EC: 1.1.1.188

prostaglandin-F synthase ((5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate:NADP+ 11-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

H+ + Prostaglandin_H2 + NADPH -> Prostaglandin_F2alpha + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

H+ + NADPH + Prostaglandin_D2 -> NADP+ + 9alpha,11beta_Prostaglandin_F2		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

Glyceraldehyde + NADPH + H+ -> Glycerol + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

D_Xylose + NADPH + H+ -> Xylitol + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

H+ + NADPH + Prostaglandin_D1 -> Prostaglandin_F1alpha + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

Prostaglandin_D3 + H+ + NADPH -> Prostaglandin_F3alpha + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

H+ + 9,10_Phenanthroquinone + NADH -> NAD+ + Phenanthrene_9,10_diol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

p_Nitrobenzaldehyde + H+ + NADH -> NAD+ + p_Nitrobenzyl_alcohol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

NADH + Phenylglyoxal + H+ -> 2_Hydroxyacetophenone + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

5beta_Androstane_17beta_ol_3_one + H+ + NADPH -> NADP+ + Androstanediol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

H+ + p_Nitrobenzaldehyde + NADPH -> p_Nitrobenzyl_alcohol + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

H+ + NADPH + 9,10_Phenanthroquinone -> Phenanthrene_9,10_diol + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

H+ + Menadione + NADPH -> Menadiol + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

H+ + Cyclohexanone + NADPH -> NADP+ + Cyclohexanol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

NADPH + H+ + 4_Nitroacetophenone -> 1_(4_Nitrophenyl)ethanol + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

Phenylglyoxal + NADPH + H+ -> NADP+ + 2_Hydroxyacetophenone		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

Hydrindantin + H+ + NADPH -> NADP+ + Unknown		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [9,10-Phenanthroquinone]

NADPH + H+ + Prostaglandin_D2 -> 9alpha,11beta_Prostaglandin_F2 + NADP+		 (( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [p-Nitrobenzaldehyde]

H+ + Prostaglandin_D2 + NADPH -> NADP+ + 9alpha,11beta_Prostaglandin_F2		 (( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

NADPH + H+ + Prostaglandin_D2 -> 9alpha,11beta_Prostaglandin_F2 + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

5beta_Androstane_17beta_ol_3_one + NADPH + H+ -> Androstanediol + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

H+ + NADPH + Butanal -> 1_Butanol + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

Ubiquinone_10 + NADPH + H+ -> NADP+ + Ubiquinol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

H+ + Prostaglandin_J2 + NADPH -> NADP+ + Unknown		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

Duroquinone + H+ + NADPH -> Duroquinol + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

H+ + NADPH + Daunorubicin -> Unknown + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

H+ + p_Nitrobenzaldehyde + NADPH -> p_Nitrobenzyl_alcohol + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

Menadione + NADH + H+ -> Menadiol + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

9,10_Phenanthroquinone + H+ + NADPH -> Phenanthrene_9,10_diol + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

NADPH + Menadione + H+ -> NADP+ + Menadiol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

4_Nitroacetophenone + NADH + H+ -> NAD+ + 1_(4_Nitrophenyl)ethanol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

4_Nitroacetophenone + NADPH + H+ -> 1_(4_Nitrophenyl)ethanol + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

NADPH + H+ + Phenylglyoxal -> NADP+ + 2_Hydroxyacetophenone		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

NADH + Hydrindantin + H+ -> Unknown + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

H+ + Hydrindantin + NADPH -> NADP+ + Unknown		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37
uniprot:P52897 [prostaglandin-F synthase(Enzyme) wildtype isoenzyme PGF 2]

H+ + NADH + Prostaglandin_D2 -> 9alpha,11beta_Prostaglandin_F2 + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 5 mM glucose 6-phosphate, 2 U/ml Glucose 6-phosphate dehydrogenase
PH: 6.5
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-7455 allopregnanolone biosynthesis
BioCyc:HUMAN_PWY66-378 androgen biosynthesis
BioCyc:META_PWY66-378 androgen biosynthesis
BioCyc:META_PWY-7455 allopregnanolone biosynthesis
Reactome:R-HSA-5362517 Signaling by Retinoic Acid
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-8978868 Fatty acid metabolism
Reactome:R-HSA-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)
Reactome:R-HSA-9006931 Signaling by Nuclear Receptors
Reactome:R-HSA-5365859 RA biosynthesis pathway
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-2142753 Arachidonate metabolism
Reactome:R-HSA-162582 Signaling Pathways
WikiPathways:WP5280 Glucocorticoid biosynthesis
PathBank:SMP0000083 Acetylsalicylic Acid Action Pathway
PathBank:SMP0000087 Rofecoxib Action Pathway
PathBank:SMP0000094 Sulindac Action Pathway
PathBank:SMP0000104 Indomethacin Action Pathway
PathBank:SMP0000120 Naproxen Action Pathway
PathBank:SMP0000106 Meloxicam Action Pathway
PathBank:SMP0000693 Antrafenine Action Pathway
PathBank:SMP0000697 Flurbiprofen Action Pathway
PathBank:SMP0000701 Phenylbutazone Action Pathway
PathBank:SMP0000705 Tiaprofenic Acid Action Pathway
PathBank:SMP0000709 Salicylic Acid Action Pathway
PathBank:SMP0000075 Arachidonic Acid Metabolism
PathBank:SMP0000086 Ibuprofen Action Pathway
PathBank:SMP0000093 Diclofenac Action Pathway
PathBank:SMP0000098 Ketorolac Action Pathway
PathBank:SMP0000102 Bromfenac Action Pathway
PathBank:SMP0000114 Nabumetone Action Pathway
PathBank:SMP0000692 Antipyrine Action Pathway
PathBank:SMP0000696 Fenoprofen Action Pathway
PathBank:SMP0000700 Lornoxicam Action Pathway
PathBank:SMP0000704 Tolmetin Action Pathway
PathBank:SMP0000708 Salicylate-Sodium Action Pathway
PathBank:SMP0000077 Piroxicam Action Pathway
PathBank:SMP0000084 Etodolac Action Pathway
PathBank:SMP0000109 Mefenamic Acid Action Pathway
PathBank:SMP0000289 Diflunisal Action Pathway
PathBank:SMP0000116 Valdecoxib Action Pathway
PathBank:SMP0000650 Doxorubicin Metabolism Pathway
PathBank:SMP0000694 Carprofen Action Pathway
PathBank:SMP0000698 Magnesium Salicylate Action Pathway
PathBank:SMP0000702 Nepafenac Action Pathway
PathBank:SMP0000706 Tenoxicam Action Pathway
PathBank:SMP0000710 Acetaminophen Action Pathway
PathBank:SMP0000353 Leukotriene C4 Synthesis Deficiency
PathBank:SMP0000085 Ketoprofen Action Pathway
PathBank:SMP0000096 Celecoxib Action Pathway
PathBank:SMP0000101 Suprofen Action Pathway
PathBank:SMP0000113 Oxaprozin Action Pathway
PathBank:SMP0000695 Etoricoxib Action Pathway
PathBank:SMP0000699 Lumiracoxib Action Pathway
PathBank:SMP0000703 Trisalicylate-Choline Action Pathway
PathBank:SMP0000707 Salsalate Action Pathway