Major pathway of rRNA processing in the nucleolus and cytosol

McCann KL, Charette JM, Vincent NG, Baserga SJ. A protein interaction map of the LSU processome. Genes Dev. 2015 Apr 15;29(8):862–75. PMID: 25877921; PMCID: PMC4403261.; Henras AK, Plisson-Chastang C, O'Donohue MF, Chakraborty A, Gleizes PE. An overview of pre-ribosomal RNA processing in eukaryotes. Wiley Interdiscip Rev RNA. 2015 Mar;6(2):225–42. PMID: 25346433; PMCID: PMC4361047.; Tafforeau L, Zorbas C, Langhendries JL, Mullineux ST, Stamatopoulou V, Mullier R, Wacheul L, Lafontaine DL. The complexity of human ribosome biogenesis revealed by systematic nucleolar screening of Pre-rRNA processing factors. Mol Cell. 2013 Aug 22;51(4):539–51. doi: 10.1016/j.molcel.2013.08.011. PMID: 23973377.; Popov A, Smirnov E, Kovácik L, Raška O, Hagen G, Stixová L, Raška I. Duration of the first steps of the human rRNA processing. Nucleus. 2013 Mar;4(2):134–41. PMID: 23412654; PMCID: PMC3621745.; Mullineux ST, Lafontaine DL. Mapping the cleavage sites on mammalian pre-rRNAs: where do we stand? Biochimie. 2012 Jul;94(7):1521–32. doi: 10.1016/j.biochi.2012.02.001. PMID: 22342225.; Phipps KR, Charette J, Baserga SJ. The small subunit processome in ribosome biogenesis—progress and prospects. Wiley Interdiscip Rev RNA. 2011 Jan;2(1):1–21. PMID: 21318072; PMCID: PMC3035417.; Hernandez-Verdun D, Roussel P, Thiry M, Sirri V, Lafontaine DL. The nucleolus: structure/function relationship in RNA metabolism. Wiley Interdiscip Rev RNA. 2010 Nov;1(3):415–31. doi: 10.1002/wrna.39. PMID: 21956940.; Grandi P, Rybin V, Bassler J, Petfalski E, Strauss D, Marzioch M, Schäfer T, Kuster B, Tschochner H, Tollervey D, Gavin AC, Hurt E. 90S pre-ribosomes include the 35S pre-rRNA, the U3 snoRNP, and 40S subunit processing factors but predominantly lack 60S synthesis factors. Mol Cell. 2002 Jul;10(1):105–15. doi: 10.1016/s1097-2765(02)00579-8. PMID: 12150911.; Dragon F, Gallagher JE, Compagnone-Post PA, Mitchell BM, Porwancher KA, Wehner KA, Wormsley S, Settlage RE, Shabanowitz J, Osheim Y, Beyer AL, Hunt DF, Baserga SJ. A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis. Nature. 2002 Jun 27;417(6892):967–70. PMID: 12068309; PMCID: PMC11487672.; Stanek D, Koberna K, Pliss A, Malínský J, Masata M, Vecerová J, Risueño MC, Raska I. Non-isotopic mapping of ribosomal RNA synthesis and processing in the nucleolus. Chromosoma. 2001 Dec;110(7):460–70. doi: 10.1007/s00412-001-0172-2. PMID: 11862453.

Metabolites



Enzyme

EC Number name full name note
2.1.1.-
2.7.11.1 non-specific serine/threonine protein kinase ATP:protein phosphotransferase (non-specific)
3.1.26.5 ribonuclease P
3.6.4.13 RNA helicase


Proteins

Protein ID name full name
O14232 mtr4 ATP-dependent RNA helicase mtr4
O42650 rio1 Serine/threonine-protein kinase rio1
O42957 ulp1 Ubiquitin-like-specific protease 1
P40235 hhp1 Casein kinase I homolog hhp1
P87120 RNP3_SCHPO Probable ribonuclease P protein subunit 3
Q09775 rok1 ATP-dependent RNA helicase rok1
Q10107 mra1 Ribosomal RNA small subunit methyltransferase mra1
Q10146 rrp6 Exosome complex exonuclease rrp6
Q10162 bud23 18S rRNA (guanine-N(7))-methyltransferase bud23
Q9HDY4 YK16_SCHPO Putative ATP-dependent RNA helicase PB1A10.06c
Q9HGM5 dbp8 ATP-dependent RNA helicase dbp8
Q9P6N8 rrp3 ATP-dependent rRNA helicase rrp3
Q9P7W5 rio2 Serine/threonine-protein kinase rio2
Q9UTK0 nob1 20S-pre-rRNA D-site endonuclease nob1
Q9UU96 grc3 Polynucleotide 5'-hydroxyl-kinase grc3
Q9Y813 rpr2 Ribonuclease P protein subunit rpr2