EC: 2.7.2.2

carbamate kinase (ATP:carbamate phosphotransferase)

enzyme lambda metabolic reaction experiment
- [carbamate kinase(Enzyme) wildtype]

ATP + Carbamate -> Carbamoyl_phosphate + ADP		 (( (Vmax * B) ) / (Km + B)) buffer: 286 mM Tris, 28,6 mM MgCl2, 14.3 mM L-ornithine, 57.1 U/ml ornithine transcarbamylase (purified from Enterococcus faecalis)
PH: 8.5
Temperature: 37
- [carbamate kinase(Enzyme) wildtype]

ATP + Carbamate -> ADP + Carbamoyl_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 286 mM Tris, 28,6 mM MgCl2, 14.3 mM L-ornithine, 57.1 U/ml ornithine transcarbamylase (purified from Enterococcus faecalis)
PH: 8.5
Temperature: 37
uniprot:P37306 [carbamate kinase(Enzyme) wildtype]

ATP + Carbamate -> Carbamoyl_phosphate + ADP		 (( (Vmax * B) ) / (Km + B)) buffer: 286 mM Tris, 28,6 mM MgCl2, 14.3 mM L-ornithine, 57.1 U/ml ornithine transcarbamylase (purified from Enterococcus faecalis)
PH: 8.5
Temperature: 37
uniprot:P37306 [carbamate kinase(Enzyme) wildtype]

Carbamate + ATP -> ADP + Carbamoyl_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 286 mM Tris, 28,6 mM MgCl2, 14.3 mM L-ornithine, 57.1 U/ml ornithine transcarbamylase (purified from Enterococcus faecalis)
PH: 8.5
Temperature: 37
- [carbamate kinase(Enzyme) wildtype]

ATP + Acetate -> Acetyl_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 35.7 mM Tris, 7.1 mM MgCl2, 0.08 mM CoA, 19.3 U/ml transacetylase
PH: 7.4
Temperature: 29
- [carbamate kinase(Enzyme) wildtype]

ATP + Acetate -> Acetyl_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 35.7 mM Tris, 7.1 mM MgCl2, 0.08 mM CoA, 19.3 U/ml transacetylase
PH: 7.4
Temperature: 29
- [carbamate kinase(Enzyme) wildtype]

ADP + Carbamoyl_phosphate -> ATP + NH3 + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: 1 M Acetate, 5 mM Mg2+
PH: 5
Temperature: 30
- [carbamate kinase(Enzyme) wildtype]

CO2 + NH3 + ATP -> Carbamoyl_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Tris, 10 mM L-ornithine, 100 units/ml ornithine transcarbamylase, 5 mM Mg2+
PH: 8.5
Temperature: 37
- [carbamate kinase(Enzyme) wildtype]

Carbamate + ATP -> Carbamoyl_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 500 mM acetate, 80 mM Mg2+
PH: 8.4
Temperature: 30

Pathways

pathway id name
BioCyc:ECO_PWY0-41 allantoin degradation IV (anaerobic)
BioCyc:ECOO157_CITRULLINE-DEG-PWY citrulline degradation
BioCyc:ECOL199310_CITRULLINE-DEG-PWY citrulline degradation
BioCyc:ECOL199310_ARGDEGRAD-PWY arginine degradation V (arginine deiminase pathway)
BioCyc:META_CITRULLINE-DEG-PWY L-citrulline degradation
BioCyc:META_ARGDEGRAD-PWY L-arginine degradation V (arginine deiminase pathway)
BioCyc:ECOL316407_PWY0-41 allantoin degradation IV (anaerobic)
BioCyc:GCF_000013425_ARGDEGRAD-PWY L-arginine degradation V (arginine deiminase pathway)
BioCyc:ECOL199310_PWY0-41 allantoin degradation IV (anaerobic)
BioCyc:GCF_000013425_CITRULLINE-DEG-PWY L-citrulline degradation
PathBank:SMP0000778 Nitrogen Metabolism
PathBank:SMP0002064 Allantoin Degradation (Anaerobic)