EC: 1.1.1.39

malate dehydrogenase (decarboxylating) ((S)-malate:NAD+ oxidoreductase (decarboxylating))

enzyme lambda metabolic reaction experiment
uniprot:P37221 [Coenzyme A]

NAD+ + L_Malate -> CO2 + Pyruvate + H+ + NADH		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 30 mM Mops, 5 mM ß-mercaptoethanol
PH: 6.9
Temperature: 25
- [Coenzyme A]

NAD+ + L_Malate -> CO2 + H+ + Pyruvate + NADH		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 30 mM Mops, 5 mM ß-mercaptoethanol
PH: 6.9
Temperature: 25
uniprot:P37221 [Mn2+]

NAD+ + L_Malate -> CO2 + NADH + H+ + Pyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM MES
PH: 6.5
Temperature: 25
uniprot:P37221 [Mg2+]

L_Malate + NAD+ -> Pyruvate + CO2 + H+ + NADH		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM MES
PH: 6.5
Temperature: 25
uniprot:P37221 [Mg2+]

NAD+ + L_Malate -> NADH + H+ + Pyruvate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM MES
PH: 6.5
Temperature: 25
uniprot:P37221 [Mg2+]

NAD+ + L_Malate -> CO2 + H+ + Pyruvate + NADH		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM MES
PH: 6.5
Temperature: 25
uniprot:P37221 [Mg2+]

NADP+ + L_Malate -> Pyruvate + H+ + CO2 + NADPH		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM MES
PH: 6.5
Temperature: 25
uniprot:P37221 [Mg2+]

NADP+ + L_Malate -> NADPH + CO2 + H+ + Pyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM MES
PH: 6.5
Temperature: 25
uniprot:P37221 [Mg2+]

L_Malate + NADP+ -> H+ + NADPH + Pyruvate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM MES
PH: 6.5
Temperature: 25
uniprot:P37221 [Mn2+]

NAD+ + L_Malate -> H+ + CO2 + Pyruvate + NADH		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM MES
PH: 6.5
Temperature: 25
uniprot:P37221 [Mn2+]

L_Malate + NAD+ -> Pyruvate + CO2 + H+ + NADH		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM MES
PH: 6.5
Temperature: 25
uniprot:P37221 [Mn2+]

NADP+ + L_Malate -> H+ + Pyruvate + NADPH + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM MES
PH: 6.5
Temperature: 25
uniprot:P37221 [Mn2+]

L_Malate + NADP+ -> NADPH + Pyruvate + CO2 + H+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM MES
PH: 6.5
Temperature: 25
uniprot:P37221 [Mn2+]

L_Malate + NADP+ -> H+ + NADPH + Pyruvate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM MES
PH: 6.5
Temperature: 25
- [malate dehydrogenase (decarboxylating)(Enzyme) wildtype ME 39]

NAD+ + L_Malate -> NADH + Pyruvate + H+ + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MnCl2
PH: 7.5
Temperature: 25
- [malate dehydrogenase (decarboxylating)(Enzyme) wildtype ME 39]

L_Malate + NADP+ -> Pyruvate + H+ + NADPH + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MnCl2
PH: 7.5
Temperature: 25
uniprot:E2CWD2 [malate dehydrogenase (decarboxylating)(Enzyme) wildtype LsME1]

NAD+ + L_Malate -> Pyruvate + H+ + CO2 + NADH		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM HEPES, 5 mM MnCl2
PH: 7.3
Temperature: 28
uniprot:E2CWD2 [malate dehydrogenase (decarboxylating)(Enzyme) wildtype LsME1]

L_Malate + NAD+ -> NADH + Pyruvate + CO2 + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM MnCl2
PH: 7.3
Temperature: 28
uniprot:E2CWD2 [malate dehydrogenase (decarboxylating)(Enzyme) wildtype LsME1]

L_Malate + NADP+ -> CO2 + H+ + NADPH + Pyruvate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM HEPES, 5 mM MnCl2
PH: 7.3
Temperature: 28
uniprot:E2CWD2 [malate dehydrogenase (decarboxylating)(Enzyme) wildtype LsME1]

L_Malate + NADP+ -> Pyruvate + NADPH + H+ + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES, 5 mM MnCl2
PH: 7.3
Temperature: 28
- [Mn2+]

L_Malate + NAD+ -> CO2 + NADH + Pyruvate + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes/KOH, 0.5 mM dithiothreitol, 1 mM EDTA/Na4
PH: 7.4
Temperature: 30
- [Mn2+]

NAD+ + L_Malate -> NADH + H+ + CO2 + Pyruvate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Hepes/KOH, 0.5 mM dithiothreitol, 1 mM EDTA/Na4
PH: 7.4
Temperature: 30
- [Mn2+]

NADP+ + L_Malate -> CO2 + Pyruvate + H+ + NADPH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes/KOH, 0.5 mM dithiothreitol, 1 mM EDTA/Na4
PH: 7.4
Temperature: 30
- [Mn2+]

L_Malate + NADP+ -> CO2 + Pyruvate + NADPH + H+		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Hepes/KOH, 0.5 mM dithiothreitol, 1 mM EDTA/Na4
PH: 7.4
Temperature: 30
- [Mn2+]

L_Malate + NAD+ -> CO2 + H+ + NADH + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes/KOH, 0.5 mM dithiothreitol, 1 mM EDTA/Na4
PH: 7.4
Temperature: 30
- [Mn2+]

NAD+ + L_Malate -> CO2 + NADH + Pyruvate + H+		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Hepes/KOH, 0.5 mM dithiothreitol, 1 mM EDTA/Na4
PH: 7.4
Temperature: 30
- [Mn2+]

NADP+ + L_Malate -> H+ + CO2 + NADPH + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes/KOH, 0.5 mM dithiothreitol, 1 mM EDTA/Na4
PH: 7.4
Temperature: 30
- [Mn2+]

L_Malate + NADP+ -> CO2 + H+ + NADPH + Pyruvate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Hepes/KOH, 0.5 mM dithiothreitol, 1 mM EDTA/Na4
PH: 7.4
Temperature: 30
uniprot:P23368 [Mg2+]

L_Malate + NAD+ -> Pyruvate + CO2 + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

NAD+ + L_Malate -> Pyruvate + CO2 + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

L_Malate + NAD+ -> CO2 + NADH + Pyruvate		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

L_Malate + NAD+ -> NADH + Pyruvate + CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

L_Malate + NAD+ -> Pyruvate + NADH + CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

NAD+ + L_Malate -> CO2 + NADH + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Mg2+]

NAD+ + L_Malate -> NADH + Pyruvate + CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Mg2+]

NAD+ + L_Malate -> Pyruvate + CO2 + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Mg2+]

L_Malate + NAD+ -> NADH + CO2 + Pyruvate		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Mg2+]

NAD+ + L_Malate -> Pyruvate + NADH + CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

L_Malate + NAD+ -> Pyruvate + CO2 + NADH		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

L_Malate + NAD+ -> CO2 + Pyruvate + NADH		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

NAD+ + L_Malate -> NADH + CO2 + Pyruvate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

NAD+ + L_Malate -> Pyruvate + NADH + CO2		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Mg2+]

NAD+ + L_Malate -> Pyruvate + NADH + CO2		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Mg2+]

NAD+ + L_Malate -> CO2 + NADH + Pyruvate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Mg2+]

NAD+ + L_Malate -> Pyruvate + NADH + CO2		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Mg2+]

L_Malate + NAD+ -> CO2 + NADH + Pyruvate		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Mg2+]

L_Malate + NAD+ -> Pyruvate + NADH + CO2		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

L_Malate + NAD+ -> NADH + CO2 + Pyruvate		 (( (( (( (E * kcat) ) * A) ) / (Km + A)) ) + (( (( (E * kcat_act) ) * Z) ) / (Kact + Z))) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

L_Malate + NAD+ -> Pyruvate + CO2 + NADH		 (( (( (( (E * kcat) ) * A) ) / (Km + A)) ) + (( (( (E * kcat_act) ) * Z) ) / (Kact + Z))) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

L_Malate + NAD+ -> CO2 + Pyruvate + NADH		 (( (( (( (E * kcat) ) * A) ) / (Km + A)) ) + (( (( (E * kcat_act) ) * Z) ) / (Kact + Z))) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

L_Malate + NAD+ -> CO2 + Pyruvate + NADH		 (( (( (( (E * kcat) ) * A) ) / (Km + A)) ) + (( (( (E * kcat_act) ) * Z) ) / (Kact + Z))) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

L_Malate + NAD+ -> Pyruvate + NADH + CO2		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

NAD+ + L_Malate -> NADH + Pyruvate + CO2		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

L_Malate + NAD+ -> Pyruvate + NADH + CO2		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Mg2+]

NAD+ + L_Malate -> NADH + Pyruvate + CO2		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Mg2+]

NAD+ + L_Malate -> NADH + Pyruvate + CO2		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

L_Malate + NAD+ -> Pyruvate + NADH + CO2		 (( (( (( (E * kcat) ) * C) ) / (Km + C)) ) + (( (( (E * kcat_act) ) * Z) ) / (Kact + Z))) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

L_Malate + NAD+ -> CO2 + NADH + Pyruvate		 (( (( (( (E * kcat) ) * C) ) / (Km + C)) ) + (( (( (E * kcat_act) ) * Z) ) / (Kact + Z))) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

L_Malate + NAD+ -> NADH + Pyruvate + CO2		 (( (( (( (E * kcat) ) * A) ) / (Km + A)) ) + (( (( (E * kcat_act) ) * Z) ) / (Kact + Z))) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

NAD+ + L_Malate -> Pyruvate + CO2 + NADH		 (( (( (( (E * kcat) ) * A) ) / (Km + A)) ) + (( (( (E * kcat_act) ) * Z) ) / (Kact + Z))) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

NAD+ + L_Malate -> Pyruvate + NADH + CO2		 (( (( (( (E * kcat) ) * C) ) / (Km + C)) ) + (( (( (E * kcat_act) ) * Z) ) / (Kact + Z))) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

NAD+ + L_Malate -> Pyruvate + CO2 + NADH		 (( (( (( (E * kcat) ) * C) ) / (Km + C)) ) + (( (( (E * kcat_act) ) * Z) ) / (Kact + Z))) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

L_Malate + NAD+ -> CO2 + Pyruvate + NADH		 (( (( (( (E * kcat) ) * B) ) / (Km + B)) ) + (( (( (E * kcat_act) ) * Z) ) / (Kact + Z))) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

NAD+ + L_Malate -> Pyruvate + NADH + CO2		 (( (( (( (E * kcat) ) * A) ) / (Km + A)) ) + (( (( (E * kcat_act) ) * Z) ) / (Kact + Z))) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

L_Malate + NAD+ -> Pyruvate + CO2 + NADH		 (( (( (( (E * kcat) ) * A) ) / (Km + A)) ) + (( (( (E * kcat_act) ) * Z) ) / (Kact + Z))) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

NAD+ + L_Malate -> NADH + CO2 + Pyruvate		 (( (( (( (E * kcat) ) * B) ) / (Km + B)) ) + (( (( (E * kcat_act) ) * Z) ) / (Kact + Z))) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

L_Malate + NAD+ -> Pyruvate + NADH + CO2		 (( (( (( (E * kcat) ) * B) ) / (Km + B)) ) + (( (( (E * kcat_act) ) * Z) ) / (Kact + Z))) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30
uniprot:P23368 [Fumarate]

NAD+ + L_Malate -> CO2 + NADH + Pyruvate		 (( (( (( (E * kcat) ) * B) ) / (Km + B)) ) + (( (( (E * kcat_act) ) * Z) ) / (Kact + Z))) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 30

Pathways

pathway id name
BioCyc:ARA_PWYQT-4481 TCA cycle variation V (plant)
BioCyc:ARA_GLUCONEO-PWY gluconeogenesis I
PlantCyc:ARA_GLUCONEO-PWY gluconeogenesis I
PlantCyc:PLANT_PWYQT-4481 TCA cycle variation V (plant)
PlantCyc:ARA_PWYQT-4481 TCA cycle variation V (plant)
WikiPathways:WP2624 TCA cycle (Krebs cycle)
PathBank:SMP0002413 Gluconeogenesis from L-Malic Acid
PathBank:SMP0002414 TCA Cycle