EC: 1.14.13.225

F-actin monooxygenase ([F-actin]-L-methionine,NADPH:O2 S-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:Q8TDZ2 [FAD]

H+ + O2 + NADPH -> H2O2 + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

O2 + NADPH + H+ -> H2O2 + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Hepes/KOH, 0.1 M NaCl
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

H+ + O2 + NADPH -> H2O2 + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

H+ + NADPH + O2 -> NADP+ + H2O2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

O2 + H+ + NADPH -> H2O2 + NADP+		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (m * u))) ) + (S * (1 + (n * u))))) buffer: 20 mM Hepes/NaOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [Epigallocatechin 3-gallate]

O2 + H+ + NADPH -> NADP+ + H2O2		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / Ki))))) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

H+ + NADPH + O2 -> H2O2 + NADP+		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM Hepes/KOH, 0.1 M NaCl
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

O2 + H+ + NADPH -> H2O2 + NADP+		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

H+ + O2 + NADPH -> NADP+ + H2O2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Hepes/KOH, 10% Glycerol
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

O2 + H+ + NADPH -> H2O2 + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

NADPH + H+ + O2 -> NADP+ + H2O2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

[F_actin]_L_methionine + O2 + H+ + NADPH -> H2O + NADP+ + [F_actin]_L_methionine_(R)_S_oxide		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

H+ + NADH + O2 -> NAD+ + H2O2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

H+ + O2 + NADPH -> NADP+ + H2O2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 9 mM Tris/HCl, 0.18 mM CaCl2, 1.1 mM ATP, 1.3 mM DTT, 45 mM KCl, 1.8 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q8TDZ2 [FAD]

NADPH + H+ + O2 -> H2O2 + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

NADPH + H+ + O2 -> H2O2 + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 5 mM Tris/HCl, 0.2 mM CaCl2, 0.2 mM ATP, 1 mM DTT
PH: 8
Temperature: 25
uniprot:Q8TDZ2 [FAD]

[F_actin]_L_methionine + NADPH + H+ + O2 -> [F_actin]_L_methionine_(R)_S_oxide + H2O + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 9 mM Tris/HCl, 0.18 mM CaCl2, 1.1 mM ATP, 1.3 mM DTT, 45 mM KCl, 1.8 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q8TDZ2 [FAD]

[F_actin]_L_methionine + O2 + NADPH + H+ -> NADP+ + [F_actin]_L_methionine_(R)_S_oxide + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 9 mM Tris/HCl, 0.18 mM CaCl2, 1.1 mM ATP, 1.3 mM DTT, 45 mM KCl, 1.8 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q8TDZ2 [FAD]

[F_actin]_L_methionine + H+ + NADH + O2 -> NAD+ + [F_actin]_L_methionine_(R)_S_oxide + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

[G_actin]_L_methionine + H+ + NADPH + O2 -> [G_actin]_L_methionine_(R)_S_oxide + NADP+ + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 5 mM Tris/HCl, 0.2 mM CaCl2, 0.2 mM ATP, 1 mM DTT
PH: 8
Temperature: 25
uniprot:Q8TDZ2 [FAD]

H+ + NADH + O2 -> NAD+ + H2O2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 5 mM Tris/HCl, 0.2 mM CaCl2, 0.2 mM ATP, 1 mM DTT
PH: 8
Temperature: 25
uniprot:Q8TDZ2 [FAD]

NADH + O2 + H+ -> H2O2 + NAD+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 9 mM Tris/HCl, 0.18 mM CaCl2, 1.1 mM ATP, 1.3 mM DTT, 45 mM KCl, 1.8 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q8TDZ2 [FAD]

NADPH + O2 + H+ -> NADP+ + H2O2		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

H+ + NADPH + O2 -> NADP+ + H2O2		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [Benzoate]

O2 + NADPH + H+ -> H2O2 + NADP+		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

O2 + NADPH + H+ -> H2O2 + NADP+		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

NADPH + H+ + O2 -> NADP+ + H2O2		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [DL-Dopa]

H+ + NADPH + O2 -> NADP+ + H2O2		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

[F_actin]_L_methionine + H+ + NADH + O2 -> [F_actin]_L_methionine_(R)_S_oxide + NAD+ + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 9 mM Tris/HCl, 0.18 mM CaCl2, 1.1 mM ATP, 1.3 mM DTT, 45 mM KCl, 1.8 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q8TDZ2 [FAD]

[G_actin]_L_methionine + O2 + NADH + H+ -> [G_actin]_L_methionine_(R)_S_oxide + NAD+ + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 5 mM Tris/HCl, 0.2 mM CaCl2, 0.2 mM ATP, 1 mM DTT
PH: 8
Temperature: 25
uniprot:Q8TDZ2 [FAD]

NADH + H+ + O2 -> H2O2 + NAD+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [2,5-Dihydroxybenzoate]

O2 + NADPH + H+ -> H2O2 + NADP+		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

NADPH + H+ + O2 -> H2O2 + NADP+		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [4-Aminobenzoate]

H+ + NADPH + O2 -> H2O2 + NADP+		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [4-Hydroxybenzoate]

H+ + O2 + NADPH -> NADP+ + H2O2		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

NADPH + O2 + H+ -> NADP+ + H2O2		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [3-Hydroxybenzoate]

H+ + O2 + NADPH -> NADP+ + H2O2		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [2,4,6-Trihydroxybenzoate]

H+ + NADPH + O2 -> NADP+ + H2O2		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [2-Benzylbenzoate]

O2 + NADPH + H+ -> H2O2 + NADP+		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [4-Benzylbenzoate]

NADPH + H+ + O2 -> H2O2 + NADP+		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [2,3-Dihydroxybenzoate]

H+ + NADPH + O2 -> H2O2 + NADP+		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Kis))) ) + (S * (1 + (I / Kii))))) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [3-Chlorobenzoate]

NADPH + H+ + O2 -> NADP+ + H2O2		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Kis))) ) + (S * (1 + (I / Kii))))) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

H+ + NADPH + O2 -> H2O2 + NADP+		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Kis))) ) + (S * (1 + (I / Kii))))) buffer: 20 mM Hepes/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

[F_actin]_L_methionine + O2 + NADPH + H+ -> H2O + NADP+ + [F_actin]_L_methionine_(R)_S_oxide		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 9.5 mM Tris/HCl, 45 mM KCl, 0.18 mM CaCl2, 0.18 mM MgCl, 1.1 mM ATP, 1.3 mM DTT, 1 mg/ml catalase
PH: 7.7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

NADPH + [F_actin]_L_methionine + H+ + O2 -> H2O + [F_actin]_L_methionine_(R)_S_oxide + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 9.5 mM Tris/HCl, 45 mM KCl, 0.18 mM CaCl2, 0.18 mM MgCl, 1.1 mM ATP, 1.3 mM DTT, 1 mg/ml catalase
PH: 7.7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

NADPH + O2 + H+ + [F_actin]_L_methionine -> [F_actin]_L_methionine_(R)_S_oxide + H2O + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 9.5 mM Tris/HCl, 45 mM KCl, 0.18 mM CaCl2, 0.18 mM MgCl, 1.1 mM ATP, 1.3 mM DTT, 1 mg/ml catalase
PH: 7.7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

O2 + NADPH + H+ -> H2O2 + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM HEPES/KOH
PH: 7
Temperature: 25
uniprot:Q8VDP3 [FAD]

H+ + O2 + NADPH -> NADP+ + H2O2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM HEPES, 100 mM NaCl
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

H+ + NADPH + O2 -> NADP+ + H2O2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 9.5 mM Tris/HCl, 45 mM KCl, 0.18 mM CaCl2, 0.18 mM MgCl, 1.1 mM ATP, 1.3 mM DTT, 1 mg/ml catalase
PH: 7.7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

O2 + H+ + NADPH -> H2O2 + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM HEPES/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

H+ + O2 + NADPH -> H2O2 + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 9.5 mM Tris/HCl, 45 mM KCl, 0.18 mM CaCl2, 0.18 mM MgCl, 1.1 mM ATP, 1.3 mM DTT, 1 mg/ml catalase
PH: 7.7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

NADPH + H+ + O2 -> H2O2 + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 9.5 mM Tris/HCl, 45 mM KCl, 0.18 mM CaCl2, 0.18 mM MgCl, 1.1 mM ATP, 1.3 mM DTT, 1 mg/ml catalase
PH: 7.7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

O2 + NADPH + H+ -> NADP+ + H2O2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM HEPES/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

O2 + NADPH + H+ -> NADP+ + H2O2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM HEPES/KOH
PH: 7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

O2 + H+ + [F_actin]_L_methionine + NADPH -> [F_actin]_L_methionine_(R)_S_oxide + H2O + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 9.5 mM Tris/HCl, 45 mM KCl, 0.18 mM CaCl2, 0.18 mM MgCl, 1.1 mM ATP, 1.3 mM DTT, 1 mg/ml catalase
PH: 7.7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

[F_actin]_L_methionine + NADPH + O2 + H+ -> NADP+ + [F_actin]_L_methionine_(R)_S_oxide + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 9.5 mM Tris/HCl, 45 mM KCl, 0.18 mM CaCl2, 0.18 mM MgCl, 1.1 mM ATP, 1.3 mM DTT, 1 mg/ml catalase
PH: 7.7
Temperature: 25
uniprot:Q8TDZ2 [FAD]

O2 + [F_actin]_L_methionine + NADPH + H+ -> [F_actin]_L_methionine_(R)_S_oxide + H2O + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 9.5 mM Tris/HCl, 45 mM KCl, 0.18 mM CaCl2, 0.18 mM MgCl, 1.1 mM ATP, 1.3 mM DTT, 1 mg/ml catalase
PH: 7.7
Temperature: 25

Pathways

pathway id name
Reactome:R-HSA-109582 Hemostasis
Reactome:R-HSA-983231 Factors involved in megakaryocyte development and platelet production