brassinosteroid biosynthesis III

Nomura T, Jager CE, Kitasaka Y, Takeuchi K, Fukami M, Yoneyama K, Matsushita Y, Nyunoya H, Takatsuto S, Fujioka S, Smith JJ, Kerckhoffs LH, Reid JB, Yokota T. Brassinosteroid deficiency due to truncated steroid 5alpha-reductase causes dwarfism in the lk mutant of pea. Plant Physiol. 2004 Aug;135(4):2220–9. PMID: 15286289; PMCID: PMC520792.; Finsterbusch A, Lindemann P, Grimm R, Eckerskorn C, Luckner M. Delta(5)-3beta-hydroxysteroid dehydrogenase from Digitalis lanata Ehrh. - a multifunctional enzyme in steroid metabolism? Planta. 1999 Oct;209(4):478–86. doi: 10.1007/s004250050751. PMID: 10550629.; Noguchi T, Fujioka S, Takatsuto S, Sakurai A, Yoshida S, Li J, Chory J. Arabidopsis det2 Is Defective in the Conversion of (24R)-24-Methylcholest-4-En-3-One to (24R)-24-Methyl-5a-Cholestan-3-One in Brassinosteroid Biosynthesis1. 1999 Jul 01;120(3):833–40. doi: 10.1104/pp.120.3.833.; Lachance Y, Luu-The V, Labrie C, Simard J, Dumont M, de Launoit Y, Guérin S, Leblanc G, Labrie F. Characterization of human 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase gene and its expression in mammalian cells. Journal of Biological Chemistry. 1990 Nov;265(33):20469–75. doi: 10.1016/s0021-9258(17)30528-8.; TALALAY P, WANG VS. Enzymic isomerization of delta5-3-ketosteroids. Biochim Biophys Acta. 1955 Oct;18(2):300–1. doi: 10.1016/0006-3002(55)90079-2. PMID: 13276386.

Metabolites

5alpha-Campestan-3-one

Formula: C28H48O (400.37049579999996)

CAS ID: 27212-88-0

Campest-4-en-3-one

Formula: C28H46O (398.3548466)

CAS ID: 22260-46-4

Campesterol

Formula: C28H48O (400.37049579999996)

CAS ID: 474-62-4

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

NADPH(4-)

Formula: C21H26N7O17P3 (741.0598016)

CAS ID: 53-57-6

NAD(1-)

Formula: C21H26N7O14P2 (662.1012936000001)

CAS ID: 53-84-9

NADP(3-)

Formula: C21H25N7O17P3 (740.051977)

CAS ID: 53-59-8



Enzyme

EC Number name full name note
1.3.1.-
1.3.1.22 3-oxo-5alpha-steroid 4-dehydrogenase (NADP+) 3-oxo-5alpha-steroid:NADP+ DELTA4-oxidoreductase


Proteins

Protein ID name full name