GTP hydrolysis and joining of the 60S ribosomal subunit

Asano K, Clayton J, Shalev A, Hinnebusch AG. A multifactor complex of eukaryotic initiation factors, eIF1, eIF2, eIF3, eIF5, and initiator tRNAMet is an important translation initiation intermediate in vivo. Genes Dev. 2000 Oct 01;14(19):2534–46. doi: 10.1101/gad.831800.; Pestova TV, Lomakin IB, Lee JH, Choi SK, Dever TE, Hellen CU. The joining of ribosomal subunits in eukaryotes requires eIF5B. Nature. 2000 Jan 20;403(6767):332–5. doi: 10.1038/35002118. PMID: 10659855.; Chakrabarti A, Maitra U. Function of eukaryotic initiation factor 5 in the formation of an 80 S ribosomal polypeptide chain initiation complex. Journal of Biological Chemistry. 1991 Jul;266(21):14039–45. doi: 10.1016/s0021-9258(18)92807-3.; Peterson DT, Merrick WC, Safer B. Binding and release of radiolabeled eukaryotic initiation factors 2 and 3 during 80 S initiation complex formation. Journal of Biological Chemistry. 1979 Apr;254(7):2509–16. doi: 10.1016/s0021-9258(17)30251-x.; Benne R, Hershey JW. The mechanism of action of protein synthesis initiation factors from rabbit reticulocytes. Journal of Biological Chemistry. 1978 May;253(9):3078–87. doi: 10.1016/s0021-9258(17)40805-2.; Schreier MH, Erni B, Staehelin T. Initiation of mammalian protein synthesis. I. Purification and characterization of seven initiation factors. J Mol Biol. 1977 Nov;116(4):727–53. doi: 10.1016/0022-2836(77)90268-6. PMID: 592398.; Trachsel H, Erni B, Schreier MH, Staehelin T. Initiation of mammalian protein synthesis. II. The assembly of the initiation complex with purified initiation factors. J Mol Biol. 1977 Nov;116(4):755–67. doi: 10.1016/0022-2836(77)90269-8. PMID: 592399.; Merrick WC, Kemper WM, Anderson WF. Purification and characterization of homogeneous initiation factor M2A from rabbit reticulocytes. Journal of Biological Chemistry. 1975 Jul;250(14):5556–62. doi: 10.1016/s0021-9258(19)41216-7.

Metabolites

GTP(4-)

Formula: C10H12N5O14P3 (518.9593642)

CAS ID: 86527-72-2



Enzyme

EC Number name full name note
3.4.19.12 ubiquitinyl hydrolase 1
3.6.4.13 RNA helicase
3.6.5.3 protein-synthesizing GTPase GTP phosphohydrolase (mRNA-translation-assisting)
4.2.99.18 DNA-(apurinic or apyrimidinic site) lyase DNA-(apurinic or apyrimidinic site) 5'-phosphomonoester-lyase


Proteins

Protein ID name full name