EC: 1.1.1.17

mannitol-1-phosphate 5-dehydrogenase (D-mannitol-1-phosphate:NAD+ 5-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:Q4X1A4 [mannitol-1-phosphate 5-dehydrogenase(Enzyme) wildtype C-terminally his-tagged, Leu added to N-terminus]

NAD+ + D_Mannitol_1_phosphate -> NADH + D_Fructose_6_phosphate + H+		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 100 mM Tris-HCl
PH: 7.1
Temperature: 25
uniprot:Q4X1A4 [mannitol-1-phosphate 5-dehydrogenase(Enzyme) wildtype C-terminally his-tagged, Leu added to N-terminus]

D_Fructose_6_phosphate + H+ + NADH -> NAD+ + D_Mannitol_1_phosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 100 mM Tris/HCl
PH: 7.1
Temperature: 25
uniprot:Q4X1A4 [mannitol-1-phosphate 5-dehydrogenase(Enzyme) wildtype C-terminally his-tagged, Leu added to N-terminus]

NADH + H+ + D_Fructose_6_phosphate -> D_Mannitol_1_phosphate + NAD+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris/HCl
PH: 7.1
Temperature: 25
uniprot:Q4X1A4 [mannitol-1-phosphate 5-dehydrogenase(Enzyme) wildtype C-terminally his-tagged, Leu added to N-terminus]

NAD+ + D_Mannitol_1_phosphate -> D_Fructose_6_phosphate + NADH + H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.1
Temperature: 25
- [mannitol-1-phosphate 5-dehydrogenase(Enzyme) wildtype]

NADH + D_Fructose_6_phosphate -> NAD+ + D_Mannitol_1_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 5 mM Bis-Tris-propane, 3 mM Na2-EDTA, 200 mM NaCl
PH: 7
Temperature: 25
- [mannitol-1-phosphate 5-dehydrogenase(Enzyme) wildtype]

NADH + D_Fructose_6_phosphate -> D_Mannitol_1_phosphate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES-KOH, 3 mM Na2-EDTA
PH: 7
Temperature: 25
- [mannitol-1-phosphate 5-dehydrogenase(Enzyme) wildtype]

NADH + D_Fructose_6_phosphate -> NAD+ + D_Mannitol_1_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES-KOH, 3 mM Na2-EDTA
PH: 7
Temperature: 25
- [mannitol-1-phosphate 5-dehydrogenase(Enzyme) wildtype]

D_Mannitol_1_phosphate + NAD+ -> D_Fructose_6_phosphate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Bis-Tris-propane-HCl
PH: 9
Temperature: 25
- [mannitol-1-phosphate 5-dehydrogenase(Enzyme) wildtype]

NAD+ + D_Mannitol_1_phosphate -> D_Fructose_6_phosphate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Bis-Tris-propane-HCl
PH: 9
Temperature: 25
- [mannitol-1-phosphate 5-dehydrogenase(Enzyme) wildtype]

NAD+ + D_Mannitol_1_phosphate -> NADH + D_Fructose_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 5 mM Bis-Tris-propane, 100 mM NaCl
PH: 9
Temperature: 25
- [mannitol-1-phosphate 5-dehydrogenase(Enzyme) wildtype]

NADH + D_Fructose_6_phosphate -> NAD+ + D_Mannitol_1_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 5 mM Bis-Tris-propane, 3 mM Na2-EDTA, 200 mM NaCl
PH: 7
Temperature: 25
- [mannitol-1-phosphate 5-dehydrogenase(Enzyme) wildtype]

D_Mannitol_1_phosphate + NAD+ -> D_Fructose_6_phosphate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 5 mM Bis-Tris-propane, 100 mM NaCl
PH: 9
Temperature: 25
- [mannitol-1-phosphate 5-dehydrogenase(Enzyme) wildtype]

D_Fructose_6_phosphate + NADH -> NAD+ + D_Mannitol_1_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES-KOH, 3 mM Na2-EDTA
PH: 7
Temperature: 25
- [mannitol-1-phosphate 5-dehydrogenase(Enzyme) wildtype]

D_Fructose_6_phosphate + NADH -> D_Mannitol_1_phosphate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 5 mM Bis-Tris-propane, 3 mM Na2-EDTA, 200 mM NaCl
PH: 7
Temperature: 25
- [mannitol-1-phosphate 5-dehydrogenase(Enzyme) wildtype]

D_Mannitol_1_phosphate + NAD+ -> H+ + D_Fructose_6_phosphate + NADH		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM Tris-hydrochloride
PH: 8.5
Temperature: 25
- [mannitol-1-phosphate 5-dehydrogenase(Enzyme) wildtype]

D_Fructose_6_phosphate + H+ + NADH -> NAD+ + D_Mannitol_1_phosphate		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM potassium phosphate
PH: 6.2
Temperature: 25
- [mannitol-1-phosphate 5-dehydrogenase(Enzyme) wildtype]

D_Fructose_6_phosphate + H+ + NADH -> NAD+ + D_Mannitol_1_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.2
Temperature: 25
- [mannitol-1-phosphate 5-dehydrogenase(Enzyme) wildtype]

NAD+ + D_Mannitol_1_phosphate -> NADH + H+ + D_Fructose_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-hydrochloride
PH: 8.5
Temperature: 25

Pathways

pathway id name
BioCyc:BSUB_MANNIDEG-PWY mannitol degradation I
BioCyc:ECOL316407_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:META_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:ECO_MANNIDEG-PWY mannitol degradation I
BioCyc:ECO_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:ECOO157_MANNIDEG-PWY mannitol degradation I
BioCyc:ECOL316407_MANNIDEG-PWY mannitol degradation I
BioCyc:GCF_000013425_MANNIDEG-PWY mannitol degradation I
BioCyc:ECOO157_P461-PWY hexitol fermentation to lactate, formate, ethanol and acetate
BioCyc:ECOO157_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:META_P461-PWY hexitol fermentation to lactate, formate, ethanol and acetate
BioCyc:META_MANNIDEG-PWY mannitol degradation I
BioCyc:VCHO_MANNIDEG-PWY mannitol degradation I
BioCyc:PCHR_MANNIDEG-PWY mannitol degradation I
PathBank:SMP0000807 Glycolysis and Pyruvate Dehydrogenase