hyaluronan degradation

Maruyama Y, Nakamichi Y, Itoh T, Mikami B, Hashimoto W, Murata K. Substrate Specificity of Streptococcal Unsaturated Glucuronyl Hydrolases for Sulfated Glycosaminoglycan. Journal of Biological Chemistry. 2009 Jul;284(27):18059–69. doi: 10.1074/jbc.m109.005660.; Itoh T, Hashimoto W, Mikami B, Murata K. Substrate recognition by unsaturated glucuronyl hydrolase from Bacillus sp. GL1. Biochemical and Biophysical Research Communications. 2006 May;344(1):253–62. doi: 10.1016/j.bbrc.2006.03.141.; Itoh T, Akao S, Hashimoto W, Mikami B, Murata K. Crystal Structure of Unsaturated Glucuronyl Hydrolase, Responsible for the Degradation of Glycosaminoglycan, from Bacillus sp. GL1 at 1.8 Å Resolution. Journal of Biological Chemistry. 2004 Jul;279(30):31804–12. doi: 10.1074/jbc.m403288200.; Hashimoto W, Kobayashi E, Nankai H, Sato N, Miya T, Kawai S, Murata K. Unsaturated Glucuronyl Hydrolase of Bacillus sp. GL1: Novel Enzyme Prerequisite for Metabolism of Unsaturated Oligosaccharides Produced by Polysaccharide Lyases. Archives of Biochemistry and Biophysics. 1999 Aug;368(2):367–74. doi: 10.1006/abbi.1999.1305.; Dietrich CP, Silva ME, Michelacci YM. Sequential degradation of heparin in Flavobacterium heparinum. Purification and properties of five enzymes involved in heparin degradation. J Biol Chem. 1973 Sep 25;248(18):6408–15. PMID: 4730325.

Metabolites

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

N-Acetyl-D-glucosamine

Formula: C8H15NO6 (221.089933)

CAS ID: 7512-17-6



Enzyme

EC Number name full name note
3.2.1.180 unsaturated chondroitin disaccharide hydrolase beta-D-4-deoxy-DELTA4-GlcAp-(1->3)-beta-D-GalNAc6S hydrolase
4.2.2.1 hyaluronate lyase hyaluronate lyase


Proteins

Protein ID name full name
Q54873 SP_0314 hyaluronidase
Q8E372 UCDH_STRA3 Unsaturated chondroitin disaccharide hydrolase