(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered)

Tseng H, Martin CH, Nielsen DR, Prather KLJ. Metabolic Engineering of Escherichia coli for Enhanced Production of ( R )- and ( S )-3-Hydroxybutyrate. Appl Environ Microbiol. 2009 May 15;75(10):3137–45. doi: 10.1128/aem.02667-08.; Leaf TA, Srienc F. Metabolic modeling of polyhydroxybutyrate biosynthesis. Biotechnol Bioeng. 1998 Mar 05;57(5):557–70. doi: 10.1002/(sici)1097-0290(19980305)57:5<557::aid-bit8>3.0.co;2-f. PMID: 10099235.

Metabolites

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

iodoacetamide

Formula: C2H4INO (184.9337644)

CAS ID: 144-48-9

NADPH(4-)

Formula: C21H26N7O17P3 (741.0598016)

CAS ID: 53-57-6

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

NADH(2-)

Formula: C21H27N7O14P2 (663.1091182000001)

CAS ID:

NAD(1-)

Formula: C21H26N7O14P2 (662.1012936000001)

CAS ID: 53-84-9

NADP(3-)

Formula: C21H25N7O17P3 (740.051977)

CAS ID: 53-59-8

Dithionitrobenzoic acid

Formula: C14H6N2O8S2-2 (393.9565596)

CAS ID:



Enzyme

EC Number name full name note
1.1.1.157 3-hydroxybutyryl-CoA dehydrogenase (S)-3-hydroxybutanoyl-CoA:NADP+ oxidoreductase
2.3.1.16 acetyl-CoA C-acyltransferase acyl-CoA:acetyl-CoA C-acyltransferase
2.3.1.9 acetyl-CoA C-acetyltransferase acetyl-CoA:acetyl-CoA C-acetyltransferase
3.1.2.20 acyl-CoA hydrolase acyl-CoA hydrolase


Proteins

Protein ID name full name
P0AGG2 tesB Acyl-CoA thioesterase 2
P14611 phaA Acetyl-CoA acetyltransferase
P45359 CA_C2873 Acetyl-CoA acetyltransferase
P52041 hbd Beta-hydroxybutyryl-CoA dehydrogenase, NAD-dependent
P76461 atoB Acetyl-CoA acetyltransferase
Q0KBP1 bktB Beta-ketothiolase BktB