- [K+-transporting ATPase(Enzyme) wildtype]
K+ + ATP + H2O -> ADP + Phosphate + K+ |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 10 mM Hepes, 5 mM MgCl2, 5% (v/v) glycerol, 2% (w/v) Triton X-100, 1 mM mercaptoethanol, 1 U/ml pyruvate kinase, 0.4 mM K2ATP, 0.2 mM NADH, 0.5 mM phosphoenolpyruvate, 0.5 mg/ml asolectin
PH: 7.5
Temperature: 37 |
- [K+-transporting ATPase(Enzyme) wildtype]
K+ + H2O + ATP -> Phosphate + K+ + ADP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 10 mM Na-borate, 5% glycerol, 5 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 mM EDTA, 0.5 mg/ml soybean phospholipids, 0.5 mM phosphoenolpyruvate, 0.2 mM NADH, 1 U/ml lactate dehydrogenase, 1 U/ml pyruvate kinase
PH: 7.5
Temperature: 37 |