esculetin biosynthesis

Yang S, Shim GY, Kim B, Ahn J. Biological synthesis of coumarins in Escherichia coli. Microbial Cell Factories. 2015 May 01;14(1):65. doi: 10.1186/s12934-015-0248-y.; Kai K, Mizutani M, Kawamura N, Yamamoto R, Tamai M, Yamaguchi H, Sakata K, Shimizu B. Scopoletin is biosynthesized viaortho-hydroxylation of feruloyl CoA by a 2-oxoglutarate-dependent dioxygenase inArabidopsis thaliana. The Plant Journal. 2008 Sep;55(6):989–99. doi: 10.1111/j.1365-313x.2008.03568.x.

Metabolites

CO2

Formula: CO2 (43.98983)

CAS ID: 124-38-9

Esculetin

Formula: C9H6O4 (178.0266076)

CAS ID: 305-01-1

Fe2+

Formula: Fe (55.934939)

CAS ID: 15438-31-0

Oxygen

Formula: O2 (31.98983)

CAS ID: 7782-44-7

2-oxoglutarate(2-)

Formula: C5H4O5 (144.00587339999998)

CAS ID: 64-15-3

succinate(2-)

Formula: C4H4O4 (116.01095839999999)

CAS ID: 56-14-4



Enzyme

EC Number name full name note
1.14.11.61 feruloyl-CoA 6-hydroxylase feruloyl-CoA,2-oxoglutarate:oxygen oxidoreductase (6-hydroxylating)
1.14.11.62 trans-4-coumaroyl-CoA 2-hydroxylase (2E)-3-(4-hydroxyphenyl)prop-2-enoyl-CoA,2-oxoglutarate:oxygen oxidoreductase (2-hydroxylating)


Proteins

Protein ID name full name