EC: 1.14.11.2

procollagen-proline 4-dioxygenase (procollagen-L-proline,2-oxoglutarate:oxygen oxidoreductase (4-hydroxylating))

enzyme lambda metabolic reaction experiment
- [Fe2+]

(Pro_Pro_Gly)10 + O2 + 2_Oxoglutarate -> Succinate + (Pro_4_hydroxy_Pro_Gly)10 + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/HCl, 0.1 mg/ml Catalase, 0.1 mM Dithiothreitol, 2 mg/ml Bovine serum albumin
PH: 7.8
Temperature: 37
- [5,6-O-Isopropylidene L-ascorbate]

(Pro_Pro_Gly)10 + O2 + 2_Oxoglutarate -> (Pro_4_hydroxy_Pro_Gly)10 + Succinate + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/HCl, 0.1 mg/ml Catalase, 0.1 mM Dithiothreitol, 2 mg/ml Bovine serum albumin
PH: 7.8
Temperature: 37
- [D-Isoascorbate]

(Pro_Pro_Gly)10 + O2 + 2_Oxoglutarate -> Succinate + CO2 + (Pro_4_hydroxy_Pro_Gly)10		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/HCl, 0.1 mg/ml Catalase, 0.1 mM Dithiothreitol, 2 mg/ml Bovine serum albumin
PH: 7.8
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-7894 procollagen hydroxylation and glycosylation
BioCyc:META_PWY-7894 procollagen hydroxylation and glycosylation
PathBank:SMP0120710 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0120758 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0120790 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0120569 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0000020 Arginine and Proline Metabolism
PathBank:SMP0000362 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0000188 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0000504 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0063596 Arginine and Proline Metabolism
PathBank:SMP0120721 Hyperprolinemia Type II
PathBank:SMP0120761 Prolidase Deficiency (PD)
PathBank:SMP0120789 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0120499 Hyperprolinemia Type I
PathBank:SMP0120539 Prolinemia Type II
PathBank:SMP0120572 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0000207 Prolidase Deficiency (PD)
PathBank:SMP0000208 Prolinemia Type II
PathBank:SMP0000507 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0120788 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0120501 Hyperprolinemia Type II
PathBank:SMP0120541 Prolidase Deficiency (PD)
PathBank:SMP0120570 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0000360 Hyperprolinemia Type II
PathBank:SMP0000363 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0000505 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0120675 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0120719 Hyperprolinemia Type I
PathBank:SMP0120759 Prolinemia Type II
PathBank:SMP0120791 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0120454 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0120490 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0120538 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0120571 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0120917 Succinate Signalling
PathBank:SMP0120941 Succinate Signalling
PathBank:SMP0120965 Succinate Signalling
PathBank:SMP0000361 Hyperprolinemia Type I
PathBank:SMP0000506 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0083294 Succinate Signalling
PathBank:SMP0087178 Arginine and Proline Metabolism
PathBank:SMP0087283 Arginine and Proline Metabolism