EC: 2.7.8.13

phospho-N-acetylmuramoyl-pentapeptide-transferase (UDP-MurAc(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala):undecaprenyl-phosphate phospho-N-acetylmuramoyl-pentapeptide-transferase)

enzyme lambda metabolic reaction experiment
uniprot:Q03521 [phospho-N-acetylmuramoyl-pentapeptide-transferase(Enzyme) wildtype MraY]

UDPMurAc(oyl_L_Ala_D_gamma_Glu_L_Lys_D_Ala_D_Ala) + Undecaprenyl_phosphate -> MurAc(oyl_L_Ala_D_gamma_Glu_L_Lys_D_Ala_D_Ala)_diphospho_undecaprenol + UMP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 40 mM MgCl2, 250 mM NaCl, 8.4 mM N-Lauroyl Sarcosine
PH: 7.5
Temperature: 37
uniprot:Q03521 [phospho-N-acetylmuramoyl-pentapeptide-transferase(Enzyme) wildtype MraY]

UDPMurAc(oyl_L_Ala_D_gamma_Glu_L_Lys_D_Ala_D_Ala) + Undecaprenyl_phosphate -> UMP + MurAc(oyl_L_Ala_D_gamma_Glu_L_Lys_D_Ala_D_Ala)_diphospho_undecaprenol		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 40 mM MgCl2, 250 mM NaCl, 8.4 mM N-Lauroyl Sarcosine
PH: 7.5
Temperature: 37
uniprot:Q5HGP9 [phospho-N-acetylmuramoyl-pentapeptide-transferase(Enzyme) wildtype]

Undecaprenyl_phosphate + UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanyl_D_alanine -> Undecaprenyl_diphospho_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanyl_D_alanine + UMP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Tris-HCl, 0.21 M KCl, 0.042 M MgCl2, 2.67 U/ml bacterial alkaline phosphatase
PH: 7.8
Temperature: 25
uniprot:Q5HGP9 [phospho-N-acetylmuramoyl-pentapeptide-transferase(Enzyme) wildtype]

UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanine + Undecaprenyl_phosphate -> Undecaprenyl_diphospho_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanine + UMP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Tris-HCl, 0.21 M KCl, 0.042 M MgCl2, 2.67 U/ml bacterial alkaline phosphatase
PH: 7.8
Temperature: 25
uniprot:Q5HGP9 [phospho-N-acetylmuramoyl-pentapeptide-transferase(Enzyme) wildtype]

Undecaprenyl_phosphate + UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysine -> UMP + Undecaprenyl_diphospho_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Tris-HCl, 0.21 M KCl, 0.042 M MgCl2, 2.67 U/ml bacterial alkaline phosphatase
PH: 7.8
Temperature: 25
uniprot:Q5HGP9 [phospho-N-acetylmuramoyl-pentapeptide-transferase(Enzyme) wildtype]

UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_glycyl_D_alanine + Undecaprenyl_phosphate -> Undecaprenyl_diphospho_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_glycyl_D_alanine + UMP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Tris-HCl, 0.21 M KCl, 0.042 M MgCl2, 2.67 U/ml bacterial alkaline phosphatase
PH: 7.8
Temperature: 25
uniprot:Q5HGP9 [phospho-N-acetylmuramoyl-pentapeptide-transferase(Enzyme) wildtype]

Undecaprenyl_phosphate + UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanyl_glycine -> UMP + Undecaprenyl_diphospho_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanyl_glycine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Tris-HCl, 0.21 M KCl, 0.042 M MgCl2, 2.67 U/ml bacterial alkaline phosphatase
PH: 7.8
Temperature: 25
uniprot:Q5HGP9 [phospho-N-acetylmuramoyl-pentapeptide-transferase(Enzyme) wildtype]

UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_diaminopimelic_acid_D_alanyl_D_alanine + Undecaprenyl_phosphate -> Undecaprenyl_diphospho_N_acetylmuramoyl_L_alanyl_D_glutamyl_diaminopimelic_acid_D_alanyl_D_alanine + UMP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Tris-HCl, 0.21 M KCl, 0.042 M MgCl2, 2.67 U/ml bacterial alkaline phosphatase
PH: 7.8
Temperature: 25
uniprot:Q5HGP9 [phospho-N-acetylmuramoyl-pentapeptide-transferase(Enzyme) wildtype]

Undecaprenyl_phosphate + UDP_N_acetylmuramoyl_glycyl_D_glutamyl_L_lysyl_L_alanyl_D_alanine -> UMP + Undecaprenyl_diphospho_N_acetylmuramoyl_glycyl_D_glutamyl_L_lysyl_L_alanyl_D_alanine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Tris-HCl, 0.21 M KCl, 0.042 M MgCl2, 2.67 U/ml bacterial alkaline phosphatase
PH: 7.8
Temperature: 25

Pathways

pathway id name
BioCyc:ECO_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:GCF_000013425_PWY-6470 peptidoglycan biosynthesis V (β-lactam resistance)
BioCyc:META_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:META_PWY-5265 peptidoglycan biosynthesis II (staphylococci)
BioCyc:VCHO_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:ANTHRA_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:META_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:AGRO_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:SYNEL_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:ECOL316407_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:PDIF272563_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:BTHE_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:BTHE_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:CORYNE_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:MTBH37RV_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:HPY_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:ECOL199310_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:ECOL199310_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:GCF_000013425_PWY-5265 peptidoglycan biosynthesis II (staphylococci)
PathBank:SMP0000924 Peptidoglycan Biosynthesis I
PathBank:SMP0002074 Peptidoglycan Biosynthesis II
PathBank:SMP0122239 Peptidoglycan Biosynthesis II
PathBank:SMP0121305 Peptidoglycan Biosynthesis I