EC: 1.14.20.6

flavonol synthase (dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:Q96330 [Fe2+]

O2 + Taxifolin + 2_Oxoglutarate -> Succinate + H2O + Quercetin + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 111 mM Sodium acetate, 42 µM Fe(II) sulfate, 2.5 mM Sodium ascorbate
PH: 7
Temperature: 37
uniprot:Q96330 [Fe2+]

2_Oxoglutarate + Taxifolin + O2 -> Quercetin + H2O + Succinate + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 111 mM Sodium acetate, 42 µM Fe(II) sulfate, 2.5 mM Sodium ascorbate
PH: 7
Temperature: 37
uniprot:Q96330 [Fe2+]

2_Oxoglutarate + O2 + Taxifolin -> Quercetin + CO2 + H2O + Succinate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 111 mM Sodium acetate, 42 µM Fe(II) sulfate, 2.5 mM Sodium ascorbate
PH: 7
Temperature: 37
uniprot:Q96330 [Fe2+]

2_Oxoglutarate + Taxifolin + O2 -> Quercetin + H2O + CO2 + Succinate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 111 mM Sodium acetate, 42 µM Fe(II) sulfate, 2.5 mM Sodium ascorbate
PH: 7
Temperature: 37
uniprot:Q96330 [Fe2+]

Taxifolin + O2 + 2_Oxoglutarate -> Quercetin + H2O + Succinate + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 111 mM Sodium acetate, 42 µM Fe(II) sulfate, 2.5 mM Sodium ascorbate
PH: 7
Temperature: 37
uniprot:Q96330 [Fe2+]

2_Oxoglutarate + O2 + Taxifolin -> CO2 + H2O + Quercetin + Succinate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 111 mM Sodium acetate, 42 µM Fe(II) sulfate, 2.5 mM Sodium ascorbate
PH: 7
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY1F-FLAVSYN flavonoid biosynthesis
BioCyc:META_PWY-3101 flavonol biosynthesis
PathBank:SMP0012025 Flavone and Flavonol Biosynthesis