vibriobactin biosynthesis

Keating TA, Marshall CG, Walsh CT. Vibriobactin biosynthesis in Vibrio cholerae: VibH is an amide synthase homologous to nonribosomal peptide synthetase condensation domains. Biochemistry. 2000 Dec 19;39(50):15513–21. doi: 10.1021/bi001651a. PMID: 11112537.; Keating TA, Marshall CG, Walsh CT. Reconstitution and characterization of the Vibrio cholerae vibriobactin synthetase from VibB, VibE, VibF, and VibH. Biochemistry. 2000 Dec 19;39(50):15522–30. doi: 10.1021/bi0016523. PMID: 11112538.

Metabolites

Diphosphate

Formula: H4P2O7 (177.9432294)

CAS ID: 2466-09-3

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

pyruvate

Formula: C3H3O3 (87.00821880000001)

CAS ID: 57-60-3

2,3-dihydroxybenzoate

Formula: C7H5O4 (153.018783)

CAS ID:

adenosine 5'-monophosphate(2-)

Formula: C10H12N5O7P (345.0474332)

CAS ID:

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

NADH(2-)

Formula: C21H27N7O14P2 (663.1091182000001)

CAS ID:

NAD(1-)

Formula: C21H26N7O14P2 (662.1012936000001)

CAS ID: 53-84-9



Enzyme

EC Number name full name note
1.3.1.28 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (2S,3S)-2,3-dihydro-2,3-dihydroxybenzoate:NAD+ oxidoreductase
3.3.2.1 isochorismatase isochorismate pyruvate-hydrolase
5.4.4.2 isochorismate synthase isochorismate hydroxymutase
6.2.1.71 2,3-dihydroxybenzoate-[aryl-carrier protein] ligase 2,3-dihydroxybenzoate:[aryl-carrier protein] ligase (AMP-forming)


Proteins

Protein ID name full name
O07898 vibC Vibriobactin-specific isochorismate synthase
O07899 vibE Vibriobactin-specific 2,3-dihydroxybenzoate-AMP ligase
P0C6D3 vibB Vibriobactin-specific isochorismatase
Q56632 vibA Vibriobactin-specific 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase