EC: 2.3.3.5

2-methylcitrate synthase (propanoyl-CoA:oxaloacetate C-propanoyltransferase (thioester-hydrolysing, 1-carboxyethyl-forming))

enzyme lambda metabolic reaction experiment
uniprot:O34002 [2-methylcitrate synthase(Enzyme) wildtype]

Oxaloacetate + H2O + Propanoyl_CoA -> Coenzyme_A + 2_Methylcitrate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 2 mM EDTA, 100 mM KCl, 0.1 mM 5,5'-Dithiobis(2-nitrobenzoic acid)
PH: 8
Temperature: 23
uniprot:O34002 [2-methylcitrate synthase(Enzyme) mutant A361R]

H2O + Propanoyl_CoA + Oxaloacetate -> Coenzyme_A + 2_Methylcitrate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 2 mM EDTA, 100 mM KCl, 0.1 mM 5,5'-Dithiobis(2-nitrobenzoic acid)
PH: 8
Temperature: 23
uniprot:O34002 [2-methylcitrate synthase(Enzyme) wildtype]

Propanoyl_CoA + Oxaloacetate + H2O -> Coenzyme_A + 2_Methylcitrate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 2 mM EDTA, 100 mM KCl, 0.1 mM 5,5'-Dithiobis(2-nitrobenzoic acid)
PH: 8
Temperature: 23
uniprot:O34002 [2-methylcitrate synthase(Enzyme) mutant A361R]

Oxaloacetate + Propanoyl_CoA + H2O -> Coenzyme_A + 2_Methylcitrate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 2 mM EDTA, 100 mM KCl, 0.1 mM 5,5'-Dithiobis(2-nitrobenzoic acid)
PH: 8
Temperature: 23
uniprot:O34002 [2-methylcitrate synthase(Enzyme) mutant K313L/A361R/insert S315, K316, G317]

Propanoyl_CoA + Oxaloacetate + H2O -> 2_Methylcitrate + Coenzyme_A		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 2 mM EDTA, 100 mM KCl, 0.1 mM 5,5'-Dithiobis(2-nitrobenzoic acid)
PH: 8
Temperature: 23
uniprot:O34002 [2-methylcitrate synthase(Enzyme) mutant K313L/A361R/insert S315, K316, G317]

H2O + Propanoyl_CoA + Oxaloacetate -> Coenzyme_A + 2_Methylcitrate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 2 mM EDTA, 100 mM KCl, 0.1 mM 5,5'-Dithiobis(2-nitrobenzoic acid)
PH: 8
Temperature: 23

Pathways

pathway id name
BioCyc:ECOL316407_TCA TCA cycle I (prokaryotic)
BioCyc:META_PWY0-42 2-methylcitrate cycle I
BioCyc:ECO_PWY0-42 2-methylcitrate cycle I
BioCyc:ECOL316407_ANARESP1-PWY respiration (anaerobic)
BioCyc:CORYNE_GLYOXYLATE-BYPASS glyoxylate cycle
BioCyc:ECOL316407_FERMENTATION-PWY mixed acid fermentation
BioCyc:ECOL316407_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:ECOL316407_PWY0-42 2-methylcitrate cycle I
BioCyc:CORYNE_TCA TCA cycle I (prokaryotic)
BioCyc:CORYNE_FERMENTATION-PWY mixed acid fermentation
BioCyc:MTBH37RV_PWY0-42 2-methylcitrate cycle I
BioCyc:ECOL316407_GLYOXYLATE-BYPASS glyoxylate cycle
BioCyc:ECOL316407_TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
BioCyc:CORYNE_TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
PathBank:SMP0000957 Propanoate Metabolism