GDP-mannose biosynthesis

Honghong J, Fuping L, Yu L, Xiaoguang L, Yihan L, Hongbin W, Jing L, Yueting C. Synthesis of GDP-mannose using coupling fermentation of recombinant Escherichia coli. Biotechnol Lett. 2011 Jun;33(6):1145–50. doi: 10.1007/s10529-011-0547-2. PMID: 21293904.; Markovitz A, Sydiskis RJ, Lieberman MM. Genetic and Biochemical Studies on Mannose-Negative Mutants That Are Deficient in Phosphomannose Isomerase in Escherichia coli K-12. J Bacteriol. 1967 Nov;94(5):1492–6. doi: 10.1128/jb.94.5.1492-1496.1967.

Metabolites

D-Glucose 6-phosphate

Formula: C6H13O9P (260.0297178)

CAS ID: 56-73-5

Diphosphate

Formula: H4P2O7 (177.9432294)

CAS ID: 2466-09-3

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

Zinc cation

Formula: Zn (63.929145)

CAS ID: 23713-49-7

5-phospho-D-arabinonohydroxamate

Formula: C5H10NO9P-2 (259.009318)

CAS ID:



Enzyme

EC Number name full name note
2.7.7.13 mannose-1-phosphate guanylyltransferase GTP:alpha-D-mannose-1-phosphate guanylyltransferase
5.3.1.8 mannose-6-phosphate isomerase D-mannose-6-phosphate aldose-ketose-isomerase
5.3.1.9 glucose-6-phosphate isomerase alpha-D-glucose-6-phosphate aldose-ketose-isomerase (configuration-inverting)
5.4.2.8 phosphomannomutase alpha-D-mannose 1,6-phosphomutase


Proteins

Protein ID name full name
P00946 manA Mannose-6-phosphate isomerase
P0A6T1 pgi Glucose-6-phosphate isomerase
P24174 manC Mannose-1-phosphate guanylyltransferase
P24175 manB Phosphomannomutase