lacto-series glycosphingolipids biosynthesis

Kuan C, Chang J, Mansson J, Li J, Pegram C, Fredman P, McLendon RE, Bigner DD. Multiple phenotypic changes in mice after knockout of the B3gnt5gene, encoding Lc3 synthase—a key enzyme in lacto-neolacto ganglioside synthesis. BMC Developmental Biology. 2010 Nov 18;10(1):114. doi: 10.1186/1471-213x-10-114.; Nomura T, Takizawa M, Aoki J, Arai H, Inoue K, Wakisaka E, Yoshizuka N, Imokawa G, Dohmae N, Takio K, Hattori M, Matsuo N. Purification, cDNA cloning, and expression of UDP-Gal: glucosylceramide beta-1,4-galactosyltransferase from rat brain. J Biol Chem. 1998 May 29;273(22):13570–7. doi: 10.1074/jbc.273.22.13570. PMID: 9593693.; Chatterjee S, Ghosh N, Khurana S. Purification of uridine diphosphate-galactose:glucosyl ceramide, beta 1-4 galactosyltransferase from human kidney. Journal of Biological Chemistry. 1992 Apr;267(10):7148–53. doi: 10.1016/s0021-9258(19)50550-6.; Chatterjee S, Castiglione E. UDPgalactose:glucosylceramide ß1 ? 4-galactosyltransferase activity in human proximal tubular cells from normal and familial hypercholesterolemic homozygotes. Biochimica et Biophysica Acta (BBA) - General Subjects. 1987 Jan;923(1):136–42. doi: 10.1016/0304-4165(87)90136-x.

Metabolites

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

UDP-alpha-D-glucose(2-)

Formula: C15H22N2O17P2 (564.0393702)

CAS ID:

UDP(3-)

Formula: C9H11N2O12P2 (400.9787246)

CAS ID: 86527-70-0



Enzyme

EC Number name full name note
2.4.1.-
2.4.1.206 lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide 3-beta-N-acetylglucosaminyltransferase
2.4.1.274 glucosylceramide beta-1,4-galactosyltransferase UDP-galactose:beta-D-glucosyl-(1<->1)-ceramide beta-1,4-galactosyltransferase
2.4.1.344 type 2 galactoside alpha-(1,2)-fucosyltransferase GDP-beta-L-fucose:beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R alpha-(1,2)-L-fucosyltransferase (configuration-inverting)
2.4.1.69 type 1 galactoside alpha-(1,2)-fucosyltransferase GDP-beta-L-fucose:beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R alpha-(1,2)-L-fucosyltransferase (configuration-inverting)
2.4.1.80 ceramide glucosyltransferase UDP-glucose:N-acylsphingosine D-glucosyltransferase
2.4.1.86 N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase UDP-alpha-D-galactose:N-acetyl-beta-D-glucosaminyl-R 3-beta-D-galactosyltransferase
2.4.3.1 beta-galactoside alpha-(2,6)-sialyltransferase CMP-N-acetyl-beta-neuraminate:beta-D-galactoside alpha-(2->6)-N-acetylneuraminyltransferase (configuration-inverting)
2.4.3.9 lactosylceramide alpha-2,3-sialyltransferase CMP-N-acetylneuraminate:beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide alpha-(2->3)-N-acetylneuraminyltransferase


Proteins

Protein ID name full name