EC: 3.4.11.1

leucyl aminopeptidase ()

enzyme lambda metabolic reaction experiment
uniprot:Q29571 [leucyl aminopeptidase(Enzyme) wildtype]

H2O + Arg_4_methylcoumaryl_7_amide -> 7_Amino_4_methylcoumarin + L_Arginine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij-35
PH: 7.5
Temperature: 37
uniprot:P30184 [leucyl aminopeptidase(Enzyme) wildtype]

H2O + L_Leucinyl_p_nitroanilide -> L_Leucine + 4_Nitroaniline		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 0.5 mM MnCl2
PH: 8.5
Temperature: 30
uniprot:Q10712 [Mn2+]

L_Leucylglycine + H2O -> Glycine + L_Leucine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.042 mM sodium carbonate
PH: 9.2
Temperature: 37
uniprot:Q10712 [Mn2+]

L_Leucylglycine + H2O -> L_Leucine + Glycine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.042 mM sodium carbonate
PH: 9.2
Temperature: 37
uniprot:Q10712 [Mn2+]

H2O + Arg_Leu -> L_Leucine + Arginine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.042 mM sodium carbonate
PH: 9.2
Temperature: 37
uniprot:Q10712 [Mn2+]

Glycyl_leucine + H2O -> Glycine + L_Leucine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.042 mM sodium carbonate
PH: 9.2
Temperature: 37
uniprot:P30184 [Mn2+]

L_Cysteinylglycine + H2O -> L_Cysteine + Glycine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM Tris-HCl, 50 mM NaCl
PH: 8
Temperature: 37
uniprot:P30184 [Mn2+]

H2O + Leu_Cys -> L_Cysteine + L_Leucine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM Tris-HCl, 50 mM NaCl
PH: 8
Temperature: 37
uniprot:P30184 [Mn2+]

Cys_Leu + H2O -> L_Cysteine + L_Leucine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM Tris-HCl, 50 mM NaCl
PH: 8
Temperature: 37
uniprot:Q10712 [Mn2+]

H2O + Arg_Gly -> Arginine + Glycine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.042 mM sodium carbonate
PH: 9.2
Temperature: 37
uniprot:Q10712 [Mn2+]

Leu_Leu + H2O -> 2 L_Leucine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.042 mM sodium carbonate
PH: 9.2
Temperature: 37
uniprot:Q10712 [Mn2+]

Leu_Gly_Gly + H2O -> Gly_Gly + Leucine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.042 mM sodium carbonate
PH: 9.2
Temperature: 37
uniprot:Q10712 [Mn2+]

Arg_Gly_Asp + H2O -> Arginine + Glycyl_L_aspartate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.042 mM sodium carbonate
PH: 9.2
Temperature: 37
uniprot:Q10712 [Mn2+]

Arg_Gly_Phe + H2O -> Gly_Phe + Arginine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.042 mM sodium carbonate
PH: 9.2
Temperature: 37
uniprot:Q10712 [Mn2+]

Gly_Gly + H2O -> 2 Glycine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.042 mM sodium carbonate
PH: 9.2
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-4041 γ-glutamyl cycle
BioCyc:META_PWY-4061 glutathione-mediated detoxification I
WikiPathways:WP2472 Escherichia coli K-12 peripherome