EC: 1.3.99.4

3-oxosteroid 1-dehydrogenase (3-oxosteroid:acceptor DELTA1-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P71864 [FAD]

2,6_Dichloroindophenol + 5alpha_Androstane_17beta_ol_3_one -> 5alpha_Androst_1_en_17beta_ol_3_one + Reduced_2,6_dichlorophenolindophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:P71864 [FAD]

2,6_Dichloroindophenol + 5alpha_Androstane_3,17_dione -> Reduced_2,6_dichlorophenolindophenol + 5alpha_Androst_1_ene_3,17_dione		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:A9QAE5 [FAD]

2,6_Dichloroindophenol + 5alpha_Androstane_3,17_dione -> Reduced_2,6_dichlorophenolindophenol + 5alpha_Androst_1_ene_3,17_dione		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:A9QAE5 [FAD]

5alpha_Pregnane_3,20_dione + 2,6_Dichloroindophenol -> 5alpha_Pregn_1_ene_3,20_dione + Reduced_2,6_dichlorophenolindophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:P71864 [FAD]

Progesterone + 2,6_Dichloroindophenol -> Reduced_2,6_dichlorophenolindophenol + 1_Dehydroprogesterone		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:A9QAE5 [FAD]

2,6_Dichloroindophenol + Progesterone -> 1_Dehydroprogesterone + Reduced_2,6_dichlorophenolindophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:Q9RA02 [FAD]

2,6_Dichloroindophenol + 5alpha_Androstane_17beta_ol_3_one -> Reduced_2,6_dichlorophenolindophenol + 5alpha_Androst_1_en_17beta_ol_3_one		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:Q9RA02 [FAD]

5alpha_Androstane_3,17_dione + 2,6_Dichloroindophenol -> 5alpha_Androst_1_ene_3,17_dione + Reduced_2,6_dichlorophenolindophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:Q9RA02 [FAD]

2,6_Dichloroindophenol + Androst_4_ene_3,17_dione -> 1,4_Androstdiene_3,17_dione + Reduced_2,6_dichlorophenolindophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:Q9RA02 [FAD]

2,6_Dichloroindophenol + Cortisol -> Reduced_2,6_dichlorophenolindophenol + 11beta,17,21_Trihydroxypregn_1,4_diene_3,20_dione		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:Q9RA02 [FAD]

2,6_Dichloroindophenol + 22,23_Bisnor_5alpha_cholanic_acid_3_one -> Reduced_2,6_dichlorophenolindophenol + 22,23_Bisnor_5alpha_1_cholenic_acid_3_one		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:Q9RA02 [FAD]

2,6_Dichloroindophenol + 5alpha_Pregnane_3,20_dione -> 5alpha_Pregn_1_ene_3,20_dione + Reduced_2,6_dichlorophenolindophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:Q9RA02 [FAD]

2,6_Dichloroindophenol + 9alpha_Hydroxy_4_androstene_3,17_dione -> 9alpha_Hydroxy_androst_1,4_diene_3,17_dione + Reduced_2,6_dichlorophenolindophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:Q9RA02 [FAD]

Progesterone + 2,6_Dichloroindophenol -> 1_Dehydroprogesterone + Reduced_2,6_dichlorophenolindophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:Q8RLT5 [FAD]

2,6_Dichloroindophenol + 5alpha_Androstane_17beta_ol_3_one -> 5alpha_Androst_1_en_17beta_ol_3_one + Reduced_2,6_dichlorophenolindophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:A9QAE5 [FAD]

2,6_Dichloroindophenol + 5alpha_Androstane_17beta_ol_3_one -> 5alpha_Androst_1_en_17beta_ol_3_one + Reduced_2,6_dichlorophenolindophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:Q8RLT5 [FAD]

2,6_Dichloroindophenol + 5alpha_Androstane_3,17_dione -> Reduced_2,6_dichlorophenolindophenol + 5alpha_Androst_1_ene_3,17_dione		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:Q8RLT5 [FAD]

2,6_Dichloroindophenol + Cortisol -> 11beta,17,21_Trihydroxypregn_1,4_diene_3,20_dione + Reduced_2,6_dichlorophenolindophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:Q8RLT5 [FAD]

2,6_Dichloroindophenol + 22,23_Bisnor_5alpha_cholanic_acid_3_one -> 22,23_Bisnor_5alpha_1_cholenic_acid_3_one + Reduced_2,6_dichlorophenolindophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:Q8RLT5 [FAD]

2,6_Dichloroindophenol + 5alpha_Pregnane_3,20_dione -> Reduced_2,6_dichlorophenolindophenol + 5alpha_Pregn_1_ene_3,20_dione		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:Q8RLT5 [FAD]

2,6_Dichloroindophenol + 9alpha_Hydroxy_4_androstene_3,17_dione -> Reduced_2,6_dichlorophenolindophenol + 9alpha_Hydroxy_androst_1,4_diene_3,17_dione		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:Q8RLT5 [FAD]

Progesterone + 2,6_Dichloroindophenol -> 1_Dehydroprogesterone + Reduced_2,6_dichlorophenolindophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30
uniprot:Q8RLT5 [FAD]

4_Androstane_3,17_dione + 2,6_Dichloroindophenol -> Reduced_2,6_dichlorophenolindophenol + 1,4_Androstdiene_3,17_dione		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 7.4
Temperature: 30

Pathways

pathway id name
BioCyc:MTBCDC1551_PWY-6937 superpathway of testosterone and androsterone degradation
BioCyc:META_PWY-6944 androstenedione degradation
BioCyc:MTBH37RV_PWY-6947 superpathway of cholesterol degradation II (cholesterol dehydrogenase)
BioCyc:MTBCDC1551_PWY-6947 superpathway of cholesterol degradation II (cholesterol dehydrogenase)
BioCyc:META_PWY-6947 superpathway of cholesterol degradation II (cholesterol dehydrogenase)
BioCyc:MTBH37RV_PWY-6944 androstenedione degradation
BioCyc:MTBCDC1551_PWY-6944 androstenedione degradation