EC: 1.1.1.292

1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) (1,5-anhydro-D-mannitol:NADP+ oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:Q2I8V6 [1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming)(Enzyme) wildtype His6-tagged]

NADPH + H+ + 1,5_Anhydro_D_fructose -> 1,5_Anhydro_D_mannitol + NADP+
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Bistris-HCl
PH: 6.5
Temperature: 30
uniprot:Q2I8V6 [1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming)(Enzyme) wildtype His6-tagged]

NADPH + D_Glucosone + H+ -> D_Mannose + NADP+
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Bistris-HCl
PH: 6.5
Temperature: 30
uniprot:Q2I8V6 [1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming)(Enzyme) wildtype His6-tagged]

H+ + NADPH + 1,5_Anhydro_D_fructose -> NADP+ + 1,5_Anhydro_D_mannitol
(( (( (kcat * E) ) * S2) ) / (Km + S2)) buffer: 100 mM Bistris-HCl
PH: 6.5
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-6992 1,5-anhydrofructose degradation