EC: 2.7.7.72

CCA tRNA nucleotidyltransferase (CTP,CTP,ATP:tRNA cytidylyl,cytidylyl,adenylyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q58511 [CCA tRNA nucleotidyltransferase(Enzyme) wildtype his-tagged]

2 CTP + tRNA_precursor + ATP -> tRNA_with_a_3'_CCA_end + 3 Diphosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Glycine, 10-20 mM MgCl2, 1 mM DTT
PH: 9.4
Temperature: 70
uniprot:Q58511 [CCA tRNA nucleotidyltransferase(Enzyme) wildtype his-tagged]

tRNA_precursor + ATP + 2 CTP -> tRNA_with_a_3'_CCA_end + 3 Diphosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Glycine, 10-20 mM MgCl2, 1 mM DTT
PH: 9.4
Temperature: 70

Pathways

pathway id name
BioCyc:ECOL199310_PWY0-1479 tRNA processing
BioCyc:ECOO157_PWY0-1479 tRNA processing
Reactome:R-HSA-72306 tRNA processing
Reactome:R-HSA-6785470 tRNA processing in the mitochondrion
Reactome:R-HSA-8953854 Metabolism of RNA
Reactome:R-HSA-6784531 tRNA processing in the nucleus