EC: 1.4.1.13

glutamate synthase (NADPH) (L-glutamate:NADP+ oxidoreductase (transaminating))

enzyme lambda metabolic reaction experiment
uniprot:P39812 [glutamate synthase (NADPH)(Enzyme) wildtype]

L_Glutamine + NADPH + 2_Oxoglutarate + H+ -> 2 L_Glutamate + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.8
Temperature: 30
uniprot:P39812 [glutamate synthase (NADPH)(Enzyme) wildtype]

NADPH + 2_Oxoglutarate + L_Glutamine + H+ -> 2 L_Glutamate + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.8
Temperature: 30
uniprot:P39812 [glutamate synthase (NADPH)(Enzyme) wildtype]

L_Glutamine + 2_Oxoglutarate + H+ + NADPH -> NADP+ + 2 L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.8
Temperature: 30
uniprot:P39812 [glutamate synthase (NADPH)(Enzyme) wildtype]

2_Oxoglutarate + NADPH + H+ + L_Glutamine -> 2 L_Glutamate + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.8
Temperature: 30
uniprot:P39812 [glutamate synthase (NADPH)(Enzyme) wildtype]

L_Glutamine + H+ + 2_Oxoglutarate + NADPH -> 2 L_Glutamate + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.8
Temperature: 30
uniprot:P39812 [glutamate synthase (NADPH)(Enzyme) wildtype]

2_Oxoglutarate + L_Glutamine + NADPH + H+ -> NADP+ + 2 L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.8
Temperature: 30
uniprot:Q05756 [ADPribose 2'-phosphate]

Iodonitrotetrazolium + H+ + NADPH -> NADP+ + Reduced_iodonitrotetrazolium		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM Hepes/KOH
PH: 7.5
Temperature: 25
uniprot:Q05756 [ADPribose 2'-phosphate]

NADPH + H+ + Iodonitrotetrazolium -> NADP+ + Reduced_iodonitrotetrazolium		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM Hepes/KOH
PH: 7.5
Temperature: 25
uniprot:Q05756 [3-Aminopyridine adenine dinucleotide phosphate]

Iodonitrotetrazolium + H+ + NADPH -> Reduced_iodonitrotetrazolium + NADP+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM Hepes/KOH
PH: 7.5
Temperature: 25
uniprot:Q05756 [3-Aminopyridine adenine dinucleotide phosphate]

H+ + NADPH + Iodonitrotetrazolium -> Reduced_iodonitrotetrazolium + NADP+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM Hepes/KOH
PH: 7.5
Temperature: 25
uniprot:Q05756 [NADP+]

Iodonitrotetrazolium + H+ + NADPH -> NADP+ + Reduced_iodonitrotetrazolium		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM Hepes/KOH
PH: 7.5
Temperature: 25
uniprot:Q05756 [glutamate synthase (NADPH)(Enzyme) mutant G298A]

H+ + NADPH + Iodonitrotetrazolium -> NADP+ + Reduced_iodonitrotetrazolium		 (( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (Km_A * B) ) + (Km_B * A)) ) + (A * B))) buffer: 50 mM Hepes/KOH
PH: 7.5
Temperature: 25
- [glutamate synthase (NADPH)(Enzyme) wildtype]

H+ + 2_Oxoglutarate + L_Glutamine + NADPH -> NADP+ + 2 L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
- [glutamate synthase (NADPH)(Enzyme) wildtype]

2_Oxoglutarate + H+ + NADPH + L_Glutamine -> NADP+ + 2 L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25
- [glutamate synthase (NADPH)(Enzyme) wildtype]

H+ + L_Glutamine + NADPH + 2_Oxoglutarate -> 2 L_Glutamate + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY-5505 L-glutamate and L-glutamine biosynthesis
BioCyc:MTBH37RV_GLUTAMINDEG-PWY L-glutamine degradation I
BioCyc:MTBCDC1551_GLUTAMINEFUM-PWY L-glutamine degradation II
BioCyc:MTBCDC1551_AMMASSIM-PWY ammonia assimilation cycle III
BioCyc:MTBCDC1551_GLUTAMINDEG-PWY L-glutamine degradation I
BioCyc:BSUB_AMMASSIM-PWY ammonia assimilation cycle III
BioCyc:BSUB_GLUTSYN-PWY L-glutamate biosynthesis I
BioCyc:MOB3B_GLUTAMINEFUM-PWY glutamine degradation II
BioCyc:META_AMMASSIM-PWY ammonia assimilation cycle III
BioCyc:ECO_GLUTSYN-PWY L-glutamate biosynthesis I
BioCyc:MTBCDC1551_GLUTSYN-PWY L-glutamate biosynthesis I
BioCyc:BSUB_GLUTAMINEFUM-PWY L-glutamine degradation II
BioCyc:ECOL316407_GLUTSYNIII-PWY glutamate biosynthesis III
BioCyc:ECOL316407_AMMASSIM-PWY ammonia assimilation cycle III
BioCyc:MOB3B_GLUTSYN-PWY glutamate biosynthesis I
BioCyc:ECO_AMMASSIM-PWY ammonia assimilation cycle III
BioCyc:ECOL316407_GLUTSYN-PWY glutamate biosynthesis I
BioCyc:MOB3B_AMMASSIM-PWY superpathway of glutamate biosynthesis
BioCyc:META_GLUTAMINEFUM-PWY L-glutamine degradation II
BioCyc:META_GLUTSYN-PWY L-glutamate biosynthesis I
BioCyc:MTBCDC1551_GLUTSYNIII-PWY L-glutamate biosynthesis III
PathBank:SMP0000778 Nitrogen Metabolism
PathBank:SMP0000811 L-Glutamate Metabolism