Enterobacterial factors antagonize host defense

Hou Y, Zeng H, Li Z, Feng N, Meng F, Xu Y, Li L, Shao F, Ding J. Structural mechanisms of calmodulin activation of Shigella effector OspC3 to ADP-riboxanate caspase-4/11 and block pyroptosis. Nat Struct Mol Biol. 2023 Mar;30(3):261–72. doi: 10.1038/s41594-022-00888-3. PMID: 36624349.; Li Z, Liu W, Fu J, Cheng S, Xu Y, Wang Z, Liu X, Shi X, Liu Y, Qi X, Liu X, Ding J, Shao F. Shigella evades pyroptosis by arginine ADP-riboxanation of caspase-11. Nature. 2021 Nov;599(7884):290–5. doi: 10.1038/s41586-021-04020-1. PMID: 34671164.; Song T, Li K, Zhou W, Zhou J, Jin Y, Dai H, Xu T, Hu M, Ren H, Yue J, Liang L. A Type III Effector NleF from EHEC Inhibits Epithelial Inflammatory Cell Death by Targeting Caspase-4. BioMed Research International. 2017;2017():1–11. doi: 10.1155/2017/4101745.; Pallett MA, Crepin VF, Serafini N, Habibzay M, Kotik O, Sanchez-Garrido J, Di Santo JP, Shenoy AR, Berger CN, Frankel G. Bacterial virulence factor inhibits caspase-4/11 activation in intestinal epithelial cells. Mucosal Immunol. 2017 May;10(3):602–12. PMID: 27624779; PMCID: PMC5159625.; Kobayashi T, Ogawa M, Sanada T, Mimuro H, Kim M, Ashida H, Akakura R, Yoshida M, Kawalec M, Reichhart JM, Mizushima T, Sasakawa C. The Shigella OspC3 effector inhibits caspase-4, antagonizes inflammatory cell death, and promotes epithelial infection. Cell Host Microbe. 2013 May 15;13(5):570–83. doi: 10.1016/j.chom.2013.04.012. PMID: 23684308.; Blasche S, Mörtl M, Steuber H, Siszler G, Nisa S, Schwarz F, Lavrik I, Gronewold TMA, Maskos K, Donnenberg MS, Ullmann D, Uetz P, Kögl M. The E. coli Effector Protein NleF Is a Caspase Inhibitor. PLoS ONE. 2013 Mar 14;8(3):e58937. doi: 10.1371/journal.pone.0058937.

Metabolites

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

Nicotinamide

Formula: C6H6N2O (122.0480106)

CAS ID: 98-92-0

NAD(1-)

Formula: C21H26N7O14P2 (662.1012936000001)

CAS ID: 53-84-9



Enzyme

EC Number name full name note
4.3.99.-
2.3.2.23 E2 ubiquitin-conjugating enzyme S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine:[E2 ubiquitin-conjugating enzyme] ubiquitinyl transferase
2.3.2.24 (E3-independent) E2 ubiquitin-conjugating enzyme S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine:L-lysine ubiquitinyl transferase ([E3 ubiquitin transferase]-independent)
2.3.2.27 RING-type E3 ubiquitin transferase [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine:[acceptor protein] ubiquitin transferase (isopeptide bond-forming; RING-type)
3.4.22.57 caspase-4


Proteins

Protein ID name full name
A0A0H2US87 ospC3 Arginine ADP-riboxanase OspC3
Q8VSC3 ipaH9.8 E3 ubiquitin-protein ligase ipaH9.8