uniprot:Q9FUZ2 [peptide deformylase(Enzyme) wildtype partially proteolysed]
N_Formyl_Met_Leu_p_nitroanilide + H2O -> Met_Leu_p_nitroanilide + Formate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Potassium phosphate, 100 µM EGTA
PH: 7
Temperature: 23 |
uniprot:Q9FUZ2 [peptide deformylase(Enzyme) wildtype N- and C-terminal truncated]
N_Formyl_Met_Leu_p_nitroanilide + H2O -> Met_Leu_p_nitroanilide + Formate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Potassium phosphate, 100 µM EGTA
PH: 7
Temperature: 23 |
uniprot:Q9FUZ2 [peptide deformylase(Enzyme) wildtype]
H2O + N_Formyl_Met_Leu_p_nitroanilide -> Met_Leu_p_nitroanilide + Formate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Potassium phosphate, 100 µM EGTA
PH: 7
Temperature: 23 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) wildtype AtPDF1Adelta1-67]
H2O + N_Formyl_Met_Leu_Phe -> Formate + Met_Leu_Phe |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA, 4% methanol
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) wildtype AtPDF1Adelta1-67]
N_Formyl_Met_Leu_Glu + H2O -> Met_Leu_Glu + Formate |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) wildtype AtPDF1Adelta1-67]
N_Formyl_Met_Ser_Asn_Glu + H2O -> Met_Ser_Asn_Glu + Formate |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) wildtype AtPDF1Adelta1-67]
H2O + N_Formyl_Nle_Leu_NH2 -> Formate + Nle_Leu_NH2 |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA, 4 % methanol
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) wildtype AtPDF1Adelta1-67]
N_Formyl_Nle_NH(CH3) + H2O -> Formate + Nle_NH(CH3) |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) wildtype AtPDF1Adelta1-67]
N_Formyl_Met_Ala_OCH3 + H2O -> Formate + Met_Ala_OCH3 |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) wildtype AtPDF1Adelta1-67]
N_Formyl_Met_Ala_O_ + H2O -> Met_Ala_O_ + Formate |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) wildtype AtPDF1Adelta1-67]
H2O + N_Formyl_Nle_NH2 -> Formate + Nle_NH2 |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FUZ2 [Ni2+]
N_Formyl_Nle_Ala_Ser + H2O -> Formate + Nle_Ala_Ser |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FUZ2 [Fe2+]
Formyl_L_methionyl_peptide + H2O -> Formate + Methionyl_peptide |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA, and 2 mM Tris(2-carboxyethyl)-phosphine as stabilizing compound
PH: 7.5
Temperature: 37 |
uniprot:Q9FUZ0 [peptide deformylase(Enzyme) wildtype LePDF1Adelta1-86]
Formyl_L_methionyl_peptide + H2O -> Methionyl_peptide + Formate |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) wildtype AtPDF1Adelta1-67]
H2O + Formyl_L_methionyl_peptide -> Formate + Methionyl_peptide |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:P0A6K3 [peptide deformylase(Enzyme) wildtype Zn-EcPDF1]
H2O + Formyl_L_methionyl_peptide -> Methionyl_peptide + Formate |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA, stabilizing compounds: 1 mM NiCL2 or 200 mg/ml catalase.
PH: 7.5
Temperature: 37 |
uniprot:Q9FUZ2 [Ni2+]
Formyl_L_methionyl_peptide + H2O -> Methionyl_peptide + Formate |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) wildtype AtPDF1Adelta1-67]
N_Formyl_Phe_Ala_Ser + H2O -> Phe_Ala_Ser + Formate |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) wildtype AtPDF1Adelta1-67]
N_Formyl_Nle_Asp_NH2 + H2O -> Formate + Nle_Asp_NH2 |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) wildtype AtPDF1Adelta1-67]
N_Formyl_Nle_Gly_NH2 + H2O -> Formate + Nle_Gly_NH2 |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) wildtype AtPDF1Adelta1-67]
H2O + N_Formyl_Nle_Ser_NH2 -> Nle_Ser_NH2 + Formate |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) wildtype AtPDF1Adelta1-67]
H2O + N_Formyl_Nle_Arg_NH2 -> Formate + Nle_Arg_NH2 |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) wildtype AtPDF1Adelta1-67]
N_Formyl_Nva_Ala_Ser + H2O -> Nva_Ala_Ser + Formate |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) wildtype AtPDF1Adelta1-67]
N_Formyl_Nle_Ala_Ser + H2O -> Nle_Ala_Ser + Formate |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FUZ2 [Ni2+]
N_Formyl_Met_Leu_Glu + H2O -> Met_Leu_Glu + Formate |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FUZ2 [Ni2+]
H2O + N_Formyl_Met_Ser_Asn_Glu -> Met_Ser_Asn_Glu + Formate |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FUZ2 [Ni2+]
N_Formyl_Met_Ala_OCH3 + H2O -> Formate + Met_Ala_OCH3 |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FUZ2 [Ni2+]
H2O + N_Formyl_Nle_Arg_NH2 -> Formate + Nle_Arg_NH2 |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FUZ2 [Ni2+]
H2O + N_Formyl_Nle_Ser_NH2 -> Nle_Ser_NH2 + Formate |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FUZ2 [Ni2+]
H2O + N_Formyl_Nle_Gly_NH2 -> Nle_Gly_NH2 + Formate |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FUZ2 [Ni2+]
H2O + N_Formyl_Nle_Asp_NH2 -> Nle_Asp_NH2 + Formate |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FUZ2 [Ni2+]
N_Formyl_Met_Ala_O_ + H2O -> Formate + Met_Ala_O_ |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FUZ2 [Ni2+]
N_Formyl_Nle_Ala_NH2 + H2O -> Nle_Ala_NH2 + Formate |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 mM Hepes, 12 mM NAD+, 4.5 units/ml yeast formate dehydrogenase, 200 µg/ml BSA
PH: 7.5
Temperature: 37 |
uniprot:Q9FUZ2 [peptide deformylase(Enzyme) wildtype DEF2]
N_Formyl_Met_Ala_Ser + H2O -> Met_Ala_Ser + Formate |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 MES, 50 mM bis-tris propane, 12.0 mM beta-NAD+, 1.2 unit formate dehydrogenase
PH: 8
Temperature: 25 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) wildtype DEF1]
N_Formyl_Met_Ala_Ser + H2O -> Formate + Met_Ala_Ser |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 MES, 50 mM bis-tris propane, 12.0 mM beta-NAD+, 1.2 unit formate dehydrogenase
PH: 8
Temperature: 25 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) wildtype DEF1]
N_Formyl_Met_Leu_p_nitroanilide + H2O -> Met_Leu_p_nitroanilide + Formate |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 MES, 50 mM bis-tris propane, 200 µM NiSO4, 1.0 unit Aeromonas proteolytica aminopeptidase
PH: 8
Temperature: 25 |
uniprot:Q9FUZ2 [peptide deformylase(Enzyme) wildtype DEF2]
N_Formyl_Met_Leu_p_nitroanilide + H2O -> Met_Leu_p_nitroanilide + Formate |
(( (( (kcat * E) ) * S) ) / (S + Km)) |
|
buffer: 50 MES, 50 mM bis-tris propane, 200 µM NiSO4, 1.0 unit Aeromonas proteolytica aminopeptidase
PH: 8
Temperature: 25 |
uniprot:P0A6K3 [peptide deformylase(Enzyme) wildtype]
N_Formyl_Met_Leu + H2O -> Formate + Met_Leu |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM potassium phosphate, 100 µM EGTA, 0.8 unit Vibrio proteolyticus aminopeptidase
PH: 7
Temperature: 23 |
uniprot:P0A6K3 [peptide deformylase(Enzyme) wildtype]
H2O + N_Formyl_Met_Leu_p_nitroanilide -> Met_Leu_p_nitroanilide + Formate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM potassium phosphate, 100 µM EGTA
PH: 7
Temperature: 23 |
uniprot:P0A6K3 [peptide deformylase(Enzyme) wildtype]
H2O + N_Formyl_Met_Leu_p_nitroanilide -> Formate + Met_Leu_p_nitroanilide |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM potassium phosphate, 100 µM EGTA, 0.8 unit Vibrio proteolyticus aminopeptidase
PH: 7
Temperature: 23 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) mutant deltaN45]
H2O + Formyl_L_methionyl_peptide -> Formate + Methionyl_peptide |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) mutant deltaN39]
Formyl_L_methionyl_peptide + H2O -> Formate + Methionyl_peptide |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) mutant deltaN19]
H2O + Formyl_L_methionyl_peptide -> Formate + Methionyl_peptide |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) mutant deltaN39]
Formyl_L_methionyl_peptide + H2O -> Methionyl_peptide + Formate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) mutant deltaN62 E91L]
Formyl_L_methionyl_peptide + H2O -> Methionyl_peptide + Formate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) mutant deltaN62]
H2O + Formyl_L_methionyl_peptide -> Methionyl_peptide + Formate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) mutant deltaN62 S47A]
Formyl_L_methionyl_peptide + H2O -> Methionyl_peptide + Formate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) mutant deltaN62]
H2O + Formyl_L_methionyl_peptide -> Methionyl_peptide + Formate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) mutant deltaN62 E91A]
H2O + Formyl_L_methionyl_peptide -> Methionyl_peptide + Formate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) mutant deltaN62]
H2O + Formyl_L_methionyl_peptide -> Formate + Methionyl_peptide |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) mutant deltaN62 W125L]
H2O + Formyl_L_methionyl_peptide -> Formate + Methionyl_peptide |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) mutant deltaN62 E91M]
H2O + Formyl_L_methionyl_peptide -> Formate + Methionyl_peptide |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) mutant deltaN62 C43G]
Formyl_L_methionyl_peptide + H2O -> Methionyl_peptide + Formate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) mutant deltaN62 C43G/E91L]
H2O + Formyl_L_methionyl_peptide -> Formate + Methionyl_peptide |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) mutant deltaN67]
H2O + Formyl_L_methionyl_peptide -> Formate + Methionyl_peptide |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) mutant deltaN62 S47A/E91L]
Formyl_L_methionyl_peptide + H2O -> Methionyl_peptide + Formate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) mutant deltaN67 G43C]
H2O + Formyl_L_methionyl_peptide -> Methionyl_peptide + Formate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) mutant deltaN62 C43G/S47A/E91L]
Formyl_L_methionyl_peptide + H2O -> Formate + Methionyl_peptide |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) mutant deltaN62 C43G/S47A/E91L]
Formyl_L_methionyl_peptide + H2O -> Methionyl_peptide + Formate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) mutant deltaN67 G43C]
H2O + Formyl_L_methionyl_peptide -> Methionyl_peptide + Formate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) mutant deltaN67 G43C/L91E]
Formyl_L_methionyl_peptide + H2O -> Methionyl_peptide + Formate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) mutant deltaN67 L91E]
Formyl_L_methionyl_peptide + H2O -> Methionyl_peptide + Formate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9FV54 [peptide deformylase(Enzyme) wildtype]
H2O + Formyl_L_methionyl_peptide -> Methionyl_peptide + Formate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) mutant deltaN39/R10Q/R11Q]
H2O + Formyl_L_methionyl_peptide -> Formate + Methionyl_peptide |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) wildtype]
Formyl_L_methionyl_peptide + H2O -> Methionyl_peptide + Formate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) mutant deltaN39/R13Q]
Formyl_L_methionyl_peptide + H2O -> Formate + Methionyl_peptide |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9FV53 [peptide deformylase(Enzyme) mutant deltaN67 W125L]
H2O + Formyl_L_methionyl_peptide -> Formate + Methionyl_peptide |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q9HBH1 [peptide deformylase(Enzyme) mutant deltaN45]
H2O + Formyl_L_methionyl_peptide -> Formate + Methionyl_peptide |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM KH2PO4, 12 mM NAD+, 1.2 U/ml Formate dehydrogenase
PH: 7.5
Temperature: 37 |