EC: 1.14.18.1

tyrosinase (L-tyrosine,L-dopa:oxygen oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P14679 [monophenol monooxygenase(Enzyme) wildtype]

O2 + L_Tyrosine -> Dopaquinone + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: Phosphate
PH: 7.4
Temperature: 37
uniprot:Q8MIU0 [Cu2+]

O2 + 2 L_Dopa -> 2 L_Dopaquinone + 2 H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 7.2
Temperature: 20
uniprot:P02638 [Cu2+]

L_Dopa + O2 -> L_Dopaquinone + H2O2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 7.2
Temperature: 20
uniprot:Q8MIU0 [Cu2+]

O2 + 2 L_Adrenaline -> 2 H2O + 2 L_Adrenalinequinone		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 7.2
Temperature: 20
uniprot:P02638 [Cu2+]

L_Adrenaline + O2 -> L_Adrenalinequinone + H2O2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 7.2
Temperature: 20
uniprot:P02638 [Cu2+]

O2 + L_Noradrenaline -> L_Noradrenalinequinone + H2O2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 7.2
Temperature: 20
uniprot:Q8MIU0 [Cu2+]

O2 + 2 D_Dopa -> 2 D_Dopaquinone + 2 H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 7.2
Temperature: 20
uniprot:P02638 [Cu2+]

D_Dopa + O2 -> H2O2 + D_Dopaquinone		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 7.2
Temperature: 20
uniprot:Q8MIU0 [Cu2+]

2 L_Noradrenaline + O2 -> 2 H2O + 2 L_Noradrenalinequinone		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris
PH: 7.2
Temperature: 20
uniprot:O42713 [Quercetin]

L_Dopa + O2 -> L_Dopachrome + H2O		 (( (Vmax * A) ) / (Km_app + A)) buffer: 50 mM sodium phosphate
PH: 7
Temperature: 25
uniprot:O42713 [3-Methyl-2-benzothiazolone hydrazone]

O2 + Quercetin -> H2O + Quercetin_o_quinone		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate
PH: 7
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

L_Dopa + O2 -> H2O + L_Dopachrome		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate
PH: 7
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

O2 + Dopaxanthin -> 2 H2O + Dopaxanthinquinone		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM sodium phosphate
PH: 6
Temperature: 25
- [monophenol monooxygenase(Enzyme) wildtype]

2 H2O2 -> O2 + 2 H2O		 (( (k * A) ) * E) buffer: 0.1 M phosphate
PH: 7
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

2 H2O2 -> 2 H2O + O2		 (( (k * A) ) * E) buffer: 0.1 M phosphate
PH: 7
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

4_Cresol + H2O2 -> 4_Methylcatechol + H2O		 (Vmax / (1 + (Km / P))) buffer: 0.5 M borate
PH: 7
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

4_Chlorophenol + H2O2 -> H2O + 4_Chlorocatechol		 (Vmax / (1 + (Km / P))) buffer: 0.5 M borate
PH: 7
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

p_Bromophenol + H2O2 -> H2O + 4_Bromocatechol		 (Vmax / (1 + (Km / P))) buffer: 0.5 M borate
PH: 7
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

H2O2 + p_Fluorophenol -> H2O + 4_Fluorocatechol		 (Vmax / (1 + (Km / P))) buffer: 0.5 M borate
PH: 7
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

Methylparaben + H2O2 -> H2O + Methyl_3,4_dihydroxybenzoate		 (Vmax / (1 + (Km / P))) buffer: 0.5 M borate
PH: 7
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

L_Tyrosine + O2 + L_Dopa -> H2O + L_Dopa + Dopaquinone		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M Na2HPO4-NaH2PO4
PH: 6.8
Temperature: 30
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

O2 + 2 Dopamine -> 2 Dopamine_quinone + 2 H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 2% N,N'-dimethylformamide
PH: 6.8
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

O2 + 2 Catechol -> 2 H2O + 2 1,2_Benzoquinone		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 2% N,N'-dimethylformamide
PH: 6.8
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

O2 + 2 3,4_Dihydroxyphenyl_propionic_acid -> 2 H2O + 2 2_(3,4_Dioxocyclohexa_1,5_dien_1_yl)propionic_acid		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 2% N,N'-dimethylformamide
PH: 6.8
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

2 3,4_Dihydroxyphenylacetate + O2 -> 2 H2O + 2 2_(3,4_Dioxocyclohexa_1,5_dien_1_yl)acetic_acid		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 2% N,N'-dimethylformamide
PH: 6.8
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

O2 + 2 4_Methylcatechol -> 2 4_Methyl_o_benzoquinone + 2 H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 2% N,N'-dimethylformamide
PH: 6.8
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

2 4_tert_Butylcatechol + O2 -> 2 4_tert_Butyl_o_benzoquinone + 2 H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 2% N,N'-dimethylformamide
PH: 6.8
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

O2 + 2 L_Dopa -> 2 H2O + 2 Dopaquinone		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 2% N,N'-dimethylformamide
PH: 6.8
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

4_Hydroxyphenyl_propionic_acid + O2 -> H2O + 3,4_Dihydroxyphenyl_propionic_acid		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 2% N,N'-dimethylformamide
PH: 6.8
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

O2 + Tyramine -> Dopamine + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 2% N,N'-dimethylformamide
PH: 6.8
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

4_Hydroxyphenylacetate + O2 -> 3,4_Dihydroxyphenylacetate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 2% N,N'-dimethylformamide
PH: 6.8
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

O2 + 2 Levisoprenaline -> 2 H2O + 2 1_[1_Hydroxy_2_(propan_2_ylamino)ethyl]_3,4_dioxocyclohexa_1,5_diene		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 2% N,N'-dimethylformamide
PH: 6.8
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

O2 + 4_Hydroxyanisole -> H2O + 3_Methoxycatechol		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 2% N,N'-dimethylformamide
PH: 6.8
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

4_Ethoxyphenol + O2 -> H2O + 3_Ethoxycatechol		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 2% N,N'-dimethylformamide
PH: 6.8
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

O2 + 2 L_alpha_Methyldopa -> 2 L_alpha_Methyldopaquinone + 2 H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 2% N,N'-dimethylformamide
PH: 6.8
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

2 Dopa_methyl_ester + O2 -> 2 H2O + 2 Dopaquinone_methyl_ester		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 2% N,N'-dimethylformamide
PH: 6.8
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

O2 + L_Tyrosine -> H2O + L_Dopa		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 2% N,N'-dimethylformamide
PH: 6.8
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

Phenol + O2 -> H2O + Catechol		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 2% N,N'-dimethylformamide
PH: 6.8
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

O2 + L_alpha_Methyltyrosine -> H2O + L_alpha_Methyldopa		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 2% N,N'-dimethylformamide
PH: 6.8
Temperature: 25
- [trans-Cinnamaldehyde thiosemicarbazone]

2 L_Dopa + O2 -> 2 H2O + 2 L_Dopaquinone		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / Kis))))) buffer: 50 mM Na2HPO4-NaH2PO4, 3.3% Dimethylsulfoxide
PH: 6.8
Temperature: 30
uniprot:O42713 [L-Proline]

Acetaminophen + O2 -> 4_Acetamido_o_benzoquinone + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodiumphosphate
PH: 6.5
Temperature: 25
uniprot:O42713 [monophenol monooxygenase(Enzyme) wildtype]

O2 + Acetaminophen -> 4_Acetamido_o_benzoquinone + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodiumphosphate
PH: 6.5
Temperature: 25
- [Cu2+]

2 L_Dopa + O2 -> 2 H2O + 2 L_Dopaquinone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 30 mM phosphate
PH: 7
Temperature: 25
- [Cu2+]

O2 + L_Tyrosine -> L_Dopaquinone + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 30 mM phosphate
PH: 7
Temperature: 25
- [Cu2+]

O2 + D_Tyrosine -> D_Dopaquinone + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 30 mM phosphate
PH: 7
Temperature: 25
- [Cu2+]

2 D_Dopa + O2 -> 2 H2O + 2 D_Dopaquinone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 30 mM phosphate
PH: 7
Temperature: 25
uniprot:A7BHQ9 [monophenol monooxygenase(Enzyme) wildtype]

O2 + 2 L_Dopa -> 2 Dopaquinone + 2 H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM Tris-HCl
PH: 7.2
Temperature: 25
uniprot:A7BHQ9 [monophenol monooxygenase(Enzyme) wildtype]

O2 + 2 Dopamine -> 2 Dopamine_quinone + 2 H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM Tris-HCl
PH: 7.2
Temperature: 25
uniprot:A7BHQ9 [monophenol monooxygenase(Enzyme) wildtype]

2 3,4_Dihydroxyphenyl_propionic_acid + O2 -> 2 H2O + 2 2_(3,4_Dioxocyclohexa_1,5_dien_1_yl)propionic_acid		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM Tris-HCl
PH: 7.2
Temperature: 25
uniprot:A7BHQ9 [monophenol monooxygenase(Enzyme) wildtype]

O2 + 4_Cresol -> H2O + 4_Methylcatechol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM Tris-HCl
PH: 7.2
Temperature: 25
uniprot:A7BHQ9 [monophenol monooxygenase(Enzyme) wildtype]

2 4_tert_Butylcatechol + O2 -> 2 H2O + 2 4_tert_Butyl_o_benzoquinone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM Tris-HCl
PH: 7.2
Temperature: 25
uniprot:A7BHQ9 [monophenol monooxygenase(Enzyme) wildtype]

4_tert_Butylphenol + O2 -> 4_tert_Butylcatechol + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM Tris-HCl
PH: 7.2
Temperature: 25

Pathways

pathway id name
BioCyc:HUMAN_PWY-6481 L-dopachrome biosynthesis
BioCyc:HUMAN_PWY-6498 eumelanin biosynthesis
BioCyc:META_PWY-6133 (S)-reticuline biosynthesis II
BioCyc:HUMAN_PWY-6133 (S)-reticuline biosynthesis
BioCyc:META_PWY-6481 L-dopa and L-dopachrome biosynthesis
BioCyc:MOUSE_PWY-6481 L-dopachrome biosynthesis
BioCyc:META_PWY-7917 pheomelanin biosynthesis
BioCyc:META_PWY-6498 eumelanin biosynthesis
Reactome:R-HSA-9856651 MITF-M-dependent gene expression
Reactome:R-HSA-9824585 Regulation of MITF-M-dependent genes involved in pigmentation
Reactome:R-HSA-9730414 MITF-M-regulated melanocyte development
Reactome:R-MMU-1430728 Metabolism
Reactome:R-HSA-1266738 Developmental Biology
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-5662702 Melanin biosynthesis
Reactome:R-MMU-5662702 Melanin biosynthesis
WikiPathways:WP3998 Prader-Willi and Angelman syndrome
PathBank:SMP0000070 Riboflavin Metabolism
PathBank:SMP0000190 Hawkinsinuria
PathBank:SMP0120491 Hawkinsinuria
PathBank:SMP0121124 Eumelanin Biosynthesis
PathBank:SMP0000169 Alkaptonuria
PathBank:SMP0000429 Disulfiram Action Pathway
PathBank:SMP0063684 Tyrosine Metabolism
PathBank:SMP0120453 Alkaptonuria
PathBank:SMP0120509 Tyrosinemia Type I
PathBank:SMP0120598 Monoamine Oxidase-A Deficiency (MAO-A)
PathBank:SMP0000218 Tyrosinemia Type I
PathBank:SMP0000533 Monoamine Oxidase-A Deficiency (MAO-A)
PathBank:SMP0120559 Tyrosinemia, Transient, of the Newborn
PathBank:SMP0120563 Dopamine beta-Hydroxylase Deficiency
PathBank:SMP0000006 Tyrosine Metabolism
PathBank:SMP0000494 Tyrosinemia, Transient, of the Newborn
PathBank:SMP0000498 Dopamine beta-Hydroxylase Deficiency