EC: 2.7.1.4

fructokinase (ATP:D-fructose 6-phosphotransferase)

enzyme lambda metabolic reaction experiment
- [D-Glucose]

ATP + D_Fructose -> D_Fructose_6_phosphate + ADP		 (( (( (( (( (( (vol * Vmax4) ) * Fru) ) / Km4Fru) ) * ATP) ) / Km4ATP) ) / (( (1 + (ATP / Km4ATP)) ) * (( (( (( (1 + (Glc / Km3Glc)) ) + (Fru / Km4Fru)) ) + (( (0.113 * HexP) ) / Ki3G6P)) ) + (( (0.0575 * HexP) ) / Ki4F6P)))) buffer: *
PH: 7
Temperature: 36
- [fructokinase(Enzyme) wildtype]

ATP + D_Fructose -> ADP + D_Fructose_6_phosphate		 (( (( (( (( (( (( (vol * Vmax5) ) / (1 + (Fru / Ki5Fru))) ) * Fru) ) / Km5Fru) ) * ATP) ) / Km5ATP) ) / (( (( (( (1 + (Fru / Km5Fru)) ) + (ATP / Km5ATP)) ) + (( (Fru * ATP) ) / (Km5Fru * Km5ATP))) ) + (ADP / Ki5ADP))) buffer: *
PH: 7
Temperature: 36
- [fructokinase(Enzyme) wildtype]

D_Fructose + ATP -> ADP + D_Fructose_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Bicine-NaOH, 5 mM MgCl2, 1 mM NAD, 1 IU/ml glucose 6-P dehydrogenase, 1 IU/ml phosphoglucoisomerase
PH: 8.5
Temperature: 30
- [fructokinase(Enzyme) wildtype]

D_Fructose + ATP -> D_Fructose_6_phosphate + ADP		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Bicine-NaOH, 5 mM MgCl2, 1 mM NAD, 1 IU/ml glucose 6-P dehydrogenase, 1 IU/ml phosphoglucoisomerase
PH: 8.5
Temperature: 30
- [fructokinase(Enzyme) wildtype]

D_Fructose + ATP -> D_Fructose_6_phosphate + ADP		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Bicine-NaOH, 5 mM MgCl2, 1 mM NAD, 1 IU/ml glucose 6-P dehydrogenase, 1 IU/ml phosphoglucoisomerase
PH: 8.5
Temperature: 30
- [fructokinase(Enzyme) wildtype]

ATP + D_Fructose -> ADP + D_Fructose_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Bicine-NaOH, 5 mM MgCl2, 1 mM NAD, 1 IU/ml glucose 6-P dehydrogenase, 1 IU/ml phosphoglucoisomerase
PH: 8.5
Temperature: 30
- [Mg2+]

Fructose + ATP -> ADP + Fructose_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 1 mM NADP+, 25 U/ml glucose 6-phosphate dehydrogenase, 25 U/ml phosphoglucose isomerase
PH: 7
Temperature: 22
- [Mg2+]

Fructose + ATP -> ADP + Fructose_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 1 mM NADP+, 25 U/ml glucose 6-phosphate dehydrogenase, 25 U/ml phosphoglucose isomerase
PH: 7
Temperature: 22
- [Mg2+]

ATP + Fructose -> ADP + Fructose_6_phosphate		 (( (Vmax * M) ) / (Km + M)) buffer: 0.1 M potassium phosphate, 1 mM NADP+, 25 U/ml glucose 6-phosphate dehydrogenase, 25 U/ml phosphoglucose isomerase
PH: 7
Temperature: 22
- [beta,gamma-Methylene ATP]

ATP + Fructose -> ADP + Fructose_6_phosphate		 (( (V * A) ) / (A + (Ka * (1 + (I / Kis))))) buffer: 25 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl2, 0.33 mM NADP, 100 µg/ml BSA, 3 µg/ml phosphoglucose isomerase, 0.6 µg/ml glucose-6-P dehydrogenase
PH: 8
Temperature: 30
- [Mg2+]

ATP + Fructose -> ADP + Fructose_6_phosphate		 (( (( (( (V * A) ) * B) ) * (1 + (B / KI3))) ) / (( (( (( (Kia * Kb) ) + (( (Ka * B) ) * (1 + (B / KI1)))) ) + (Kb * A)) ) + (( (A * B) ) * (1 + (B / KI2))))) buffer: 25 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl2, 0.33 mM NADP, 100 µg/ml BSA, 3 µg/ml phosphoglucose isomerase, 0.6 µg/ml glucose-6-P dehydrogenase
PH: 8
Temperature: 30
- [Mg2+]

ATP + Fructose -> ADP + Fructose_6_phosphate		 (( (( (V * A) ) * B) ) / (( (( (( (Kia * Kb) ) + (Ka * B)) ) + (Kb * A)) ) + (( (A * B) ) * (1 + (A / KIa))))) buffer: 25 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl2, 0.33 mM NADP, 100 µg/ml BSA, 3 µg/ml phosphoglucose isomerase, 0.6 µg/ml glucose-6-P dehydrogenase
PH: 8
Temperature: 30
- [Mg2+]

ATP + Fructose -> ADP + Fructose_6_phosphate		 (( (V * A) ) / (( (A * (1 + (I / Kim))) ) + Ka)) buffer: 25 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl2, 0.33 mM NADP, 100 µg/ml BSA, 3 µg/ml phosphoglucose isomerase, 0.6 µg/ml glucose-6-P dehydrogenase
PH: 8
Temperature: 30
- [beta,gamma-Methylene ATP]

ATP + Fructose -> ADP + Fructose_6_phosphate		 (( (V * A) ) / (( (A * (1 + (I / Kii))) ) + (Ka * (1 + (I / Kis))))) buffer: 25 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl2, 0.33 mM NADP, 100 µg/ml BSA, 3 µg/ml phosphoglucose isomerase, 0.6 µg/ml glucose-6-P dehydrogenase
PH: 8
Temperature: 30
- [L-Sorbose]

Fructose + ATP -> ADP + Fructose_6_phosphate		 (( (V * A) ) / (A + (Ka * (1 + (I / Kis))))) buffer: 25 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl2, 0.33 mM NADP, 100 µg/ml BSA, 3 µg/ml phosphoglucose isomerase, 0.6 µg/ml glucose-6-P dehydrogenase
PH: 8
Temperature: 30
- [L-Sorbose]

ATP + Fructose -> ADP + Fructose_6_phosphate		 (( (V * A) ) / (( (A * (1 + (I / Kii))) ) + Ka)) buffer: 25 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl2, 0.33 mM NADP, 100 µg/ml BSA, 3 µg/ml phosphoglucose isomerase, 0.6 µg/ml glucose-6-P dehydrogenase
PH: 8
Temperature: 30
- [Mg2+]

Fructose + ATP -> ADP + Fructose_6_phosphate		 (( (( (V * A) ) * (1 + (I / Kih))) ) / (( (A * (1 + (I / Kii))) ) + (Ka * (1 + (I / Kis))))) buffer: 25 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl2, 0.33 mM NADP, 100 µg/ml BSA, 3 µg/ml phosphoglucose isomerase, 0.6 µg/ml glucose-6-P dehydrogenase
PH: 8
Temperature: 30
- [Mg2+]

ATP + Fructose -> Fructose_6_phosphate + ADP		 (( (( (V * A) ) * (1 + (I / Kih))) ) / (( (A * (1 + (I / Kii))) ) + (Ka * (1 + (I / Kis))))) buffer: 25 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl2, 0.33 mM NADP, 100 µg/ml BSA, 3 µg/ml phosphoglucose isomerase, 0.6 µg/ml glucose-6-P dehydrogenase
PH: 8
Temperature: 30
- [Mg2+]

Fructose + ATP -> ADP + Fructose_6_phosphate		 (( (( (V * A) ) * (1 + (I / Kih))) ) / (( (A * (1 + (I / Kii))) ) + (Ka * (1 + (I / Kis))))) buffer: 25 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl2, 0.33 mM NADP, 100 µg/ml BSA, 3 µg/ml phosphoglucose isomerase, 0.6 µg/ml glucose-6-P dehydrogenase
PH: 8
Temperature: 30
- [Mg2+]

ATP + Fructose -> ADP + Fructose_6_phosphate		 (( (V * A) ) / (( (A * (1 + (I / Kim))) ) + Ka)) buffer: 25 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl2, 0.33 mM NADP, 100 µg/ml BSA, 3 µg/ml phosphoglucose isomerase, 0.6 µg/ml glucose-6-P dehydrogenase
PH: 8
Temperature: 30
- [Mg2+]

Fructose + ATP -> ADP + Fructose_6_phosphate		 (( (V * A) ) / (( (A * (1 + (I / Kii))) ) + (Ka * (1 + (I / Kis))))) buffer: 25 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl2, 0.33 mM NADP, 100 µg/ml BSA, 3 µg/ml phosphoglucose isomerase, 0.6 µg/ml glucose-6-P dehydrogenase
PH: 8
Temperature: 30
- [Mg2+]

Fructose + ATP -> Fructose_6_phosphate + ADP		 (( (V * A) ) / (( (A * (1 + (I / Kii))) ) + (Ka * (1 + (I / Kis))))) buffer: 25 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl2, 0.33 mM NADP, 100 µg/ml BSA, 3 µg/ml phosphoglucose isomerase, 0.6 µg/ml glucose-6-P dehydrogenase
PH: 8
Temperature: 30
uniprot:A2WXV8 [fructokinase(Enzyme) wildtype]

ATP + D_Fructose -> D_Fructose_6_phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 30 mM HEPES-KOH, 1 mM MgCl2, 0.6 mM EDTA, 9 mM KCl, 1 mM NADP+, 2 U/ml NADP+ dependent Glucose 6-phosphate dehydrogenase, 2 U/ml phosphoglucoisomerase
PH: 7.5
Temperature: 37
uniprot:Q09124 [Mg2+]

ATP + D_Fructose -> D_Fructose_6_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M imidazole-HCl, 1 mM NADP+, 25 units glucose 6-phosphate dehydrogenase, 25 units phosphoglucose isomerase
PH: 7.5
Temperature: 36
uniprot:Q09124 [Mg2+]

D_Fructose + ATP -> D_Fructose_6_phosphate + ADP		 (( (Vmax * C) ) / (Km + C)) buffer: 0.1 M imidazole-HCl, 1 mM NADP+, 25 units glucose 6-phosphate dehydrogenase, 25 units phosphoglucose isomerase
PH: 7.5
Temperature: 36
uniprot:Q09124 [Mg2+]

D_Fructose + ATP -> D_Fructose_6_phosphate + ADP		 (( (Vmax * B) ) / (Km + B)) buffer: 0.1 M imidazole-HCl, 1 mM NADP+, 25 units glucose 6-phosphate dehydrogenase, 25 units phosphoglucose isomerase
PH: 7.5
Temperature: 36
uniprot:P23917 [fructokinase(Enzyme) wildtype]

D_Glucose + ATP -> D_Glucose_6_phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.3-0.5 M Tris-HCl
PH: 7.6
Temperature: 25
uniprot:P23917 [fructokinase(Enzyme) wildtype]

D_Glucose + ATP -> D_Glucose_6_phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.3-0.5 M Tris-HCl
PH: 7.6
Temperature: 25
uniprot:P23917 [fructokinase(Enzyme) wildtype]

D_Fructose + ATP -> D_Fructose_6_phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.3-0.5 M Tris-HCl
PH: 7.6
Temperature: 25
uniprot:P23917 [fructokinase(Enzyme) wildtype]

D_Fructose + ATP -> D_Fructose_6_phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.3-0.5 M Tris-HCl
PH: 7.6
Temperature: 25
uniprot:P37829 [fructokinase(Enzyme) wildtype isoenzyme FK-I]

ATP + Fructose -> Fructose_6_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 5 mM MgSO4, 0.2 mM NAD, 2 U/ml phosphoglucose isomerase, 1.5 U/ml glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides
PH: 8.5
Temperature: 25
uniprot:P37829 [fructokinase(Enzyme) wildtype isoenzyme FK-III]

Fructose + ATP -> Fructose_6_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 5 mM MgSO4, 0.2 mM NAD, 2 U/ml phosphoglucose isomerase, 1.5 U/ml glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides
PH: 8.5
Temperature: 25
uniprot:P37829 [fructokinase(Enzyme) wildtype isoenzyme FK-II]

ATP + Fructose -> ADP + Fructose_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 5 mM MgSO4, 0.2 mM NAD, 2 U/ml phosphoglucose isomerase, 1.5 U/ml glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides
PH: 8.5
Temperature: 25
uniprot:P37829 [fructokinase(Enzyme) wildtype isoenzyme FK-III]

Fructose + ATP -> Fructose_6_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 5 mM MgSO4, 0.2 mM NAD, 2 U/ml phosphoglucose isomerase, 1.5 U/ml glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides
PH: 8.5
Temperature: 25
uniprot:P37829 [fructokinase(Enzyme) wildtype isoenzyme FK-I]

ATP + Fructose -> Fructose_6_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 5 mM MgSO4, 0.2 mM NAD, 2 U/ml phosphoglucose isomerase, 1.5 U/ml glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides
PH: 8.5
Temperature: 25
uniprot:P37829 [fructokinase(Enzyme) wildtype isoenzyme FK-II]

ATP + Fructose -> ADP + Fructose_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 5 mM MgSO4, 0.2 mM NAD, 2 U/ml phosphoglucose isomerase, 1.5 U/ml glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides
PH: 8.5
Temperature: 25
uniprot:P37829 [fructokinase(Enzyme) wildtype isoenzyme FK-I]

UTP + Fructose -> UDP + Fructose_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 5 mM MgSO4, 0.2 mM NAD, 2 U/ml phosphoglucose isomerase, 1.5 U/ml glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides
PH: 8.5
Temperature: 25
uniprot:P37829 [fructokinase(Enzyme) wildtype isoenzyme FK-I]

Fructose + GTP -> GDP + Fructose_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 5 mM MgSO4, 0.2 mM NAD, 2 U/ml phosphoglucose isomerase, 1.5 U/ml glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides
PH: 8.5
Temperature: 25
uniprot:P37829 [fructokinase(Enzyme) wildtype isoenzyme FK-II]

GTP + Fructose -> GDP + Fructose_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 5 mM MgSO4, 0.2 mM NAD, 2 U/ml phosphoglucose isomerase, 1.5 U/ml glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides
PH: 8.5
Temperature: 25
uniprot:P37829 [fructokinase(Enzyme) wildtype isoenzyme FK-III]

GTP + Fructose -> Fructose_6_phosphate + GDP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 5 mM MgSO4, 0.2 mM NAD, 2 U/ml phosphoglucose isomerase, 1.5 U/ml glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides
PH: 8.5
Temperature: 25
uniprot:P37829 [fructokinase(Enzyme) wildtype isoenzyme FK-III]

Fructose + UTP -> UDP + Fructose_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 5 mM MgSO4, 0.2 mM NAD, 2 U/ml phosphoglucose isomerase, 1.5 U/ml glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides
PH: 8.5
Temperature: 25
uniprot:P37829 [fructokinase(Enzyme) wildtype isoenzyme FK-II]

UTP + Fructose -> Fructose_6_phosphate + UDP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 5 mM MgSO4, 0.2 mM NAD, 2 U/ml phosphoglucose isomerase, 1.5 U/ml glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides
PH: 8.5
Temperature: 25
uniprot:P37829 [fructokinase(Enzyme) wildtype isoenzyme FK-II]

Fructose + CTP -> Fructose_6_phosphate + CDP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 5 mM MgSO4, 0.2 mM NAD, 2 U/ml phosphoglucose isomerase, 1.5 U/ml glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides
PH: 8.5
Temperature: 25
uniprot:P37829 [fructokinase(Enzyme) wildtype isoenzyme FK-III]

Fructose + CTP -> CDP + Fructose_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 5 mM MgSO4, 0.2 mM NAD, 2 U/ml phosphoglucose isomerase, 1.5 U/ml glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides
PH: 8.5
Temperature: 25
uniprot:P37829 [fructokinase(Enzyme) wildtype isoenzyme FK-I]

Fructose + CTP -> CDP + Fructose_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 5 mM MgSO4, 0.2 mM NAD, 2 U/ml phosphoglucose isomerase, 1.5 U/ml glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides
PH: 8.5
Temperature: 25

Pathways

pathway id name
BioCyc:ARA_PWYQT-4466 superpathway of sucrose and starch metabolism I (non-photosynthetic tissue)
BioCyc:BSUB_SUCUTIL-PWY sucrose degradation I (sucrose phosphotransferase)
BioCyc:BSUB_PWY-4101 D-sorbitol degradation I
BioCyc:ARA_PWY-3801 sucrose degradation II (sucrose synthase)
BioCyc:ARA_PWY-4101 D-sorbitol degradation I
BioCyc:ARA_PWY-621 sucrose degradation III (sucrose invertase)
BioCyc:META_SUCUTIL-PWY sucrose degradation I (sucrose phosphotransferase)
PathBank:SMP0000958 Starch and Sucrose Metabolism
PlantCyc:ARA_PWY-621 sucrose degradation III (sucrose invertase)
PlantCyc:ARA_PWY-4101 D-sorbitol degradation I
PlantCyc:ARA_PWY-7345 superpathway of anaerobic sucrose degradation
PlantCyc:ARA_PWY-3801 sucrose degradation II (sucrose synthase)
PlantCyc:ARA_PWYQT-4466 superpathway of sucrose and starch metabolism I (non-photosynthetic tissue)
PathBank:SMP0012445 Fructose Metabolism