EC: 2.4.2.3

uridine phosphorylase (uridine:phosphate alpha-D-ribosyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:O95045 [uridine phosphorylase(Enzyme) wildtype isoenzym Upp2]

Phosphate + 2'_Deoxyuridine -> 2_Deoxy_D_ribose_1_phosphate + Uracil		 (( (V * S) ) / (Km + S)) buffer: 20 mM potassium phosphate, 1 mM dithiothreitol, 1 mM EDTA
PH: 8
Temperature: 37
uniprot:O95045 [uridine phosphorylase(Enzyme) wildtype isoenzym Upp2]

Thymidine + Phosphate -> Thymine + 2_Deoxy_D_ribose_1_phosphate		 (( (V * S) ) / (Km + S)) buffer: 20 mM potassium phosphate, 1 mM dithiothreitol, 1 mM EDTA
PH: 8
Temperature: 37
uniprot:O95045 [uridine phosphorylase(Enzyme) wildtype isoenzym Upp2]

5_Fluorodeoxyuridine + Phosphate -> 5_FU + 2_Deoxy_D_ribose_1_phosphate		 (( (V * S) ) / (Km + S)) buffer: 20 mM potassium phosphate, 1 mM dithiothreitol, 1 mM EDTA
PH: 8
Temperature: 37
uniprot:O95045 [uridine phosphorylase(Enzyme) wildtype isoenzym Upp2]

Phosphate + 5_Fluorouridine -> D_Ribose_1_phosphate + 5_FU		 (( (V * S) ) / (Km + S)) buffer: 20 mM potassium phosphate, 1 mM dithiothreitol, 1 mM EDTA
PH: 8
Temperature: 37
uniprot:O95045 [uridine phosphorylase(Enzyme) wildtype isoenzym Upp2]

Uridine + Phosphate -> D_Ribose_1_phosphate + Uracil		 (( (V * S) ) / (Km + S)) buffer: 20 mM potassium phosphate, 1 mM dithiothreitol, 1 mM EDTA
PH: 8
Temperature: 37
uniprot:O95045 [uridine phosphorylase(Enzyme) wildtype isoenzym Upp2]

Doxifluridine + Phosphate -> 5_Deoxy_D_ribose_1_phosphate + 5_FU		 (( (V * S) ) / (Km + S)) buffer: 20 mM potassium phosphate, 1 mM dithiothreitol, 1 mM EDTA
PH: 8
Temperature: 37
uniprot:Q8CGR7 [uridine phosphorylase(Enzyme) wildtype isoenzym Upp2]

Phosphate + 5_Fluorodeoxyuridine -> 5_FU + 2_Deoxy_D_ribose_1_phosphate		 (( (V * S) ) / (Km + S)) buffer: 20 mM potassium phosphate, 1 mM dithiothreitol, 1 mM EDTA
PH: 8
Temperature: 37
uniprot:Q8CGR7 [uridine phosphorylase(Enzyme) wildtype isoenzym Upp2]

5_Fluorouridine + Phosphate -> D_Ribose_1_phosphate + 5_FU		 (( (V * S) ) / (Km + S)) buffer: 20 mM potassium phosphate, 1 mM dithiothreitol, 1 mM EDTA
PH: 8
Temperature: 37
uniprot:Q8CGR7 [uridine phosphorylase(Enzyme) wildtype isoenzym Upp2]

Phosphate + Doxifluridine -> 5_FU + 5_Deoxy_D_ribose_1_phosphate		 (( (V * S) ) / (Km + S)) buffer: 20 mM potassium phosphate, 1 mM dithiothreitol, 1 mM EDTA
PH: 8
Temperature: 37
uniprot:Q8CGR7 [uridine phosphorylase(Enzyme) wildtype isoenzym Upp2]

Uridine + Phosphate -> Uracil + alpha_D_Ribose_1_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Tris-HCl
PH: 7.4
Temperature: 37
uniprot:Q16831 [uridine phosphorylase(Enzyme) wildtype isoenzym Upp1]

2'_Deoxyuridine + Phosphate -> 2_Deoxy_D_ribose_1_phosphate + Uracil		 (( (V * S) ) / (Km + S)) buffer: 20 mM potassium phosphate, 1 mM dithiothreitol, 1 mM EDTA
PH: 8
Temperature: 37
uniprot:Q16831 [uridine phosphorylase(Enzyme) wildtype isoenzym Upp1]

Thymidine + Phosphate -> Thymine + 2_Deoxy_D_ribose_1_phosphate		 (( (V * S) ) / (Km + S)) buffer: 20 mM potassium phosphate, 1 mM dithiothreitol, 1 mM EDTA
PH: 8
Temperature: 37
uniprot:Q16831 [uridine phosphorylase(Enzyme) wildtype isoenzym Upp1]

5_Fluorodeoxyuridine + Phosphate -> 5_FU + 2_Deoxy_D_ribose_1_phosphate		 (( (V * S) ) / (Km + S)) buffer: 20 mM potassium phosphate, 1 mM dithiothreitol, 1 mM EDTA
PH: 8
Temperature: 37
uniprot:Q8CGR7 [uridine phosphorylase(Enzyme) wildtype isoenzym Upp2]

Phosphate + 2'_Deoxyuridine -> Uracil + 2_Deoxy_D_ribose_1_phosphate		 (( (V * S) ) / (Km + S)) buffer: 20 mM potassium phosphate, 1 mM dithiothreitol, 1 mM EDTA
PH: 8
Temperature: 37
uniprot:Q8CGR7 [uridine phosphorylase(Enzyme) wildtype isoenzym Upp2]

Thymidine + Phosphate -> 2_Deoxy_D_ribose_1_phosphate + Thymine		 (( (V * S) ) / (Km + S)) buffer: 20 mM potassium phosphate, 1 mM dithiothreitol, 1 mM EDTA
PH: 8
Temperature: 37
uniprot:Q16831 [uridine phosphorylase(Enzyme) wildtype isoenzym Upp1]

5_Fluorouridine + Phosphate -> D_Ribose_1_phosphate + 5_FU		 (( (V * S) ) / (Km + S)) buffer: 20 mM potassium phosphate, 1 mM dithiothreitol, 1 mM EDTA
PH: 8
Temperature: 37
uniprot:Q16831 [uridine phosphorylase(Enzyme) wildtype isoenzym Upp1]

Phosphate + Uridine -> D_Ribose_1_phosphate + Uracil		 (( (V * S) ) / (Km + S)) buffer: 20 mM potassium phosphate, 1 mM dithiothreitol, 1 mM EDTA
PH: 8
Temperature: 37
uniprot:Q8CGR7 [uridine phosphorylase(Enzyme) wildtype isoenzym Upp2]

Uridine + Phosphate -> D_Ribose_1_phosphate + Uracil		 (( (V * S) ) / (Km + S)) buffer: 20 mM potassium phosphate, 1 mM dithiothreitol, 1 mM EDTA
PH: 8
Temperature: 37
uniprot:Q16831 [uridine phosphorylase(Enzyme) wildtype isoenzym Upp1]

Phosphate + Doxifluridine -> 5_Deoxy_D_ribose_1_phosphate + 5_FU		 (( (V * S) ) / (Km + S)) buffer: 20 mM potassium phosphate, 1 mM dithiothreitol, 1 mM EDTA
PH: 8
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-7181 pyrimidine deoxyribonucleosides degradation
BioCyc:MOUSE_DRIBOPMET-PWY (deoxy)ribose phosphate degradation
BioCyc:HUMAN_PWY0-1295 pyrimidine ribonucleosides degradation
BioCyc:MOUSE_PWY0-163 salvage pathways of pyrimidine ribonucleotides
BioCyc:HUMAN_PWY-7181 pyrimidine deoxyribonucleosides degradation
BioCyc:META_PWY-5532 nucleoside and nucleotide degradation (archaea)
BioCyc:META_PWY-7209 superpathway of pyrimidine ribonucleosides degradation
BioCyc:ECO_PWY0-1295 pyrimidine ribonucleosides degradation
BioCyc:ECOL316407_PWY0-163 salvage pathways of pyrimidine ribonucleotides
BioCyc:ECOL316407_PWY0-1295 pyrimidine ribonucleosides degradation
BioCyc:HUMAN_PWY-7209 pyrimidine ribonucleosides degradation
BioCyc:META_PWY0-1295 pyrimidine ribonucleosides degradation
BioCyc:ECO_PWY0-163 salvage pathways of pyrimidine ribonucleotides
PathBank:SMP0002038 Pyrimidine Ribonucleosides Degradation
PathBank:SMP0000178 Dihydropyrimidinase Deficiency
PathBank:SMP0000046 Pyrimidine Metabolism
PathBank:SMP0000219 UMP Synthase Deficiency (Orotic Aciduria)
PathBank:SMP0000202 MNGIE (Mitochondrial Neurogastrointestinal Encephalopathy)
PathBank:SMP0000172 beta-Ureidopropionase Deficiency