EC: 1.5.1.20

methylenetetrahydrofolate reductase [NAD(P)H] (5-methyltetrahydrofolate:NAD(P)+ oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P0AEZ1 [FAD]

Menadione + H+ + NADH -> Menadiol + NAD+		 (( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (( (Kmb * A) ) * (1 + (A / Kia))) ) + (Kma * B)) ) + (A * B))) buffer: 50 mM potassium phosphate, 0.3 mM EDTA, 10% glycerol
PH: 7.2
Temperature: 25
uniprot:P0AEZ1 [FAD]

NADH + Menadione + H+ -> Menadiol + NAD+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA, 10% glycerol
PH: 7.2
Temperature: 25
uniprot:P0AEZ1 [FAD]

5_Methyltetrahydrofolate + Menadione -> Menadiol + 5,10_Methylenetetrahydrofolate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA, 10% glycerol
PH: 7.2
Temperature: 25
uniprot:P0AEZ1 [FAD]

H+ + 5,10_Methylenetetrahydrofolate + NADH -> 5_Methyltetrahydrofolate + NAD+		 (( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (( (Kma * B) ) * (1 + (B / Kib))) ) + (( (Kmb * A) ) * (1 + (A / Kia)))) ) + (A * B))) buffer: 50 mM potassium phosphate, 0.3 mM EDTA, 10% glycerol
PH: 7.2
Temperature: 25
uniprot:P0AEZ1 [FAD]

Menadione + H+ + NADH -> Menadiol + NAD+		 (( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (( (Kmb * A) ) * (1 + (A / Kia))) ) + (Kma * B)) ) + (A * B))) buffer: 50 mM potassium phosphate, 0.3 mM EDTA, 10% glycerol
PH: 7.2
Temperature: 25
uniprot:P0AEZ1 [FAD]

Menadione + 5_Methyltetrahydrofolate -> 5,10_Methylenetetrahydrofolate + Menadiol		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA, 10% glycerol, 0.24% bovine serum albumin
PH: 7.2
Temperature: 25

Pathways

pathway id name
BioCyc:BSUB_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:BSUB_METSYN-PWY superpathway of L-homoserine and L-methionine biosynthesis
BioCyc:YEAST_PWY3O-697 folate interconversions
BioCyc:BSUB_HOMOSER-METSYN-PWY L-methionine biosynthesis I
BioCyc:YEAST_PWY-2201 folate transformations
BioCyc:BSUB_MET-SAM-PWY superpathway of S-adenosyl-L-methionine biosynthesis
BioCyc:BSUB_PWY-2201 folate transformations I
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-196854 Metabolism of vitamins and cofactors
Reactome:R-SPO-196757 Metabolism of folate and pterines
Reactome:R-SPO-196849 Metabolism of water-soluble vitamins and cofactors