EC: 6.1.1.20

phenylalanine-tRNA ligase (L-phenylalanine:tRNAPhe ligase (AMP-forming))

enzyme lambda metabolic reaction experiment
- [phenylalanine---tRNA ligase(Enzyme) wildtype]

tRNA(Phe) + ATP + L_Phenylalanine -> L_Phenylalanyl_tRNA(Phe) + Diphosphate + AMP		 (( (Vmax * A) ) / (Km + A)) buffer: 180 mM HEPES, 20 mM Magnesium acetate, 10 mM Glutathione, 1% Dimethyl sulfoxide, 2 mM CTP
PH: 7.6
Temperature: 22

Pathways

pathway id name
BioCyc:VCHO_TRNA-CHARGING-PWY tRNA charging
BioCyc:ANTHRA_TRNA-CHARGING-PWY tRNA charging
BioCyc:CAULONA1000_TRNA-CHARGING-PWY tRNA charging pathway
BioCyc:BTHE_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECO_TRNA-CHARGING-PWY tRNA charging
BioCyc:MTBH37RV_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOO157_TRNA-CHARGING-PWY tRNA charging
BioCyc:SYNEL_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOL316407_TRNA-CHARGING-PWY tRNA charging
BioCyc:MTBCDC1551_TRNA-CHARGING-PWY tRNA charging
BioCyc:HPY_TRNA-CHARGING-PWY tRNA charging
BioCyc:CAULO_TRNA-CHARGING-PWY tRNA charging
BioCyc:AGRO_TRNA-CHARGING-PWY tRNA charging
BioCyc:BSUB_TRNA-CHARGING-PWY tRNA charging
BioCyc:GCF_000013425_TRNA-CHARGING-PWY tRNA charging
BioCyc:HUMAN_TRNA-CHARGING-PWY tRNA charging
BioCyc:META_TRNA-CHARGING-PWY tRNA charging
BioCyc:YEAST_TRNA-CHARGING-PWY tRNA charging
BioCyc:MOUSE_TRNA-CHARGING-PWY tRNA charging pathway
BioCyc:CORYNE_TRNA-CHARGING-PWY tRNA charging
PathBank:SMP0000820 tRNA Charging
PathBank:SMP0000824 tRNA Charging 2
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-379726 Mitochondrial tRNA aminoacylation
Reactome:R-HSA-379716 Cytosolic tRNA aminoacylation
Reactome:R-HSA-72766 Translation
Reactome:R-HSA-379724 tRNA Aminoacylation
PathBank:SMP0120540 Phenylketonuria
PathBank:SMP0000369 Tyrosinemia Type 2 (or Richner-Hanhart Syndrome)
PathBank:SMP0120733 Tyrosinemia Type 2 (or Richner-Hanhart Syndrome)
PathBank:SMP0120511 Tyrosinemia Type 3 (TYRO3)
PathBank:SMP0121283 tRNA Charging 2
PathBank:SMP0000206 Phenylketonuria
PathBank:SMP0087313 Phenylalanine and Tyrosine Metabolism
PathBank:SMP0120760 Phenylketonuria
PathBank:SMP0120513 Tyrosinemia Type 2 (or Richner-Hanhart Syndrome)
PathBank:SMP0121281 tRNA Charging
PathBank:SMP0000370 Tyrosinemia Type 3 (TYRO3)
PathBank:SMP0120731 Tyrosinemia Type 3 (TYRO3)
PathBank:SMP0111876 Protein Synthesis: Phenylalanine
PathBank:SMP0119319 Protein Synthesis: Phenylalanine
PathBank:SMP0119475 Protein Synthesis: Phenylalanine
PathBank:SMP0000008 Phenylalanine and Tyrosine Metabolism
PathBank:SMP0063649 Phenylalanine and Tyrosine Metabolism