long chain fatty acid ester synthesis (engineered)

Kalscheuer R, Stölting T, Steinbüchel A. Microdiesel: Escherichia coli engineered for fuel production. Microbiology (Reading). 2006 Sep;152(Pt 9):2529–36. doi: 10.1099/mic.0.29028-0. PMID: 16946248.

Metabolites

Acetaldehyde

Formula: C2H4O (44.0262134)

CAS ID: 75-07-0

CO2

Formula: CO2 (43.98983)

CAS ID: 124-38-9

Diphosphate

Formula: H4P2O7 (177.9432294)

CAS ID: 2466-09-3

Ethanol

Formula: C2H6O (46.0418626)

CAS ID: 64-17-5

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

Ubiquinone-1

Formula: C14H18O4 (250.1205028)

CAS ID: 727-81-1

QH(2)

Formula: C14H20O4 (252.136152)

CAS ID: 52590-98-4

pyruvate

Formula: C3H3O3 (87.00821880000001)

CAS ID: 57-60-3

adenosine 5'-monophosphate(2-)

Formula: C10H12N5O7P (345.0474332)

CAS ID:

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

thiamine(1+) diphosphate(3-)

Formula: C12H16N4O7P2S (422.0214926)

CAS ID:



Enzyme

EC Number name full name note
1.1.1.1 alcohol dehydrogenase alcohol:NAD+ oxidoreductase
2.3.1.20 diacylglycerol O-acyltransferase acyl-CoA:1,2-diacyl-sn-glycerol O-acyltransferase
2.3.1.75 long-chain-alcohol O-fatty-acyltransferase acyl-CoA:long-chain-alcohol O-acyltransferase


Proteins

Protein ID name full name
P0DJA2 adhB Alcohol dehydrogenase 2
Q8GGG1 wax-dgaT O-acyltransferase WSD