Synthesis of glycosylphosphatidylinositol (GPI)

Shishioh N, Hong Y, Ohishi K, Ashida H, Maeda Y, Kinoshita T. GPI7 Is the Second Partner of PIG-F and Involved in Modification of Glycosylphosphatidylinositol. Journal of Biological Chemistry. 2005 Mar;280(10):9728–34. doi: 10.1074/jbc.m413755200.; Taron BW, Colussi PA, Wiedman JM, Orlean P, Taron CH. Human Smp3p Adds a Fourth Mannose to Yeast and Human Glycosylphosphatidylinositol Precursors in Vivo. Journal of Biological Chemistry. 2004 Aug;279(34):36083–92. doi: 10.1074/jbc.m405081200.; Kinoshita T, Inoue N. Dissecting and manipulating the pathway for glycosylphos-phatidylinositol-anchor biosynthesis. Current Opinion in Chemical Biology. 2000 Dec;4(6):632–8. doi: 10.1016/s1367-5931(00)00151-4.

Metabolites

Acetate

Formula: C2H4O2 (60.0211284)

CAS ID: 64-19-7

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

UDP(3-)

Formula: C9H11N2O12P2 (400.9787246)

CAS ID: 86527-70-0



Enzyme

EC Number name full name note
2.-.-.-
2.4.1.198 phosphatidylinositol N-acetylglucosaminyltransferase UDP-N-acetyl-D-glucosamine:1-phosphatidyl-1D-myo-inositol 6-(N-acetyl-alpha-D-glucosaminyl)transferase
3.5.1.89 N-acetylglucosaminylphosphatidylinositol deacetylase 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol acetylhydrolase


Proteins

Protein ID name full name