EC: 4.1.1.39

ribulose-bisphosphate carboxylase (3-phospho-D-glycerate carboxy-lyase (dimerizing; D-ribulose-1,5-bisphosphate-forming))

enzyme lambda metabolic reaction experiment
uniprot:P04718 [Mg2+]

CO2 + H2O + D_Ribulose_1,5_bisphosphate -> 2 3_Phospho_D_glycerate		 (( (( (Vmax * A) ) * B) ) / (( (( (( (KmA * KiB) ) + (KmA * B)) ) + (KmB * A)) ) + (B * A))) buffer: 100 mM Tris-HCl
PH: 8
Temperature: 30
uniprot:P04718 [Mg2+]

D_Ribulose_1,5_bisphosphate + H2O + CO2 -> 2 3_Phospho_D_glycerate		 (( (( (Vmax * A) ) * B) ) / (( (( (( (KmA * KiB) ) + (KmA * B)) ) + (KmB * A)) ) + (B * A))) buffer: 100 mM Tris-HCl
PH: 8
Temperature: 30
- [Mg2+]

CO2 + H2O + D_Ribulose_1,5_bisphosphate -> 2 3_Phospho_D_glycerate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 0.025 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
- [Mg2+]

H2O + CO2 + D_Ribulose_1,5_bisphosphate -> 2 3_Phospho_D_glycerate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 0.025 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
- [Mg2+]

D_Ribulose_1,5_bisphosphate + H2O + CO2 -> 2 3_Phospho_D_glycerate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 0.025 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
- [Mg2+]

D_Ribulose_1,5_bisphosphate + H2O + CO2 -> 2 3_Phospho_D_glycerate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 0.025 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
- [Mg2+]

H2O + CO2 + D_Ribulose_1,5_bisphosphate -> 2 3_Phospho_D_glycerate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 0.025 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
- [Mg2+]

CO2 + H2O + D_Ribulose_1,5_bisphosphate -> 2 3_Phospho_D_glycerate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 0.025 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
- [Mg2+]

H2O + CO2 + D_Ribulose_1,5_bisphosphate -> 2 3_Phospho_D_glycerate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 0.025 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
- [Mg2+]

D_Ribulose_1,5_bisphosphate + CO2 + H2O -> 2 3_Phospho_D_glycerate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 0.025 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
- [Mg2+]

H2O + D_Ribulose_1,5_bisphosphate + CO2 -> 2 3_Phospho_D_glycerate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 0.025 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
- [Mg2+]

H2O + CO2 + D_Ribulose_1,5_bisphosphate -> 2 3_Phospho_D_glycerate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 0.025 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
- [Mg2+]

H2O + D_Ribulose_1,5_bisphosphate + CO2 -> 2 3_Phospho_D_glycerate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 0.025 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
- [Mg2+]

CO2 + D_Ribulose_1,5_bisphosphate + H2O -> 2 3_Phospho_D_glycerate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 0.025 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
- [Mg2+]

D_Ribulose_1,5_bisphosphate + H2O + CO2 -> 2 3_Phospho_D_glycerate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 0.025 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
- [ribulose-bisphosphate carboxylase(Enzyme) mutant small subunit T14G]

D_Ribulose_1,5_bisphosphate + CO2 + H2O -> 2 3_Phospho_D_glycerate		 (( (kcat * (( (( (( (( (L + (x * S)) ) + Kd) ) - (( (( (L + (x * S)) ) + Kd) ) ^ 2)) ) - (( (4 * L) ) * (x * S))) ) ^ 0.5)) ) / (2 * L)) buffer: 100 mM Hepps-NaOH, 20 mM MgCl2, 1 mg/ml bovine serum albumine
PH: 7.8
Temperature: 25
- [ribulose-bisphosphate carboxylase(Enzyme) mutant small subunit Y17C]

D_Ribulose_1,5_bisphosphate + CO2 + H2O -> 2 3_Phospho_D_glycerate		 (( (kcat * (( (( (( (( (L + (x * S)) ) + Kd) ) - (( (( (L + (x * S)) ) + Kd) ) ^ 2)) ) - (( (4 * L) ) * (x * S))) ) ^ 0.5)) ) / (2 * L)) buffer: 100 mM Hepps-NaOH, 20 mM MgCl2, 1 mg/ml bovine serum albumine
PH: 7.8
Temperature: 25
- [ribulose-bisphosphate carboxylase(Enzyme) mutant small subunit T14D]

D_Ribulose_1,5_bisphosphate + CO2 + H2O -> 2 3_Phospho_D_glycerate		 (( (kcat * (( (( (( (( (L + (x * S)) ) + Kd) ) - (( (( (L + (x * S)) ) + Kd) ) ^ 2)) ) - (( (4 * L) ) * (x * S))) ) ^ 0.5)) ) / (2 * L)) buffer: 100 mM Hepps-NaOH, 20 mM MgCl2, 1 mg/ml bovine serum albumine
PH: 7.8
Temperature: 25
- [ribulose-bisphosphate carboxylase(Enzyme) wildtype]

D_Ribulose_1,5_bisphosphate + CO2 + H2O -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 100 mM Hepps-NaOH, 20 mM MgCl2, 1 mg/ml bovine serum albumine
PH: 7.8
Temperature: 25
- [ribulose-bisphosphate carboxylase(Enzyme) mutant small subunit Y17C]

D_Ribulose_1,5_bisphosphate + CO2 + H2O -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 100 mM Hepps-NaOH, 20 mM MgCl2, 1 mg/ml bovine serum albumine
PH: 7.8
Temperature: 25
- [ribulose-bisphosphate carboxylase(Enzyme) mutant small subunit T14V]

H2O + CO2 + D_Ribulose_1,5_bisphosphate -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 100 mM Hepps-NaOH, 20 mM MgCl2, 1 mg/ml bovine serum albumine
PH: 7.8
Temperature: 25
- [ribulose-bisphosphate carboxylase(Enzyme) mutant small subunit T14A]

CO2 + H2O + D_Ribulose_1,5_bisphosphate -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 100 mM Hepps-NaOH, 20 mM MgCl2, 1 mg/ml bovine serum albumine
PH: 7.8
Temperature: 25
- [ribulose-bisphosphate carboxylase(Enzyme) mutant small subunit T14G]

H2O + D_Ribulose_1,5_bisphosphate + CO2 -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 100 mM Hepps-NaOH, 20 mM MgCl2, 1 mg/ml bovine serum albumine
PH: 7.8
Temperature: 25
- [ribulose-bisphosphate carboxylase(Enzyme) mutant small subunit T14D]

CO2 + H2O + D_Ribulose_1,5_bisphosphate -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 100 mM Hepps-NaOH, 20 mM MgCl2, 1 mg/ml bovine serum albumine
PH: 7.8
Temperature: 25
- [ribulose-bisphosphate carboxylase(Enzyme) mutant small subunit T14A]

CO2 + H2O + D_Ribulose_1,5_bisphosphate -> 2 3_Phospho_D_glycerate		 (( (kcat * (( (( (( (( (L + (x * S)) ) + Kd) ) - (( (( (L + (x * S)) ) + Kd) ) ^ 2)) ) - (( (4 * L) ) * (x * S))) ) ^ 0.5)) ) / (2 * L)) buffer: 100 mM Hepps-NaOH, 20 mM MgCl2, 1 mg/ml bovine serum albumine
PH: 7.8
Temperature: 25
- [ribulose-bisphosphate carboxylase(Enzyme) wildtype]

H2O + D_Ribulose_1,5_bisphosphate + CO2 -> 2 3_Phospho_D_glycerate		 (( (kcat * (( (( (( (( (L + (x * S)) ) + Kd) ) - (( (( (L + (x * S)) ) + Kd) ) ^ 2)) ) - (( (4 * L) ) * (x * S))) ) ^ 0.5)) ) / (2 * L)) buffer: 100 mM Hepps-NaOH, 20 mM MgCl2, 1 mg/ml bovine serum albumine
PH: 7.8
Temperature: 25
- [ribulose-bisphosphate carboxylase(Enzyme) mutant small subunit T14V]

H2O + D_Ribulose_1,5_bisphosphate + CO2 -> 2 3_Phospho_D_glycerate		 (( (kcat * (( (( (( (( (L + (x * S)) ) + Kd) ) - (( (( (L + (x * S)) ) + Kd) ) ^ 2)) ) - (( (4 * L) ) * (x * S))) ) ^ 0.5)) ) / (2 * L)) buffer: 100 mM Hepps-NaOH, 20 mM MgCl2, 1 mg/ml bovine serum albumine
PH: 7.8
Temperature: 25
- [ribulose-bisphosphate carboxylase(Enzyme) mutant small subunit T14V]

CO2 + H2O + D_Ribulose_1,5_bisphosphate -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Hepps-NaOH, 20 mM MgCl2, 1 mg/ml bovine serum albumine
PH: 7.8
Temperature: 25
- [ribulose-bisphosphate carboxylase(Enzyme) mutant small subunit T14D]

H2O + CO2 + D_Ribulose_1,5_bisphosphate -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Hepps-NaOH, 20 mM MgCl2, 1 mg/ml bovine serum albumine
PH: 7.8
Temperature: 25
- [ribulose-bisphosphate carboxylase(Enzyme) wildtype]

CO2 + H2O + D_Ribulose_1,5_bisphosphate -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Hepps-NaOH, 20 mM MgCl2, 1 mg/ml bovine serum albumine
PH: 7.8
Temperature: 25
- [ribulose-bisphosphate carboxylase(Enzyme) mutant small subunit T14G]

CO2 + D_Ribulose_1,5_bisphosphate + H2O -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Hepps-NaOH, 20 mM MgCl2, 1 mg/ml bovine serum albumine
PH: 7.8
Temperature: 25
- [ribulose-bisphosphate carboxylase(Enzyme) mutant small subunit T14A]

CO2 + H2O + D_Ribulose_1,5_bisphosphate -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Hepps-NaOH, 20 mM MgCl2, 1 mg/ml bovine serum albumine
PH: 7.8
Temperature: 25
- [ribulose-bisphosphate carboxylase(Enzyme) mutant small subunit Y17C]

CO2 + D_Ribulose_1,5_bisphosphate + H2O -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Hepps-NaOH, 20 mM MgCl2, 1 mg/ml bovine serum albumine
PH: 7.8
Temperature: 25
- [ribulose-bisphosphate carboxylase(Enzyme) wildtype reduced]

CO2 + D_Ribulose_1,5_bisphosphate + H2O -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 20 mM MgCl2, 0.019 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
- [ribulose-bisphosphate carboxylase(Enzyme) wildtype oxidized]

H2O + D_Ribulose_1,5_bisphosphate + CO2 -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 20 mM MgCl2, 0.019 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
uniprot:P54205 [ribulose-bisphosphate carboxylase(Enzyme) mutant C172A reduced]

CO2 + H2O + D_Ribulose_1,5_bisphosphate -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 20 mM MgCl2, 0.019 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
uniprot:P54205 [ribulose-bisphosphate carboxylase(Enzyme) mutant C192A reduced]

H2O + CO2 + D_Ribulose_1,5_bisphosphate -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 20 mM MgCl2, 0.019 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
- [ribulose-bisphosphate carboxylase(Enzyme) wildtype reduced]

D_Ribulose_1,5_bisphosphate + CO2 + H2O -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 20 mM MgCl2, 0.019 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
- [ribulose-bisphosphate carboxylase(Enzyme) wildtype oxidized]

H2O + CO2 + D_Ribulose_1,5_bisphosphate -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 20 mM MgCl2, 0.019 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
uniprot:P54205 [ribulose-bisphosphate carboxylase(Enzyme) mutant C172A reduced]

H2O + D_Ribulose_1,5_bisphosphate + CO2 -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 20 mM MgCl2, 0.019 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
uniprot:P54205 [ribulose-bisphosphate carboxylase(Enzyme) mutant C172A-C192A reduced]

D_Ribulose_1,5_bisphosphate + H2O + CO2 -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 20 mM MgCl2, 0.019 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
uniprot:P54205 [ribulose-bisphosphate carboxylase(Enzyme) mutant C172A-C192A reduced]

H2O + D_Ribulose_1,5_bisphosphate + CO2 -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 20 mM MgCl2, 0.019 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
uniprot:P54205 [ribulose-bisphosphate carboxylase(Enzyme) mutant C192A reduced]

H2O + CO2 + D_Ribulose_1,5_bisphosphate -> 2 3_Phospho_D_glycerate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM DTT, 20 mM MgCl2, 0.019 Wilbur Anderson units/µl Carbonic anhydrase
PH: 8
Temperature: 30
- [Mg2+]

H2O + D_Ribulose_1,5_bisphosphate + CO2 -> 2 3_Phospho_D_glycerate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM EPPS
PH: 8
Temperature: 30
- [Mg2+]

H2O + D_Ribulose_1,5_bisphosphate + CO2 -> 2 3_Phospho_D_glycerate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM EPPS
PH: 8
Temperature: 30

Pathways

pathway id name
BioCyc:META_PHOTOALL-PWY oxygenic photosynthesis
BioCyc:ARA_CALVIN-PWY Calvin-Benson-Bassham cycle
BioCyc:AURANTIMONAS_CALVIN-PWY Calvin-Benson-Bassham cycle
BioCyc:SYNEL_PWY-181 photorespiration
BioCyc:META_CALVIN-PWY Calvin-Benson-Bassham cycle
BioCyc:META_PWY-181 photorespiration
BioCyc:META_PWY-7218 photosynthetic 3-hydroxybutanoate biosynthesis (engineered)
BioCyc:META_PWY-5532 nucleoside and nucleotide degradation (archaea)
BioCyc:SYNEL_PHOTOALL-PWY oxygenic photosynthesis
BioCyc:META_PWY-5723 Rubisco shunt
BioCyc:META_PWY-7124 ethylene biosynthesis V (engineered)
BioCyc:ARA_PWY-181 photorespiration
BioCyc:ARA_PWY-5723 Rubisco shunt
BioCyc:ARA_PHOTOALL-PWY oxygenic photosynthesis
BioCyc:AURANTIMONAS_PWY-5723 Rubisco shunt
BioCyc:SYNEL_PWY-5723 Rubisco shunt
BioCyc:SYNEL_CALVIN-PWY Calvin-Benson-Bassham cycle
PlantCyc:CHLAMY_PHOTOALL-PWY oxygenic photosynthesis
PlantCyc:PLANT_CALVIN-PWY Calvin-Benson-Bassham cycle
PlantCyc:ARA_PWY-5723 Rubisco shunt
PlantCyc:ARA_PWY-181 photorespiration
PlantCyc:PLANT_PWY-181 photorespiration
PlantCyc:ARA_CALVIN-PWY Calvin-Benson-Bassham cycle
PlantCyc:CHLAMY_CALVIN-PWY Calvin-Benson-Bassham cycle
PlantCyc:ARA_PHOTOALL-PWY oxygenic photosynthesis
PathBank:SMP0012094 Calvin-Benson Cycle
PathBank:SMP0012089 Photosynthesis