EC: 3.4.11.2

membrane alanyl aminopeptidase ()

enzyme lambda metabolic reaction experiment
uniprot:P15144 [membrane alanyl aminopeptidase(Enzyme) wildtype APN]

H2O + Arg_4_methylcoumaryl_7_amide -> 7_Amino_4_methylcoumarin + L_Arginine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij-35
PH: 7.5
Temperature: 37
uniprot:P0C2T8 [membrane alanyl aminopeptidase(Enzyme) wildtype]

L_Leucinyl_p_nitroanilide + H2O -> 4_Nitroaniline + L_Leucine		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM HEPES
PH: 7
Temperature: 30
uniprot:P15144 [membrane alanyl aminopeptidase(Enzyme) wildtype]

Leu_4_methylcoumaryl_7_amide + H2O -> L_Leucine + 7_Amino_4_methylcoumarin		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 0.1 % Triton-X100
PH: 8
Temperature: 37
uniprot:P04825 [membrane alanyl aminopeptidase(Enzyme) wildtype]

H2O + Leu_p_nitroanilide -> Leucine + 4_Nitroaniline		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2 % (v/v) DMSO
PH: 8
Temperature: 25
uniprot:P15145 [membrane alanyl aminopeptidase(Enzyme) wildtype]

H2O + Leu_p_nitroanilide -> 4_Nitroaniline + Leucine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 0.1 % Triton-X100
PH: 8
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-4061 glutathione-mediated detoxification I
BioCyc:META_PWY-4041 γ-glutamyl cycle
BioCyc:META_PWY-4061 glutathione-mediated detoxification I
BioCyc:CAULO_PWY-4041 γ-glutamyl cycle
BioCyc:MOUSE_PWY-4041 γ-glutamyl cycle
BioCyc:MOUSE_PWY-4061 glutathione-mediated detoxification
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-2980736 Peptide hormone metabolism
Reactome:R-HSA-2022377 Metabolism of Angiotensinogen to Angiotensins
WikiPathways:WP2729 Metabolism of Angiotensinogen to Angiotensins
PathBank:SMP0120480 gamma-Glutamyltransferase Deficiency
PathBank:SMP0120565 5-Oxoprolinase Deficiency
PathBank:SMP0000015 Glutathione Metabolism
PathBank:SMP0000500 5-Oxoprolinase Deficiency
PathBank:SMP0120485 Glutathione Synthetase Deficiency
PathBank:SMP0120566 gamma-Glutamyltranspeptidase Deficiency
PathBank:SMP0000337 Glutathione Synthetase Deficiency
PathBank:SMP0000501 gamma-Glutamyltranspeptidase Deficiency
PathBank:SMP0120446 5-Oxoprolinuria
PathBank:SMP0000143 5-Oxoprolinuria
PathBank:SMP0000183 gamma-Glutamyltransferase Deficiency
PathBank:SMP0063622 Glutathione Metabolism