Lactate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 7.5
Temperature: 25
uniprot:P07195 [L-lactate dehydrogenase(Enzyme) wildtype LDH-H]

NAD+ + L_Lactate -> H+ + NADH + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 9.2
Temperature: 25
uniprot:P07195 [L-lactate dehydrogenase(Enzyme) wildtype LDH-H]

3_Acetylpyridine_adenine_dinucleotide + L_Lactate -> H+ + Reduced_3_acetylpyridine_adenine_dinucleotide + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 9.2
Temperature: 25
uniprot:P00338 [L-lactate dehydrogenase(Enzyme) wildtype LDH-M]

3_Acetylpyridine_adenine_dinucleotide + L_Lactate -> H+ + Pyruvate + Reduced_3_acetylpyridine_adenine_dinucleotide		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 9.2
Temperature: 25
uniprot:P13491 [L-lactate dehydrogenase(Enzyme) wildtype]

NADH + H+ + Pyruvate -> NAD+ + L_Lactate		 (E * (( (( (( (1 / k5) ) + (( (( (1 + (( (( (k6 * C) ) / k5) ) * (1 + (B / Kii)))) ) / k1) ) * A)) ) + (1 / (k3 * B))) ) + (k2 * (( (( (( (( (1 + (( (( (k6 * C) ) / k5) ) * (1 + (B / Kii)))) ) / k1) ) * k3) ) * A) ) * B)))) buffer: *
PH: 7
Temperature: 36
uniprot:P13491 [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + H+ + NADH -> L_Lactate + NAD+		 (E * (( (( (( (1 / k5) ) + (( (( (1 + (( (( (k6 * C) ) / k5) ) * (1 + (B / Kii)))) ) / k1) ) * A)) ) + (1 / (k3 * B))) ) + (k2 * (( (( (( (( (1 + (( (( (k6 * C) ) / k5) ) * (1 + (B / Kii)))) ) / k1) ) * k3) ) * A) ) * B)))) buffer: *
PH: 7
Temperature: 36
uniprot:P13491 [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> Pyruvate + NADH + H+		 (E * (( (( (( (1 / k5) ) + (( (( (1 + (( (( (k6 * C) ) / k5) ) * (1 + (B / Ki)))) ) / k1) ) * A)) ) + (1 / (k3 * B))) ) + (k2 * (( (( (( (( (1 + (( (( (k6 * C) ) / k5) ) * (1 + (B / Ki)))) ) / k1) ) * k3) ) * A) ) * B)))) buffer: *
PH: 7
Temperature: 36
uniprot:P13491 [L-lactate dehydrogenase(Enzyme) wildtype]

NAD+ + L_Lactate -> Pyruvate + H+ + NADH		 (E * (( (( (( (1 / k5) ) + (( (( (1 + (( (( (k6 * C) ) / k5) ) * (1 + (B / Ki)))) ) / k1) ) * A)) ) + (1 / (k3 * B))) ) + (k2 * (( (( (( (( (1 + (( (( (k6 * C) ) / k5) ) * (1 + (B / Ki)))) ) / k1) ) * k3) ) * A) ) * B)))) buffer: *
PH: 7
Temperature: 36
uniprot:P00343 [Fructose 1,6-bisphosphate]

H+ + NADH + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (( (A + Km) ) + (( (A ^ 2) ) / Ki))) buffer: 50 mM Sodium acetate
PH: 5
Temperature: 30
uniprot:P00343 [L-lactate dehydrogenase(Enzyme) wildtype]

H+ + NADH + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Sodium acetate
PH: 5
Temperature: 30
uniprot:P00343 [L-lactate dehydrogenase(Enzyme) mutant H188D]

Pyruvate + NADH + H+ -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Sodium acetate
PH: 5
Temperature: 30
uniprot:P00343 [Fructose 1,6-bisphosphate]

H+ + Pyruvate + NADH -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodium morpholinepropanesulfonic acid
PH: 7
Temperature: 30
uniprot:P00343 [Fructose 1,6-bisphosphate]

Pyruvate + H+ + NADH -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodium morpholinepropanesulfonic acid
PH: 7
Temperature: 30
uniprot:P00343 [2-Oxoglutarate]

NADH + H+ + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Sodium acetate
PH: 5
Temperature: 30
uniprot:P00343 [Oxamate]

NADH + H+ + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Sodium acetate
PH: 5
Temperature: 30
uniprot:P00343 [Fructose 1,6-bisphosphate]

NADH + H+ + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Sodium morpholinepropanesulfonic acid
PH: 7
Temperature: 30
uniprot:P00343 [Fructose 1,6-bisphosphate]

H+ + NADH + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Sodium acetate
PH: 5
Temperature: 30
uniprot:P00343 [Fructose 1,6-bisphosphate]

H+ + NADH + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Sodium morpholinepropanesulfonic acid
PH: 7
Temperature: 30
uniprot:P00343 [Fructose 1,6-bisphosphate]

NADH + Pyruvate + H+ -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (( (A + Km) ) + (( (A ^ 2) ) / Ki))) buffer: 50 mM Sodium acetate
PH: 5
Temperature: 30
uniprot:P00343 [Oxamate]

H+ + Pyruvate + NADH -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodium acetate
PH: 5
Temperature: 30
uniprot:P00343 [Fructose 1,6-bisphosphate]

Pyruvate + NADH + H+ -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodium morpholinepropanesulfonic acid
PH: 7
Temperature: 30
uniprot:P00343 [Fructose 1,6-bisphosphate]

Pyruvate + H+ + NADH -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodium morpholinepropanesulfonic acid
PH: 7
Temperature: 30
uniprot:P00343 [Fructose 1,6-bisphosphate]

H+ + NADH + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Sodium acetate
PH: 5
Temperature: 30
uniprot:P00343 [Fructose 1,6-bisphosphate]

NADH + Pyruvate + H+ -> L_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Sodium acetate
PH: 5
Temperature: 30
uniprot:P00343 [Fructose 1,6-bisphosphate]

Pyruvate + H+ + NADH -> L_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Sodium morpholinepropanesulfonic acid
PH: 7
Temperature: 30
uniprot:P00343 [Fructose 1,6-bisphosphate]

H+ + Pyruvate + NADH -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Sodium morpholinepropanesulfonic acid
PH: 7
Temperature: 30
uniprot:P0CI34 [Fructose 1,6-bisphosphate]

Pyruvate + NADH + H+ -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Mes/KOH
PH: 6
Temperature: 30
uniprot:P0CI34 [Fructose 1,6-bisphosphate]

NADH + Pyruvate + H+ -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Mes/KOH
PH: 7
Temperature: 30
uniprot:P0CI34 [Fructose 1,6-bisphosphate]

NADH + H+ + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Mes/KOH
PH: 7
Temperature: 30
uniprot:P0CI34 [Fructose 1,6-bisphosphate]

Pyruvate + NADH + H+ -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Mes/KOH
PH: 6
Temperature: 30
uniprot:P0CI34 [Fructose 1,6-bisphosphate]

NADH + Pyruvate + H+ -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Mes/KOH
PH: 6
Temperature: 30
uniprot:P0CI34 [Fructose 1,6-bisphosphate]

H+ + Pyruvate + NADH -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Mes/KOH
PH: 7
Temperature: 30
uniprot:A2RKA4 [Fructose 1,6-bisphosphate]

H+ + NADH + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Mes/KOH
PH: 7
Temperature: 30
uniprot:A2RKA4 [Fructose 1,6-bisphosphate]

NADH + Pyruvate + H+ -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Mes/KOH
PH: 6
Temperature: 30
uniprot:A2RKA4 [Fructose 1,6-bisphosphate]

NADH + H+ + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Mes/KOH
PH: 7
Temperature: 30
uniprot:A2RKA4 [Fructose 1,6-bisphosphate]

H+ + NADH + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Mes/KOH
PH: 6
Temperature: 30
uniprot:A2RKA4 [Fructose 1,6-bisphosphate]

Pyruvate + NADH + H+ -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Mes/KOH
PH: 6
Temperature: 30
uniprot:A2RKA4 [Fructose 1,6-bisphosphate]

NADH + H+ + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Mes/KOH
PH: 7
Temperature: 30
- [Hycanthone]

NADH + Pyruvate + H+ -> NAD+ + L_Lactate		 (1 / (( (( (Km / (Vmax * S)) ) * (1 + (I / Ki))) ) + (( (1 / Vmax) ) * (1 + (I / Ki))))) buffer: *
PH: 7
Temperature: 36
- [Hycanthone]

NADH + Pyruvate + H+ -> NAD+ + L_Lactate		 (1 / (( (( (Km / (Vmax * S)) ) * (1 + (I / Ki))) ) + (( (1 / Vmax) ) * (1 + (I / Ki))))) buffer: *
PH: 7
Temperature: 36
- [Hycanthone]

NADH + H+ + Pyruvate -> NAD+ + L_Lactate		 (1 / (( (( (Km / (Vmax * S)) ) * (1 + (I / Ki))) ) + (( (1 / Vmax) ) * (1 + (I / Ki))))) buffer: *
PH: 7
Temperature: 36
- [Hycanthone]

NADH + H+ + Pyruvate -> L_Lactate + NAD+		 (1 / (( (( (Km / (Vmax * S)) ) * (1 + (I / Ki))) ) + (( (1 / Vmax) ) * (1 + (I / Ki))))) buffer: *
PH: 7
Temperature: 36
- [L-lactate dehydrogenase(Enzyme) wildtype isoenzyme-5]

NADH + H+ + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: -
PH: 7
Temperature: 37
uniprot:P56511 [L-lactate dehydrogenase(Enzyme) wildtype]

H2O + L_Lactate + 3_Acetylpyridine_adenine_dinucleotide -> H+ + Pyruvate + Reduced_3_acetylpyridine_adenine_dinucleotide		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Tris
PH: 7.5
Temperature: 25
uniprot:P56511 [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + Deamino_NADH + H+ -> Deamino_NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Tris
PH: 7.5
Temperature: 25
uniprot:P00343 [FDP]

H+ + NADH + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (S05 + (S ^ n))) buffer: 40 mM Imidazole
PH: 6.2
Temperature: 36
uniprot:P00343 [FDP]

NADH + Pyruvate + H+ -> L_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (S05 + (S ^ n))) buffer: 80 mM Acetate
PH: 5.5
Temperature: 36
uniprot:P00343 [FDP]

NADH + Pyruvate + H+ -> L_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (S05 + (S ^ n))) buffer: 80 mM Acetate
PH: 4.8
Temperature: 36
uniprot:P00343 [FDP]

Pyruvate + H+ + NADH -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (S05 + (S ^ n))) buffer: 80 mM Acetate
PH: 5.5
Temperature: 36
- [FDP]

H+ + NADH + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM Acetate
PH: 5.5
Temperature: 36
- [FDP]

Pyruvate + H+ + NADH -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Imidazole
PH: 6.2
Temperature: 36
- [FDP]

NADH + Pyruvate + H+ -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Imidazole
PH: 7
Temperature: 36
- [FDP]

H+ + NADH + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM Acetate
PH: 5.5
Temperature: 36
- [FDP]

H+ + NADH + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM Acetate
PH: 5.5
Temperature: 36
uniprot:P00343 [FDP]

H+ + NADH + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (S05 + (S ^ n))) buffer: 80 mM Acetate
PH: 4.8
Temperature: 36
uniprot:P00343 [FDP]

H+ + NADH + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (S05 + (S ^ n))) buffer: 40 mM Imidazole
PH: 7
Temperature: 36
uniprot:P00343 [FDP]

NADH + Pyruvate + H+ -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (S05 + (S ^ n))) buffer: 40 mM Imidazole
PH: 7
Temperature: 36
uniprot:P00343 [FDP]

H+ + NADH + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (S05 + (S ^ n))) buffer: 40 mM Imidazole
PH: 6.2
Temperature: 36
uniprot:P00343 [FDP]

Pyruvate + NADH + H+ -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (S05 + (S ^ n))) buffer: 80 mM Acetate
PH: 5.5
Temperature: 36
uniprot:P00343 [FDP]

NADH + Pyruvate + H+ -> NAD+ + L_Lactate		 (( (Vmax * S) ) / (Km + S)) buffer: 80 mM Acetate
PH: 4.8
Temperature: 36
- [FDP]

Pyruvate + H+ + NADH -> NAD+ + L_Lactate		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Imidazole
PH: 7
Temperature: 36
- [FDP]

H+ + NADH + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Imidazole
PH: 6.2
Temperature: 36
- [FDP]

H+ + Pyruvate + NADH -> L_Lactate + NAD+		 (( (Vmax * S) ) / (Km + S)) buffer: 80 mM Acetate
PH: 4.8
Temperature: 36
- [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> Pyruvate + NADH + H+		 (( (Vmax * S2) ) / (Km + S2)) buffer: 25 mM Tris-maleate
PH: 8.2
Temperature: 30
- [L-lactate dehydrogenase(Enzyme) wildtype]

NADH + Pyruvate + H+ -> L_Lactate + NAD+		 (( (Vmax * S1) ) / (Km + S1)) buffer: 25 mM Tris-maleate
PH: 7.4
Temperature: 30
- [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + H+ + NADH -> L_Lactate + NAD+		 (( (Vmax * S2) ) / (Km + S2)) buffer: 25 mM Tris-maleate
PH: 7.4
Temperature: 30
- [L-lactate dehydrogenase(Enzyme) wildtype]

H+ + NADH + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * S1) ) / (Km + S1)) buffer: 25 mM Tris-maleate
PH: 5.4
Temperature: 30
- [L-lactate dehydrogenase(Enzyme) wildtype]

Pyruvate + H+ + NADH -> NAD+ + L_Lactate		 (( (Vmax * S2) ) / (Km + S2)) buffer: 25 mM Tris-maleate
PH: 5.4
Temperature: 30
uniprot:P00343 [FDP]

Pyruvate + NADH + H+ -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (S05 + (S ^ n))) buffer: 40 mM Imidazole
PH: 6.2
Temperature: 36
uniprot:P00343 [FDP]

H+ + NADH + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (S05 + (S ^ n))) buffer: 80 mM Acetate
PH: 4.8
Temperature: 36
uniprot:P00343 [FDP]

H+ + NADH + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (S05 + (S ^ n))) buffer: 80 mM Acetate
PH: 4.8
Temperature: 36
uniprot:P00343 [FDP]

H+ + NADH + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM Acetate
PH: 4.8
Temperature: 36
uniprot:P00343 [FDP]

NADH + H+ + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM Acetate
PH: 5.5
Temperature: 36
uniprot:P00343 [FDP]

H+ + Pyruvate + NADH -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM Acetate
PH: 5.5
Temperature: 36
uniprot:P00343 [FDP]

H+ + NADH + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM Acetate
PH: 4.8
Temperature: 36
uniprot:P00343 [FDP]

H+ + NADH + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM Acetate
PH: 4.8
Temperature: 36
uniprot:P00343 [FDP]

Pyruvate + H+ + NADH -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM Acetate
PH: 5.5
Temperature: 36
uniprot:P00343 [FDP]

Pyruvate + H+ + NADH -> NAD+ + L_Lactate		 (( (Vmax * B) ) / (KmB + B)) buffer: 80 mM Acetate
PH: 4.8
Temperature: 36
- [FDP]

H+ + Pyruvate + NADH -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (S05 + (S ^ n))) buffer: 40 mM Imidazole
PH: 6.2
Temperature: 36
- [FDP]

Pyruvate + H+ + NADH -> NAD+ + L_Lactate		 (( (Vmax * (S ^ n)) ) / (S05 + (S ^ n))) buffer: 40 mM Imidazole
PH: 6.2
Temperature: 36
- [FDP]

Pyruvate + H+ + NADH -> L_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (S05 + (S ^ n))) buffer: 40 mM Imidazole
PH: 6.2
Temperature: 36
- [FDP]

Pyruvate + NADH + H+ -> L_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (S05 + (S ^ n))) buffer: 40 mM Imidazole
PH: 6.2
Temperature: 36
- [FDP]

H+ + NADH + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * B) ) / (KmB + B)) buffer: 80 mM Acetate
PH: 4.8
Temperature: 36
- [FDP]

H+ + NADH + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Imidazole
PH: 7
Temperature: 36
- [FDP]

Pyruvate + NADH + H+ -> NAD+ + L_Lactate		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Imidazole
PH: 6.2
Temperature: 36
- [FDP]

H+ + NADH + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * S) ) / (Km + S)) buffer: 80 mM Acetate
PH: 4.8
Temperature: 36
uniprot:P00343 [FDP]

H+ + NADH + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Imidazole
PH: 7
Temperature: 36
- [FDP]

NADH + H+ + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (S05 + (S ^ n))) buffer: 40 mM Imidazole
PH: 6.2
Temperature: 36
- [FDP]

NADH + Pyruvate + H+ -> L_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (S05 + (S ^ n))) buffer: 40 mM Imidazole
PH: 6.2
Temperature: 36
uniprot:P00343 [FDP]

H+ + NADH + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * S) ) / (Km + S)) buffer: 80 mM Acetate
PH: 5.5
Temperature: 36
uniprot:P00343 [FDP]

Pyruvate + H+ + NADH -> L_Lactate + NAD+		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Imidazole
PH: 6.2
Temperature: 36
uniprot:P00343 [FDP]

Pyruvate + H+ + NADH -> L_Lactate + NAD+		 (( (Vmax * S) ) / (Km + S)) buffer: 80 mM Acetate
PH: 5.5
Temperature: 36
- [FDP]

Pyruvate + NADH + H+ -> L_Lactate + NAD+		 (( (Vmax * (S ^ n)) ) / (S05 + (S ^ n))) buffer: 80 mM Acetate
PH: 4.8
Temperature: 36
uniprot:P00343 [FDP]

NADH + H+ + Pyruvate -> L_Lactate + NAD+		 (( (Vmax * S) ) / (Km + S)) buffer: 80 mM Acetate
PH: 5.5
Temperature: 36
- [FDP]

NADH + H+ + Pyruvate -> NAD+ + L_Lactate		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM Acetate
PH: 4.8
Temperature: 36
- [FDP]

Pyruvate + H+ + NADH -> L_Lactate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM Acetate
PH: 4.8
Temperature: 36
- [L-lactate dehydrogenase(Enzyme) wildtype]

L_Lactate + NAD+ -> H+ + NADH + Pyruvate		 (( (Vmax * S1) ) / (Km + S1)) buffer: 25 mM Tris-maleate
PH: 8.2
Temperature: 30
uniprot:P07195 [L-lactate dehydrogenase(Enzyme) wildtype]

NADH + Pyruvate + H+ -> NAD+ + L_Lactate		 (( (( (E * kcat) ) * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) buffer: 20 mM Bis-Tris, 50 mM KCl
PH: 6
Temperature: 25
uniprot:P07195 [L-lactate dehydrogenase(Enzyme) mutant S163L]

H+ + Pyruvate + NADH -> D_Lactate + NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) buffer: 20 mM Bis-Tris, 50 mM KCl
PH: 6
Temperature: 25
- [Fructose 1,6-bisphosphate]

NADH + H+ + Pyruvate -> NAD+ + L_Lactate		 (( (( (Vmax * (1 - exp((( (0 - kapp) ) * t)))) ) * A) ) / (Ka + A)) buffer: 0.1 M Mes
PH: 6.1
Temperature: 26
- [Fructose 1,6-bisphosphate]

H+ + NADH + Pyruvate -> NAD+ + L_Lactate		 (( (( (Vmax * (1 - exp((( (0 - kapp) ) * t)))) ) * A) ) / (Ka + A)) buffer: 0.1 M Mes
PH: 6.1
Temperature: 26
- [Fructose 1,6-bisphosphate]

Pyruvate + NADH + H+ -> L_Lactate + NAD+		 (( (( (Vmax * (1 - exp((( (0 - kapp) ) * t)))) ) * A) ) / (Ka + A)) buffer: 0.1 M Mes
PH: 6.1
Temperature: 26
- [Fructose 1,6-bisphosphate]

NADH + Pyruvate + H+ -> L_Lactate + NAD+		 (( (( (Vmax * (1 - exp((( (0 - kapp) ) * t)))) ) * A) ) / (Ka + A)) buffer: 0.1 M Mes
PH: 6.1
Temperature: 26
- [Co2+]

H+ + NADH + Pyruvate -> NAD+ + L_Lactate		 (( (( (Vmax * (1 - exp((( (0 - kapp) ) * t)))) ) * A) ) / (Ka + A)) buffer: 0.1 M Mes
PH: 5.5
Temperature: 26
- [Co2+]

Pyruvate + NADH + H+ -> L_Lactate + NAD+		 (( (( (Vmax * (1 - exp((( (0 - kapp) ) * t)))) ) * A) ) / (Ka + A)) buffer: 0.1 M Mes
PH: 5.5
Temperature: 26
- [Co2+]

NADH + H+ + Pyruvate -> NAD+ + L_Lactate		 (( (( (Vmax * (1 - exp((( (0 - kapp) ) * t)))) ) * A) ) / (Ka + A)) buffer: 0.1 M Mes
PH: 5.5
Temperature: 26
- [Co2+]

H+ + NADH + Pyruvate -> L_Lactate + NAD+		 (( (( (Vmax * (1 - exp((( (0 - kapp) ) * t)))) ) * A) ) / (Ka + A)) buffer: 0.1 M Mes
PH: 5.5
Temperature: 26

Pathways

pathway id name
BioCyc:META_P122-PWY heterolactic fermentation
BioCyc:BSUB_PWY-5481 pyruvate fermentation to lactate
BioCyc:GCF_000013425_ANAEROFRUCAT-PWY homolactic fermentation
BioCyc:META_PWY-5031 L-histidine degradation V
BioCyc:META_PWY-5481 pyruvate fermentation to lactate
BioCyc:META_ANAEROFRUCAT-PWY homolactic fermentation
BioCyc:ANTHRA_PWY-5481 pyruvate fermentation to lactate
BioCyc:ANTHRA_PWY0-1317 L-lactaldehyde degradation (aerobic)
BioCyc:GCF_000013425_P41-PWY pyruvate fermentation to acetate and lactate I
BioCyc:GCF_000013425_P122-PWY heterolactic fermentation
BioCyc:META_P41-PWY pyruvate fermentation to acetate and lactate I
BioCyc:META_P124-PWY Bifidobacterium shunt
BioCyc:ANTHRA_P41-PWY pyruvate fermentation to acetate and lactate I
BioCyc:MOUSE_PWY-5481 pyruvate fermentation to lactate
BioCyc:HUMAN_PWY-5481 lactate fermentation (reoxidation of cytosolic NADH)
BioCyc:META_P461-PWY hexitol fermentation to lactate, formate, ethanol and acetate
BioCyc:ANTHRA_PWY-5100 pyruvate fermentation to acetate and lactate II
BioCyc:CORYNE_ANAEROFRUCAT-PWY homolactic fermentation
BioCyc:CORYNE_PWY-5481 pyruvate fermentation to lactate
BioCyc:GCF_000013425_PWY-5481 pyruvate fermentation to lactate
Reactome:R-HSA-9861718 Regulation of pyruvate metabolism
Reactome:R-BTA-9861718 Regulation of pyruvate metabolism
Reactome:R-DRE-9861718 Regulation of pyruvate metabolism
Reactome:R-MMU-9861718 Regulation of pyruvate metabolism
Reactome:R-RNO-9861718 Regulation of pyruvate metabolism
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-DME-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-GGA-1660598 Metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-CEL-70268 Pyruvate metabolism
Reactome:R-DRE-70268 Pyruvate metabolism
Reactome:R-DME-1430728 Metabolism
Reactome:R-GGA-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-HSA-70268 Pyruvate metabolism
Reactome:R-MMU-70268 Pyruvate metabolism
Reactome:R-RNO-70268 Pyruvate metabolism
Reactome:R-CEL-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-GGA-1430728 Metabolism
Reactome:R-GGA-70268 Pyruvate metabolism
Reactome:R-GGA-373920 Pyruvate metabolism
Reactome:R-HSA-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-MMU-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-BTA-70268 Pyruvate metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DME-70268 Pyruvate metabolism
Reactome:R-HSA-1430728 Metabolism
WikiPathways:WP4595 Urea cycle and associated pathways
WikiPathways:WP5166 Glyoxylate metabolism
WikiPathways:WP5298 Dravet syndrome: Scn1a-A1783V point mutation model
WikiPathways:WP5381 Smith-Magenis and Potocki-Lupski syndrome copy number variation
WikiPathways:WP4236 Krebs cycle disorders
WikiPathways:WP5220 Metabolic reprogramming in pancreatic cancer
PathBank:SMP0120734 Malonic Aciduria
PathBank:SMP0120786 Malonyl-CoA Decarboxylase Deficiency
PathBank:SMP0000016 Propanoate Metabolism
PathBank:SMP0000334 Pyruvate Decarboxylase E1 Component Deficiency (PDHE1 Deficiency)
PathBank:SMP0000374 Glycogen Storage Disease Type 1A (GSD1A) or Von Gierke Disease
PathBank:SMP0000560 Phosphoenolpyruvate Carboxykinase Deficiency 1 (PEPCK1)
PathBank:SMP0000722 Cystinosis, Ocular Nonnephropathic
PathBank:SMP0000196 Leigh Syndrome
PathBank:SMP0000201 Methylmalonic Aciduria Due to Cobalamin-Related Disorders
PathBank:SMP0000499 beta-Mercaptolactate-Cysteine Disulfiduria
PathBank:SMP0000559 Pyruvate Kinase Deficiency
PathBank:SMP0000563 Triosephosphate Isomerase Deficiency
PathBank:SMP0087341 Propanoate Metabolism
PathBank:SMP0120748 Methylmalonic Aciduria Due to Cobalamin-Related Disorders
PathBank:SMP0000013 Cysteine Metabolism
PathBank:SMP0000128 Gluconeogenesis
PathBank:SMP0000212 Pyruvate Dehydrogenase Complex Deficiency
PathBank:SMP0000573 Glycogenosis, Type IB
PathBank:SMP0000581 Glycogenosis, Type IA. Von Gierke Disease
PathBank:SMP0000654 Warburg Effect
PathBank:SMP0087248 Propanoate Metabolism
PathBank:SMP0087480 Propanoate Metabolism
PathBank:SMP0000060 Pyruvate Metabolism
PathBank:SMP0000198 Malonic Aciduria
PathBank:SMP0000502 Malonyl-CoA Decarboxylase Deficiency
PathBank:SMP0000558 Primary Hyperoxaluria II, PH2
PathBank:SMP0000562 Fructose-1,6-diphosphatase Deficiency
PathBank:SMP0000574 Glycogenosis, Type IC
PathBank:SMP0002298 Glutaminolysis and Cancer