chitin degradation III (Serratia)

Vaaje-Kolstad G, Horn SJ, Sørlie M, Eijsink VGH. The chitinolytic machinery of Serratia marcescens – a model system for enzymatic degradation of recalcitrant polysaccharides. The FEBS Journal. 2013 Mar 07;280(13):3028–49. doi: 10.1111/febs.12181.; Ohnuma T, Numata T, Osawa T, Mizuhara M, Lampela O, Juffer AH, Skriver K, Fukamizo T. A class V chitinase from Arabidopsis thaliana: gene responses, enzymatic properties, and crystallographic analysis. Planta. 2011 Jul;234(1):123–37. doi: 10.1007/s00425-011-1390-3. PMID: 21390509.; Hunt DE, Gevers D, Vahora NM, Polz MF. Conservation of the chitin utilization pathway in the Vibrionaceae. Appl Environ Microbiol. 2008 Jan;74(1):44–51. PMID: 17933912; PMCID: PMC2223224.; Young VL, Simpson RM, Ward VK. Characterization of an exochitinase from Epiphyas postvittana nucleopolyhedrovirus (family Baculoviridae). J Gen Virol. 2005 Dec;86(Pt 12):3253–61. doi: 10.1099/vir.0.81262-0. PMID: 16298970.; Tanaka T, Fukui T, Imanaka T. Different Cleavage Specificities of the Dual Catalytic Domains in Chitinase from the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1. Journal of Biological Chemistry. 2001 Sep;276(38):35629–35. doi: 10.1074/jbc.m105919200.

Metabolites

Allosamidine

Formula: C25H42N4O14 (622.2697392)

CAS ID: 103782-08-7

Chitobiose

Formula: C16H28N2O11 (424.16930179999997)

CAS ID: 35061-50-8

Copper

Formula: Cu (62.929599)

CAS ID: 7440-50-8

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

N-Acetyl-beta-D-glucosamine

Formula: C8H15NO6 (221.089933)

CAS ID: 14131-68-1

N-Acetyl-D-glucosamine

Formula: C8H15NO6 (221.089933)

CAS ID: 7512-17-6

Oxygen

Formula: O2 (31.98983)

CAS ID: 7782-44-7



Enzyme

EC Number name full name note
3.2.1.14 chitinase (1->4)-2-acetamido-2-deoxy-beta-D-glucan glycanohydrolase
3.2.1.52 beta-N-acetylhexosaminidase beta-N-acetyl-D-hexosaminide N-acetylhexosaminohydrolase


Proteins

Protein ID name full name
P07254 chiA Chitinase A
P11797 chiB Chitinase B
Q54468 chb Chitobiase