Phosphate bond hydrolysis by NUDT proteins

Takagi Y, Setoyama D, Ito R, Kamiya H, Yamagata Y, Sekiguchi M. Human MTH3 (NUDT18) protein hydrolyzes oxidized forms of guanosine and deoxyguanosine diphosphates: comparison with MTH1 and MTH2. J Biol Chem. 2012 Jun 15;287(25):21541–9. PMID: 22556419; PMCID: PMC3375575.; Iyama T, Abolhassani N, Tsuchimoto D, Nonaka M, Nakabeppu Y. NUDT16 is a (deoxy)inosine diphosphatase, and its deficiency induces accumulation of single-strand breaks in nuclear DNA and growth arrest. Nucleic Acids Res. 2010 Aug;38(14):4834–43. PMID: 20385596; PMCID: PMC2919730.; McLennan AG. The Nudix hydrolase superfamily. Cellular and Molecular Life Sciences. 2005 Dec 27;63(2):123–43. doi: 10.1007/s00018-005-5386-7.; Mildvan AS, Xia Z, Azurmendi HF, Saraswat V, Legler PM, Massiah MA, Gabelli SB, Bianchet MA, Kang L-, Amzel LM. Structures and mechanisms of Nudix hydrolases. Archives of Biochemistry and Biophysics. 2005 Jan;433(1):129–43. doi: 10.1016/j.abb.2004.08.017.; Shen BW, Perraud AL, Scharenberg A, Stoddard BL. The crystal structure and mutational analysis of human NUDT9. J Mol Biol. 2003 Sep 12;332(2):385–98. doi: 10.1016/s0022-2836(03)00954-9. PMID: 12948489.; Perraud A, Shen B, Dunn CA, Rippe K, Smith MK, Bessman MJ, Stoddard BL, Scharenberg AM. NUDT9, a Member of the Nudix Hydrolase Family, Is an Evolutionarily Conserved Mitochondrial ADP-ribose Pyrophosphatase. Journal of Biological Chemistry. 2003 Jan;278(3):1794–801. doi: 10.1074/jbc.m205601200.

Metabolites

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

Magnesium cation

Formula: Mg (23.98505)

CAS ID: 22537-22-0

Manganese(2+)

Formula: Mn (54.938046)

CAS ID: 7439-96-5



Enzyme

EC Number name full name note
3.6.1.-
2.7.7.96 ADP-D-ribose pyrophosphorylase ATP:D-ribose 5-phosphate adenylyltransferase
3.6.1.13 ADP-ribose diphosphatase ADP-D-ribose ribophosphohydrolase
3.6.1.16 CDP-glycerol diphosphatase CDP-glycerol phosphoglycerohydrolase
3.6.1.53 Mn2+-dependent ADP-ribose/CDP-alcohol diphosphatase CDP-choline phosphohydrolase
3.6.1.56 2-hydroxy-dATP diphosphatase 2-hydroxy-dATP diphosphohydrolase
3.6.1.58 8-oxo-dGDP phosphatase 8-oxo-dGDP phosphohydrolase
3.6.1.62 5'-(N7-methylguanosine 5'-triphospho)-[mRNA] hydrolase 5'-(N7-methylguanosine 5'-triphospho)-[mRNA] N7-methylguanosine-5'-diphosphate phosphohydrolase
3.6.1.64 inosine diphosphate phosphatase inosine diphosphate phosphatase
3.6.1.68 geranyl diphosphate phosphohydrolase geranyl-diphosphate phosphohydrolase
3.6.1.76 prenyl-diphosphate phosphatase prenyl diphosphate/3-methylbut-3-en-1-yl diphosphate phosphohydrolase
3.6.1.9 nucleotide diphosphatase dinucleotide nucleotidohydrolase


Proteins

Protein ID name full name