EC: 1.7.2.1

nitrite reductase (NO-forming) (nitric-oxide:ferricytochrome-c oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P38501 [nitrite reductase (NO-forming)(Enzyme) wildtype]

Reduced_pseudoazurin + Nitrite + 2 H+ -> Oxidized_pseudoazurin + H2O + Nitric_oxide
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Mes/Hepes-NaOH
PH: 6.5
Temperature: 25
uniprot:P38501 [nitrite reductase (NO-forming)(Enzyme) wildtype]

Reduced_pseudoazurin + Nitrite + 2 H+ -> H2O + Oxidized_pseudoazurin + Nitric_oxide
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Mes/Hepes-NaOH
PH: 6.5
Temperature: 25
uniprot:P38501 [nitrite reductase (NO-forming)(Enzyme) wildtype]

Nitrite + Reduced_pseudoazurin + 2 H+ -> Oxidized_pseudoazurin + H2O + Nitric_oxide
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Mes/Hepes-NaOH
PH: 6
Temperature: 25
uniprot:P38501 [nitrite reductase (NO-forming)(Enzyme) wildtype]

2 H+ + Reduced_pseudoazurin + Nitrite -> H2O + Nitric_oxide + Oxidized_pseudoazurin
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Mes/Hepes-NaOH
PH: 6
Temperature: 25
uniprot:P38501 [nitrite reductase (NO-forming)(Enzyme) wildtype]

Nitrite + Reduced_pseudoazurin + 2 H+ -> Nitric_oxide + H2O + Oxidized_pseudoazurin
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Mes/Hepes-NaOH
PH: 7
Temperature: 25
uniprot:P38501 [nitrite reductase (NO-forming)(Enzyme) wildtype]

2 H+ + Reduced_pseudoazurin + Nitrite -> Nitric_oxide + H2O + Oxidized_pseudoazurin
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Mes/Hepes-NaOH
PH: 7.5
Temperature: 25
uniprot:P38501 [nitrite reductase (NO-forming)(Enzyme) wildtype]

Nitrite + 2 H+ + Reduced_pseudoazurin -> Nitric_oxide + Oxidized_pseudoazurin + H2O
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Mes/Hepes-NaOH
PH: 7.5
Temperature: 25
uniprot:P38501 [nitrite reductase (NO-forming)(Enzyme) wildtype]

Reduced_pseudoazurin + Nitrite + 2 H+ -> Nitric_oxide + Oxidized_pseudoazurin + H2O
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Mes/Hepes-NaOH
PH: 7
Temperature: 25
uniprot:P38501 [nitrite reductase (NO-forming)(Enzyme) wildtype]

Nitrite + Reduced_pseudoazurin + 2 H+ -> H2O + Nitric_oxide + Oxidized_pseudoazurin
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Mes/Hepes-NaOH
PH: 8
Temperature: 25
uniprot:P38501 [nitrite reductase (NO-forming)(Enzyme) wildtype]

Reduced_pseudoazurin + 2 H+ + Nitrite -> H2O + Nitric_oxide + Oxidized_pseudoazurin
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Mes/Hepes-NaOH
PH: 8
Temperature: 25
uniprot:P38501 [nitrite reductase (NO-forming)(Enzyme) wildtype]

Nitric_oxide + H2O + Oxidized_pseudoazurin -> Reduced_pseudoazurin + 2 H+ + Nitrite
(( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 100 mM Mes/Hepes-NaOH
PH: 7.5
Temperature: 25
uniprot:P38501 [nitrite reductase (NO-forming)(Enzyme) wildtype]

H2O + Oxidized_pseudoazurin + Nitric_oxide -> Nitrite + 2 H+ + Reduced_pseudoazurin
(( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 100 mM Mes/Hepes-NaOH
PH: 6.5
Temperature: 25
uniprot:P38501 [nitrite reductase (NO-forming)(Enzyme) wildtype]

H2O + Oxidized_pseudoazurin + Nitric_oxide -> 2 H+ + Reduced_pseudoazurin + Nitrite
(( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 100 mM Mes/Hepes-NaOH
PH: 7
Temperature: 25
uniprot:P38501 [nitrite reductase (NO-forming)(Enzyme) wildtype]

H2O + Nitric_oxide + Oxidized_pseudoazurin -> 2 H+ + Nitrite + Reduced_pseudoazurin
(( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 100 mM Mes/Hepes-NaOH
PH: 8
Temperature: 25

Pathways

pathway id name
BioCyc:META_DENITRIFICATION-PWY nitrate reduction I (denitrification)