EC: 2.7.3.9

phosphoenolpyruvate-protein phosphotransferase (phosphoenolpyruvate:protein-L-histidine Npi-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:P0A249 [Mg2+]

Protein_N_phosphohistidine + Pyruvate -> Phosphoenolpyruvate + Protein_histidine
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 10 mM KF
PH: 7.5
Temperature: 37
uniprot:P0A249 [Mg2+]

Phosphoenolpyruvate + Protein_histidine -> Protein_N_phosphohistidine + Pyruvate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 10 mM KF
PH: 7.5
Temperature: 37
uniprot:P08839 [phosphoenolpyruvate---protein phosphotransferase(Enzyme) mutant C502A]

Phosphoenolpyruvate + Protein_histidine -> Protein_N_phosphohistidine + Pyruvate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [phosphoenolpyruvate---protein phosphotransferase(Enzyme) wildtype]

Protein_histidine + Phosphoenolpyruvate -> Pyruvate + Protein_N_phosphohistidine
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [phosphoenolpyruvate---protein phosphotransferase(Enzyme) mutant C502A]

Protein_histidine + (Z)_3_Fluoro_phosphoenolpyruvate -> 3_Fluoropyruvate + Protein_N_phosphohistidine
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [phosphoenolpyruvate---protein phosphotransferase(Enzyme) wildtype]

(Z)_3_Fluoro_phosphoenolpyruvate + Protein_histidine -> Protein_N_phosphohistidine + 3_Fluoropyruvate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [phosphoenolpyruvate---protein phosphotransferase(Enzyme) wildtype]

Protein_histidine + (Z)_3_Methyl_phosphoenolpyruvate -> 2_Oxobutyrate + Protein_N_phosphohistidine
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [phosphoenolpyruvate---protein phosphotransferase(Enzyme) wildtype]

Protein_histidine + (E)_3_Chloro_phosphoenolpyruvate -> Protein_N_phosphohistidine + 3_Chloropyruvate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [phosphoenolpyruvate---protein phosphotransferase(Enzyme) wildtype]

(Z)_3_Chloro_phosphoenolpyruvate + Protein_histidine -> 3_Chloropyruvate + Protein_N_phosphohistidine
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [phosphoenolpyruvate---protein phosphotransferase(Enzyme) mutant C502A]

(Z)_3_Chloro_phosphoenolpyruvate + Protein_histidine -> 3_Chloropyruvate + Protein_N_phosphohistidine
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [phosphoenolpyruvate---protein phosphotransferase(Enzyme) wildtype]

Protein_histidine + Phosphoenolpyruvate -> Protein_N_phosphohistidine + Pyruvate
(( (( (E * kcat) ) * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [Oxalate]

Protein_histidine + Phosphoenolpyruvate -> Protein_N_phosphohistidine + Pyruvate
(( (( (E * kcat) ) * A) ) / (( (Ks * (1 + (I / Ki1))) ) + (( (1 + (I / Ki2)) ) * A))) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [phosphoenolpyruvate---protein phosphotransferase(Enzyme) wildtype]

Protein_histidine + Phosphoenolpyruvate -> Protein_N_phosphohistidine + Pyruvate
(( (( (E * kcat) ) * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [Oxalate]

Protein_histidine + (Z)_3_Methyl_phosphoenolpyruvate -> 2_Oxobutyrate + Protein_N_phosphohistidine
(( (( (E * kcat) ) * A) ) / (( (Ks * (1 + (I / Ki1))) ) + (( (1 + (I / Ki2)) ) * A))) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [Oxalate]

(Z)_3_Fluoro_phosphoenolpyruvate + Protein_histidine -> Protein_N_phosphohistidine + 3_Fluoropyruvate
(( (( (E * kcat) ) * A) ) / (( (Ks * (1 + (I / Ki1))) ) + (( (1 + (I / Ki2)) ) * A))) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [Oxalate]

Protein_histidine + (Z)_3_Fluoro_phosphoenolpyruvate -> Protein_N_phosphohistidine + 3_Fluoropyruvate
(( (( (E * kcat) ) * A) ) / (( (Ks * (1 + (I / Ki1))) ) + (( (1 + (I / Ki2)) ) * A))) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [Oxalate]

(Z)_3_Chloro_phosphoenolpyruvate + Protein_histidine -> 3_Chloropyruvate + Protein_N_phosphohistidine
(( (( (E * kcat) ) * A) ) / (( (Ks * (1 + (I / Ki1))) ) + (( (1 + (I / Ki2)) ) * A))) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [Oxalate]

(Z)_3_Chloro_phosphoenolpyruvate + Protein_histidine -> Protein_N_phosphohistidine + 3_Chloropyruvate
(( (( (E * kcat) ) * A) ) / (( (Ks * (1 + (I / Ki1))) ) + (( (1 + (I / Ki2)) ) * A))) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [phosphoenolpyruvate---protein phosphotransferase(Enzyme) mutant C502A]

Protein_histidine + (E)_3_Chloro_phosphoenolpyruvate -> Protein_N_phosphohistidine + 3_Chloropyruvate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [(E)-3-Chloro-phosphoenolpyruvate]

Protein_histidine + Phosphoenolpyruvate -> Protein_N_phosphohistidine + Pyruvate
(( (( (E * kcat) ) * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [phosphoenolpyruvate---protein phosphotransferase(Enzyme) wildtype]

Protein_histidine + Phosphoenolpyruvate -> Pyruvate + Protein_N_phosphohistidine
(( (( (E * kcat) ) * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [Phosphoenolpyruvate]

(Z)_3_Chloro_phosphoenolpyruvate + Protein_histidine -> 3_Chloropyruvate + Protein_N_phosphohistidine
(( (( (E * kcat) ) * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [phosphoenolpyruvate---protein phosphotransferase(Enzyme) mutant C502A]

(Z)_3_Chloro_phosphoenolpyruvate + Protein_histidine -> Protein_N_phosphohistidine + 3_Chloropyruvate
(( (( (E * kcat) ) * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [phosphoenolpyruvate---protein phosphotransferase(Enzyme) wildtype]

Protein_histidine + Phosphoenolpyruvate -> Protein_N_phosphohistidine + Pyruvate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [phosphoenolpyruvate---protein phosphotransferase(Enzyme) mutant C502A]

(Z)_3_Chloro_phosphoenolpyruvate + Protein_histidine -> Protein_N_phosphohistidine + 3_Chloropyruvate
(( (( (E * kcat) ) * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [(E)-3-Chloro-phosphoenolpyruvate]

Protein_histidine + (Z)_3_Chloro_phosphoenolpyruvate -> Protein_N_phosphohistidine + 3_Chloropyruvate
(( (( (E * kcat) ) * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P08839 [phosphoenolpyruvate---protein phosphotransferase(Enzyme) wildtype]

(Z)_3_Chloro_phosphoenolpyruvate + Protein_histidine -> Protein_N_phosphohistidine + 3_Chloropyruvate
(( (( (( (E * exp((( (0 - k_i) ) * t))) ) * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 10 mM KCN, 1 mg/ml BSA
PH: 7.5
Temperature: 30

Pathways

pathway id name
WikiPathways:WP2472 Escherichia coli K-12 peripherome
PathBank:SMP0000930 Fructose Metabolism