EC: 3.1.1.45

carboxymethylenebutenolidase (4-carboxymethylenebut-2-en-4-olide lactonohydrolase)

enzyme lambda metabolic reaction experiment
- [carboxymethylenebutenolidase(Enzyme) wildtype]

H2O + 4_Fluoromuconolactone -> 2_Maleylacetate + HF		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2 mM MnCl2
PH: 7.5
Temperature: 25
- [carboxymethylenebutenolidase(Enzyme) wildtype]

H2O + trans_4_Carboxymethylenebut_2_en_4_olide -> 2_Maleylacetate + HCl		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl-2 mM MnCl2
PH: 7.5
Temperature: 25

Pathways

pathway id name
BioCyc:META_14DICHLORBENZDEG-PWY 1,4-dichlorobenzene degradation
BioCyc:META_PWY-6094 3,4,6-trichlorocatechol degradation
BioCyc:META_PWY-6089 3-chlorocatechol degradation I (ortho)
BioCyc:META_PWY-6093 4,5-dichlorocatechol degradation
BioCyc:META_PWY-6084 3,5-dichlorocatechol degradation
BioCyc:META_PWY-6087 4-chlorocatechol degradation